10.1.1 Preparation of mapping panel
University of Tokyo
10.1.1.1 Interbreeding of medaka strains for the generation of a mapping panel
- Heterozygous mutant carrier fish of the AA2,Qurt,drR,
Hd-rR or cab strains (derived from southern Japanese
populations) are crossed with either HNI or Kaga strains
(derived from northern populations).
- Identify the fish which are heterozygous for the mutant
allele and the wild-type allele derived from the HNI or
- Intercross heterozygous carriers and collect at least 30
mutant embryos and 30 wild-type sibling embryos.
(You must use the F1 embryos of the heterozygous
carrier fish clones.)
For mapping mutations, our protocol for BSA (bulked segregation analysis) with M-marker 2003 is described in the section 10.2.