NIG-Fly - Fly Stocks of National Institute of Genetics -

Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
Stock ID	GFP-IR-2
Symbol	Avic:GFPEGFP	Full Name	Green Fluorescent Protein
CG No		Old CG No
Synonyms	GFP, T:GFP
Accession No (Link to NCBI)	NM_00GFP
Inserted Chr.	ll
Insertional Mutation
Phenotype induced by Act5C-GAL4 at 28 degrees	lethal
Map Viewer
Reference [Please submit your publication]	Kohda M, Tokuzawa Y, Kishita Y, Nyuzuki H, Moriyama Y, Mizuno Y, Hirata T, Yatsuka Y, Yamashita-Sugahara Y, Nakachi Y, Kato H, Okuda A, Tamaru S, Borna NN, Banshoya K, Aigaki T, Sato-Miyata Y, Ohnuma K, Suzuki T, Nagao A, Maehata H, Matsuda F, Higasa K, Nagasaki M, Yasuda J, Yamamoto M, Fushimi T, Shimura M, Kaiho-Ichimoto K, Harashima H, Yamazaki T, Mori M, Murayama K, Ohtake A, Okazaki Y. A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. (2016) 12(1) e1005679 [ PubMed ID = 26741492 ] [ RRC reference ] Shimada N, Inami S, Sato S, Kitamoto T, Sakai T. Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain. Sci Rep (2016) 6 37255 [ PubMed ID = 27853240 ] [ RRC reference ]
Comment
Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
Primer Seq. 5'	aaggcctacatggccggaccgATGGTGAGCAAGGGCGAGGAG
Primer Seq. 3'	aatctagaggtaccCTTGAAGTTCACCTTGATGCC
Predicted Fragment Size	501
IR fragment full Seq	0001 ATGGTNANCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 0061 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCNG ATGCCACCTA 0121 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 0181 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTNTCAG CCGCTACCCC GACCACATGA 0241 AGCAGCACGA CTTCTTCAAG TCCGCCATAC CCGAAGGCTA CGTCCAGGAG CGCACCATCT 0301 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 0361 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC 0421 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA 0481 ACGGCATCAA GGTGAACTTC AAG
in silico PCR Fragment	0001 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 0061 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGC-G ATGCCACCTA 0121 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 0181 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCT-TCAG CCGCTACCCC GACCACATGA 0241 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT 0301 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC 0361 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC 0421 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA 0481 ACGGCATCAA GGTGAACTTC AAG
Assemble Data
Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
Off-target search result cv : coefficient of variation (0 mm hits / total siRNA count) mm : mismatch

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