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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9774R-3 |
| Symbol |
rok |
Full Name |
Rho-kinase |
| CG No |
CG9774 |
Old CG No |
CG9774 |
| Synonyms |
ROCK, Drok, Rok, DROK, ROK, ROKalpha/beta, drok, Rock, RhoK, dROK, dRok, DRhk, ROCK1, CG9774, unnamed, Rhk, rok |
| Accession No (Link to NCBI) |
NM_080535.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
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Hirschhäuser A, van Cann M, Bogdan S.<br> CK1α protects WAVE from degradation to regulate cell shape and motility in the immune response.<br> J Cell Sci (2021) 134(23)
[
PubMed ID = 34730182
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Kotoula V, Moressis A, Semelidou O, Skoulakis EMC.<br> Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in <i>Drosophila</i>.<br> Proc Natl Acad Sci U S A (2017) 114(41) 10984-10989
[
PubMed ID = 28973902
]
[
RRC reference
]
Yashiro H, Loza AJ, Skeath JB, Longmore GD.<br> Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II.<br> Mol Biol Cell (2014) 25(19) 2956-69
[
PubMed ID = 25079692
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAACTGTGACCAAGCAGCGCA |
| Primer Seq. 3' |
aatctagaggtaccTATATCGCCGCCGGGCATAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAACTGTGAC CAAGCAGCGC AGCATGGATG TGGAACGAAG GCGCCGGGCG AATACGCTCG
0061 AGcGCGAGAT GCGCGATCCG ACCAGCATCT GCAATGTGGA CTGCCTGCTG GACACGGTGT
0121 CGGCCTTGGT CAGCGATTGT GACCACGAGT CCCTGAGGCG GCTTAAGAAC ATCGAGCAGT
0181 ATGCGGCCAA ATACAAACCA TTGGCAATGC AGATCAATCA GCTGCGCATG AACGTCGAGG
0241 ATTTCCATTT TATCAAACTC ATCGGCGCCG GCGCCTTTGG AGAGGTGCAG CTGGTGCGGC
0301 ACAAGTCCTC CAGCCAGGTG TACGCCATGA AGCGGCTGTC CAAATTCGAG ATGATGAAGC
0361 GACCGGACTC CGCGTTCTTC TGGGAGGAGC GACACATTAT GGCGCACGCC AACTCCGAAT
0421 GGATCGTTCA GCTGCACTTT GCATTTCAGG ATGCCAAATA CCTATACATG GTGATGGACT
0481 TTATGCCCGG CGGCGATATA |
| in silico PCR Fragment |
0001 AAACTGTGAC CAAGCAGCGC AGCATGGATG TGGAACGAAG GCGCCGGGCG AATACGCTCG
0061 AGCGCGAGAT GCGCGATCCG ACCAGCATCT GCAATGTGGA CTGCCTGCTG GACACGGTGT
0121 CGGCCTTGGT CAGCGATTGT GACCACGAGT CCCTGAGGCG GCTTAAGAAC ATCGAGCAGT
0181 ATGCGGCCAA ATACAAACCA TTGGCAATGC AGATCAATCA GCTGCGCATG AACGTCGAGG
0241 ATTTCCATTT TATCAAACTC ATCGGCGCCG GCGCCTTTGG AGAGGTGCAG CTGGTGCGGC
0301 ACAAGTCCTC CAGCCAGGTG TACGCCATGA AGCGGCTGTC CAAATTCGAG ATGATGAAGC
0361 GACCGGACTC CGCGTTCTTC TGGGAGGAGC GACACATTAT GGCGCACGCC AACTCCGAAT
0421 GGATCGTTCA GCTGCACTTT GCATTTCAGG ATGCCAAATA CCTATACATG GTGATGGACT
0481 TTATGCCCGG CGGCGATATA |
| Assemble Data |
<PRE>
9774R-3.IR_full 1 AAACTGTGACCAAGCAGCGCAGCATGGATGTGGAACGAAGGCGCCGGGCGAATACGCTCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 1 AAACTGTGACCAAGCAGCGCAGCATGGATGTGGAACGAAGGCGCCGGGCGAATACGCTCG 60
9774R-3.IR_full 61 AGCGCGAGATGCGCGATCCGACCAGCATCTGCAATGTGGACTGCCTGCTGGACACGGTGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 61 AGCGCGAGATGCGCGATCCGACCAGCATCTGCAATGTGGACTGCCTGCTGGACACGGTGT 120
9774R-3.IR_full 121 CGGCCTTGGTCAGCGATTGTGACCACGAGTCCCTGAGGCGGCTTAAGAACATCGAGCAGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 121 CGGCCTTGGTCAGCGATTGTGACCACGAGTCCCTGAGGCGGCTTAAGAACATCGAGCAGT 180
9774R-3.IR_full 181 ATGCGGCCAAATACAAACCATTGGCAATGCAGATCAATCAGCTGCGCATGAACGTCGAGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 181 ATGCGGCCAAATACAAACCATTGGCAATGCAGATCAATCAGCTGCGCATGAACGTCGAGG 240
9774R-3.IR_full 241 ATTTCCATTTTATCAAACTCATCGGCGCCGGCGCCTTTGGAGAGGTGCAGCTGGTGCGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 241 ATTTCCATTTTATCAAACTCATCGGCGCCGGCGCCTTTGGAGAGGTGCAGCTGGTGCGGC 300
9774R-3.IR_full 301 ACAAGTCCTCCAGCCAGGTGTACGCCATGAAGCGGCTGTCCAAATTCGAGATGATGAAGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 301 ACAAGTCCTCCAGCCAGGTGTACGCCATGAAGCGGCTGTCCAAATTCGAGATGATGAAGC 360
9774R-3.IR_full 361 GACCGGACTCCGCGTTCTTCTGGGAGGAGCGACACATTATGGCGCACGCCAACTCCGAAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 361 GACCGGACTCCGCGTTCTTCTGGGAGGAGCGACACATTATGGCGCACGCCAACTCCGAAT 420
9774R-3.IR_full 421 GGATCGTTCAGCTGCACTTTGCATTTCAGGATGCCAAATACCTATACATGGTGATGGACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9774R-3.in silico 421 GGATCGTTCAGCTGCACTTTGCATTTCAGGATGCCAAATACCTATACATGGTGATGGACT 480
9774R-3.IR_full 481 TTATGCCCGGCGGCGATATA 500
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9774R-3.in silico 481 TTATGCCCGGCGGCGATATA 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_080535.2 |
CG9774-RA (rok), mRNA |
| 0 |
0 |
5 |
3 |
7 |
NM_141738.3 |
CG6303-RA (Bruce), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_143177.2 |
CG5878-RA (alpha4GT2), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132089.3 |
CG3847-RA (CG3847), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_136702.2 |
CG18445-RA (CG18445), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143555.2 |
CG9717-RA (CG9717), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_140174.4 |
CG7839-RA (CG7839), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_166590.1 |
CG30412-RB, transcript variant B (CG30412), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_137933.2 |
CG30412-RA, transcript variant A (CG30412), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_001015396.1 |
CG40411-PC.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_001015395.1 |
CG40411-PE.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_001015397.1 |
CG40411-PD.3 (CG40411), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141674.2 |
CG9448-RA (CG9448), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_141325.1 |
CG2091-RA (CG2091), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_144455.2 |
CG18764-RA (CG18764), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_143729.2 |
CG17342-RA, transcript variant A (Lk6), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_169423.2 |
CG17342-RB, transcript variant B (Lk6), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139377.2 |
CG7955-RC, transcript variant C (CG7955), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167902.1 |
CG7955-RA, transcript variant A (CG7955), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167903.1 |
CG7955-RB, transcript variant B (CG7955), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137481.2 |
CG17524-RA (GstE3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206233.1 |
CG33230-RA (CG33230), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001042896.1 |
CG16863-RB, transcript variant B (CG16863), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135830.3 |
CG16863-RA, transcript variant A (CG16863), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134774.2 |
CG17646-RA, transcript variant A (CG17646), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164436.1 |
CG17646-RB, transcript variant B (CG17646), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_145193.1 |
CG30185-RA (CG30185), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135777.1 |
CG16813-RA (CG16813), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136167.1 |
CG13964-RA (CG13964), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_079446.2 |
CG8637-RA (trc), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme04350
:
TGF-beta signaling pathway
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