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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
GFP-IR-1 |
| Symbol |
Avic:GFPEGFP |
Full Name |
Green Fluorescent Protein |
| CG No |
|
Old CG No |
|
| Synonyms |
GFP, T:GFP |
| Accession No (Link to NCBI) |
NM_00GFP |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Morán T, Bernués J, Azorín F.
The Drosophila histone demethylase dKDM5/LID regulates hematopoietic development.
Dev. Biol. (2015) 405(2) 260-8
[
PubMed ID = 26183107
]
[
RRC reference
]
Defaye A, Evans I, Crozatier M, Wood W, Lemaitre B, Leulier F.
Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection.
J Innate Immun (2009) 1(4) 322-34
[
PubMed ID = 20375589
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.
Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.
Cell Death Differ. (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
Leulier F, Vidal S, Saigo K, Ueda R, Lemaitre B.
Inducible expression of double-stranded RNA reveals a role for dFADD in the regulation of the antibacterial response in Drosophila adults.
Curr. Biol. (2002) 12(12) 996-1000
[
PubMed ID = 12123572
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGTGAGCAAGGGCGAGGAG |
| Primer Seq. 3' |
aatctagaggtaccCTTGAAGTTCACCTTGATGCC |
| Predicted Fragment Size |
501 |
| IR fragment full Seq |
0001 ATGGTNANCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
0061 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCNG ATGCCACCTA
0121 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
0181 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTNTCAG CCGCTACCCC GACCACATGA
0241 AGCAGCACGA CTTCTTCAAG TCCGCCATAC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
0301 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
0361 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
0421 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA
0481 ACGGCATCAA GGTGAACTTC AAG |
| in silico PCR Fragment |
0001 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
0061 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGC-G ATGCCACCTA
0121 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
0181 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCT-TCAG CCGCTACCCC GACCACATGA
0241 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
0301 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
0361 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
0421 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA
0481 ACGGCATCAA GGTGAACTTC AAG |
| Assemble Data |
GFP-IR-1.IR_full 1 ATGGTNANCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 60
||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||||
GFP-IR-1.in silico 1 ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC 60
GFP-IR-1.IR_full 61 GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCNGATGCCACCTA 120
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
GFP-IR-1.in silico 61 GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGC-GATGCCACCTA 120
GFP-IR-1.IR_full 121 CGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GFP-IR-1.in silico 121 CGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC 180
GFP-IR-1.IR_full 181 CCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTNTCAGCCGCTACCCCGACCACATGA 240
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
GFP-IR-1.in silico 181 CCTCGTGACCACCCTGACCTACGGCGTGCAGTGCT-TCAGCCGCTACCCCGACCACATGA 240
GFP-IR-1.IR_full 241 AGCAGCACGACTTCTTCAAGTCCGCCATACCCGAAGGCTACGTCCAGGAGCGCACCATCT 300
|||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
GFP-IR-1.in silico 241 AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT 300
GFP-IR-1.IR_full 301 TCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GFP-IR-1.in silico 301 TCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCC 360
GFP-IR-1.IR_full 361 TGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GFP-IR-1.in silico 361 TGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGC 420
GFP-IR-1.IR_full 421 ACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GFP-IR-1.in silico 421 ACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGA 480
GFP-IR-1.IR_full 481 ACGGCATCAAGGTGAACTTCAAG 503
|||||||||||||||||||||||
GFP-IR-1.in silico 481 ACGGCATCAAGGTGAACTTCAAG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 0 |
0 |
7 |
8 |
15 |
NM_169134.1 |
CG31556-RA (CG31556), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_164574.1 |
CG3714-RD, transcript variant D (CG3714), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_164573.1 |
CG3714-RC, transcript variant C (CG3714), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_164575.1 |
CG3714-RE, transcript variant E (CG3714), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_134974.4 |
CG3714-RB, transcript variant B (CG3714), mRNA |
| 0 |
0 |
3 |
3 |
11 |
NM_136502.2 |
CG14762-RA (CG14762), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_134495.2 |
CG12234-RA (Ranbp21), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_136900.1 |
CG8859-RA (Cyp6g2), mRNA |
| 0 |
0 |
2 |
5 |
15 |
NM_132335.2 |
CG12139-RB (CG12139), mRNA |
| 0 |
0 |
2 |
3 |
16 |
NM_001031886.1 |
CG33692-RA, transcript variant A (CG33692), mRNA |
| 0 |
0 |
2 |
3 |
16 |
NM_001031889.1 |
CG33691-RA, transcript variant A (CG33691), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_143572.2 |
CG12045-RA (CG12045), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_143764.1 |
CG18031-RA (CG18031), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_135100.2 |
CG7251-RA (CG7251), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_134519.1 |
CG11940-RA, transcript variant A (CG11940), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167957.1 |
CG12002-RB, transcript variant B (Pxn), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079549.2 |
CG7850-RA (puc), mRNA |
| 0 |
0 |
1 |
5 |
7 |
NM_139680.1 |
CG17150-RA, transcript variant A (CG17150), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_175957.1 |
CG33002-RA (mRpL27), mRNA |
| 0 |
0 |
1 |
4 |
26 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_142444.1 |
CG18599-RA (CG18599), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_169233.1 |
CG9786-RA, transcript variant A (hb), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_169234.1 |
CG9786-RB, transcript variant B (hb), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_079949.2 |
CG7586-RA (Mcr), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_167581.2 |
CG7826-RA, transcript variant A (mnb), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001032249.1 |
CG30084-RF, transcript variant F (CG30084), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_135332.2 |
CG7380-RA (CG7380), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_057495.3 |
CG5058-RB, transcript variant B (grh), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137214.3 |
CG30084-RA, transcript variant A (CG30084), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_145757.2 |
CG30084-RC, transcript variant C (CG30084), mRNA |
|
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