LOCUS pBS_Tbait_olhs_G 5824 bp ds-DNA circular 06-FEB-2018 SOURCE ORGANISM COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers primer_bind 2172..2191 /locus_tag="T3" /label="T3" /ApEinfo_label="T3" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(5209..5226) /locus_tag="M13-fwd" /label="M13-fwd" /ApEinfo_label="M13-fwd" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 2134..2154 /locus_tag="M13-rev" /label="M13-rev" /ApEinfo_label="M13-rev" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(5180..5200) /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 1084..1766 /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 5384..5690 /locus_tag="F1 ori" /label="F1 ori" /ApEinfo_label="F1 ori" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5297..5365 /locus_tag="LacZ alpha" /label="LacZ alpha" /ApEinfo_label="LacZ alpha" /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_binding 2106..2128 /locus_tag="LacO" /label="LacO" /ApEinfo_label="LacO" /ApEinfo_fwdcolor="#6495ed" /ApEinfo_revcolor="#6495ed" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 327..986 /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2249..3057 /locus_tag="olhs" /label="olhs" /ApEinfo_label="olhs" /ApEinfo_fwdcolor="#ff66ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2226..2248 /locus_tag="Tbait" /label="Tbait" /ApEinfo_label="Tbait" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#008040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3077..3796 /locus_tag="EGFP" /label="EGFP" /ApEinfo_label="EGFP" /ApEinfo_fwdcolor="#80ff00" /ApEinfo_revcolor="#8000ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 4161..4976 /locus_tag="KanR" /label="KanR" /ApEinfo_label="KanR" /ApEinfo_fwdcolor="#ffff66" /ApEinfo_revcolor="#8000ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 3817..4051 /locus_tag="BGH polyA" /label="BGH polyA" /ApEinfo_label="BGH polyA" /ApEinfo_fwdcolor="#6666ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtgGCG 2221 GCCGCGGCTG CTGTCAGGGA GCTCATGGAG CTGCGTCACG TGGTCCCGAG CCAGTTTCAC 2281 AATAAGACGC CTGAATGTTG AGCCTCACTG ACGGAAACGC GCGTCCAACA GGAAACGTCT 2341 TATTTTGAAG GGTCAAACCG GAAACGGCTG TGGAGCGTTC CGCAGACTCG ACGGTTTTGT 2401 TCGTGAAGCG TCTAGAAGCA GCCAGGCTGT GAGCTTCCGC AGATATAAAG CGGCTGATCC 2461 GAGAGCAGAG ACAGTTAGAG AAAGAGAGCA GAGCGCAGAG AAGATCCAGA GGAGAAGAAA 2521 GAGAAGAAGA TCCTTCCTCT TCACAGATCA CTCCATCATC ACTCAGCTGT AAGTATTTCC 2581 TTCATTCTTT CTAGAGAAAA GGCAGGAAAG TGGAGGAAAA GCTTTGATCC AGACTATCGG 2641 GAGGAAAAGG CAGATTTGAT TAGAAACCTT TTACTTTGTT TTAATTTAAT TATTTACTTT 2701 TATTAAACTG AATTAGAAAC CTTTTTCTAT TTTAAATTTA TTTATTTATT TTTGTTAGAT 2761 TGGATAGAAA CCTTTTTGCA TGCCAATCAA ACTTTATTGC TTTAGTCGGA ACGACAGAAG 2821 CTTTGGAGAT GTTTGGTTGG TTGAAGTAGA AACCTGAAGC CTTGATGTGC TAACTTTACC 2881 GAGTAGAGAC CAGAGCAGAT GAGCGTGAGC CAGAACCGCC TGAATCCTCC TTCTAGAGGA 2941 ATCCGAGCTC TGATGCTGGA CTGGGTTCTT TCGAGACTCT GTCAGAGCGG CTGCAGAAGT 3001 TCATGTTTGT AACGTTTCCT CCCGTCTGTG TCTGCGCGTC TTTACAGCAC AAAGTCAGGA 3061 TCCaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 3121 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 3181 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 3241 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 3301 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 3361 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 3421 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 3481 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 3541 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 3601 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 3661 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 3721 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 3781 cgagctgtac aagtaaagcg gccagctagt tctagaCAGA GTCGCTGATC AGCCTCGACT 3841 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG 3901 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG 3961 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG 4021 GAAGACAATA GCAGGCATGC AAGCTGCTAG Aaagccacgt tgtgtctcaa aatctctgat 4081 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 4141 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc 4201 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 4261 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc 4321 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 4381 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 4441 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc 4501 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 4561 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 4621 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 4681 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg 4741 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 4801 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 4861 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 4921 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag 4981 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct 5041 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa 5101 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcga cctcgagggg 5161 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt 5221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 5281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 5341 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 5401 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5461 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5521 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5581 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5641 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5701 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 5761 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 5821 ttag //