[[FrontPage]] *11.1.2 Acridine orange staining Acridine orange (AO) is a vital dye that stains apoptotic cells, but not necrotic cells. Yuki Nakatani~ Tokyo Institute of Technology **126.96.36.199 Reagents -5 µg/ml acridine orange (AO) solution -medaka ringer solution **188.8.131.52. Method +Larva or adult fish are incubated in AO solution for 30 to 60 min in the dark. +Wash several times with medaka ringer solution. +Signals are detected using the green (FITC) filter set with a fluorescence microscope. **184.108.40.206 Reference Barrallo-Gimeno A, Holzschuh J, Driever W, Knapik EW. (2004). Neural crest survival and differentiation in zebrafish depends on mont blanc/tfap2a gene function. Development 131 (7), 1463-1477.