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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9862R-2 |
| Symbol |
Rae1 |
Full Name |
Rae1 |
| CG No |
CG9862 |
Old CG No |
CG9862 |
| Synonyms |
CG9862, dmrae1, Rae1 |
| Accession No (Link to NCBI) |
NM_137753.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Jahanshahi M, Hsiao K, Jenny A, Pfleger CM.
The Hippo Pathway Targets Rae1 to Regulate Mitosis and Organ Size and to Feed Back to Regulate Upstream Components Merlin, Hippo, and Warts.
PLoS Genet (2016) 12(8) e1006198
[
PubMed ID = 27494403
]
[
RRC reference
]
Hayashi D, Tanabe K, Katsube H, Inoue YH.
B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis in Drosophila male meiosis.
Biol Open (2016) 5(8) 1011-21
[
PubMed ID = 27402967
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTGCTGGACGTTTGCTGGTCG |
| Primer Seq. 3' |
aatctagaggtaccCTTTTGGATTCCCCGGATTCA |
| Predicted Fragment Size |
490 |
| IR fragment full Seq |
0001 GTGCTGGACG TTTGCTGGTC GGACGACGGC AGCAAAGTGT TCGTCTGCCT CCTGCGACAA
0061 GCAGGTGAAG CTCTGGGACC TCGCCTCCGA TCAAGTGATG CAAGTGGCGG CCCACGACGG
0121 TCCCGTTAAG ACGTGCCACA TGGTCAAGGG ACCCACGTAC ACCTGCCTCA TGACCGGCTC
0181 CTGGGACAAG ACCCTAAAGT TCTGGGACAC CCGTTCGCCC AATCCCATGA TGACCATCAA
0241 TCTACCCGAG CGTTGCTACT GCGCCGATGT GGAGTATACC GATGGCCGTG GTGGGCACGG
0301 CCAACAGAGG ACTCATCATA TACTCGCTGC AGAATAGCCC AACCGAGTAC AAGCGGCAGG
0361 AGAGTCCGCT GAAGTACCAG CACCGTGCCA TTTCCATTTT CCGGGACAAG AAAAAAGAGC
0421 CCACAgGCTG TGCGCTGGGC AGCATTGAGG GCCGTGTGGC CATTCAGTAT GTGAATCCGG
0481 GGAATCCAAA AG |
| in silico PCR Fragment |
0001 GTGCTGGACG TTTGCTGGTC GGACGACGGC AGCAAAGTGT TCGTC-GCCT CCTGCGACAA
0061 GCAGGTGAAG CTCTGGGACC TCGCCTCCGA TCAAGTGATG CAAGTGGCGG CCCACGACGG
0121 TCCCGTTAAG ACGTGCCACA TGGTCAAGGG ACCCACGTAC ACCTGCCTCA TGACCGGCTC
0181 CTGGGACAAG ACCCTAAAGT TCTGGGACAC CCGTTCGCCC AATCCCATGA TGACCATCAA
0241 TCTACCCGAG CGGTGCTACT GCGCCGATGT GGAGTAT-CC GATGGCCGTG GTGGGCACGG
0301 CCAACAGAGG ACTCATCATA TACTCGCTGC AGAATAGCCC AACCGAGTAC AAGCGGCAGG
0361 AGAGTCCGCT GAAGTACCAG CACCGTGCCA TTTCCATTTT CCGGGACAAG AAAAAAGAGC
0421 CCACAGGCTG TGCGCTGGGC AGCATTGAGG GCCGTGTGGC CATTCAGTAT GTGAATCCGG
0481 GGAATCCAAA AG |
| Assemble Data |
9862R-2.IR_full 1 GTGCTGGACGTTTGCTGGTCGGACGACGGCAGCAAAGTGTTCGTCTGCCTCCTGCGACAA 60
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
9862R-2.in silico 1 GTGCTGGACGTTTGCTGGTCGGACGACGGCAGCAAAGTGTTCGTC-GCCTCCTGCGACAA 60
9862R-2.IR_full 61 GCAGGTGAAGCTCTGGGACCTCGCCTCCGATCAAGTGATGCAAGTGGCGGCCCACGACGG 120
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9862R-2.in silico 61 GCAGGTGAAGCTCTGGGACCTCGCCTCCGATCAAGTGATGCAAGTGGCGGCCCACGACGG 120
9862R-2.IR_full 121 TCCCGTTAAGACGTGCCACATGGTCAAGGGACCCACGTACACCTGCCTCATGACCGGCTC 180
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9862R-2.in silico 121 TCCCGTTAAGACGTGCCACATGGTCAAGGGACCCACGTACACCTGCCTCATGACCGGCTC 180
9862R-2.IR_full 181 CTGGGACAAGACCCTAAAGTTCTGGGACACCCGTTCGCCCAATCCCATGATGACCATCAA 240
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9862R-2.in silico 181 CTGGGACAAGACCCTAAAGTTCTGGGACACCCGTTCGCCCAATCCCATGATGACCATCAA 240
9862R-2.IR_full 241 TCTACCCGAGCGTTGCTACTGCGCCGATGTGGAGTATACCGATGGCCGTGGTGGGCACGG 300
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9862R-2.in silico 241 TCTACCCGAGCGGTGCTACTGCGCCGATGTGGAGTAT-CCGATGGCCGTGGTGGGCACGG 300
9862R-2.IR_full 301 CCAACAGAGGACTCATCATATACTCGCTGCAGAATAGCCCAACCGAGTACAAGCGGCAGG 360
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9862R-2.in silico 301 CCAACAGAGGACTCATCATATACTCGCTGCAGAATAGCCCAACCGAGTACAAGCGGCAGG 360
9862R-2.IR_full 361 AGAGTCCGCTGAAGTACCAGCACCGTGCCATTTCCATTTTCCGGGACAAGAAAAAAGAGC 420
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9862R-2.in silico 361 AGAGTCCGCTGAAGTACCAGCACCGTGCCATTTCCATTTTCCGGGACAAGAAAAAAGAGC 420
9862R-2.IR_full 421 CCACAGGCTGTGCGCTGGGCAGCATTGAGGGCCGTGTGGCCATTCAGTATGTGAATCCGG 480
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9862R-2.in silico 421 CCACAGGCTGTGCGCTGGGCAGCATTGAGGGCCGTGTGGCCATTCAGTATGTGAATCCGG 480
9862R-2.IR_full 481 GGAATCCAAAAG 492
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9862R-2.in silico 481 GGAATCCAAAAG 492
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
472 |
0 |
0 |
1 |
NM_137753.2 |
CG9862-RA (Rae1), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_137721.1 |
CG18375-RA, transcript variant A (CG18375), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_169386.1 |
CG17228-RA, transcript variant A (pros), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_176459.1 |
CG17228-RD, transcript variant D (pros), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_079593.3 |
CG17228-RC, transcript variant C (pros), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_138175.2 |
CG7020-RA (DIP2), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142453.3 |
CG7125-RA, transcript variant A (PKD), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169803.3 |
CG7125-RB, transcript variant B (PKD), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169804.3 |
CG7125-RC, transcript variant C (PKD), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169805.3 |
CG7125-RD, transcript variant D (PKD), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_133075.2 |
CG6461-RA (CG6461), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_144047.1 |
CG18679-RA (CG18679), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139808.1 |
CG10064-RA (CG10064), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080041.2 |
CG7935-RA (msk), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_206365.1 |
CG11551-RA (ind), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167287.1 |
CG11715-RB, transcript variant B (Cyp4g15), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132493.2 |
CG11715-RA, transcript variant A (Cyp4g15), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_168357.2 |
CG32045-RB, transcript variant B (fry), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_001043133.1 |
CG32045-RD, transcript variant D (fry), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_142487.1 |
CG7709-RA (CG7709), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_137294.1 |
CG8317-RA (CG8317), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206775.1 |
CG12348-RG, transcript variant G (Sh), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_167592.2 |
CG12348-RD, transcript variant D (Sh), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078669.3 |
CG12348-RB, transcript variant B (Sh), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169256.1 |
CG11988-RC, transcript variant C (neur), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_169255.1 |
CG11988-RD, transcript variant D (neur), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_057304.3 |
CG11988-RA, transcript variant A (neur), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_169257.1 |
CG11988-RB, transcript variant B (neur), mRNA |
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