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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9738R-1 |
| Symbol |
Mkk4 |
Full Name |
MAP kinase kinase 4 |
| CG No |
CG9738 |
Old CG No |
CG9738 |
| Synonyms |
SEK1/MKK4, 142758_at, dMKK4, mkk4, MKK4, CT27508, CG9738, JNKK2, D-MKK4, DMKK4, Mpk4, Mkk4 |
| Accession No (Link to NCBI) |
NM_058005.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12)
[
PubMed ID = 34599799
]
[
RRC reference
]
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.<br> A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.<br> Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Sekine Y, Takagahara S, Hatanaka R, Watanabe T, Oguchi H, Noguchi T, Naguro I, Kobayashi K, Tsunoda M, Funatsu T, Nomura H, Toyoda T, Matsuki N, Kuranaga E, Miura M, Takeda K, Ichijo H.<br> p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors.<br> J Cell Sci (2011) 124(Pt 17) 3006-16
[
PubMed ID = 21878507
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTGACCCGCTGTTTTGGTGC |
| Primer Seq. 3' |
aatctagaggtaccTAGACTCTGGAATGTGGCGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTGACCCGC TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA
0061 AGTGCCACCG GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATNCGTCAC CGGGAGCGAA
0121 TTCGCCAGCA GGNCTGTGGC AAGCTACAGT TTGGTGAGGG CGGCGCTAAT ACACACACAT
0181 TTACTTCAGA CGATCTCGAG GATGAGGGCG AAATTGGACG CGGTGCATTT GGGGCAGTCA
0241 ACAAGATGAT CTTTAAGAAA TTGGACAAGG TGATGGCCGT CAAGCGCATT CGATCCACCG
0301 TAGACGAGAA GGAACAGAAG CAGCTGCTCA TGGATCTCGA GGTGGTCATG AAGTCCAACG
0361 AGTGCATTTA CATTGTTCAG TTCTATGAGG GCGCTTTTCA AGGAGGGTGA CTGCTGGATT
0421 TGTATGGAGC TGATGGACAC CTCTTTGGAC AAGTTTTACA AGTACATCTA CGAAAAGCAG
0481 CAGCGCCACA TTCCAGAGTC TA |
| in silico PCR Fragment |
0001 TGTGACCCGC TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA
0061 AGTGCCACCG GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GAT-CGTCAC CGGGAGCGAA
0121 TTCGCCAGCA GGCCTGTGGC AAGCTACAGT TTGGTGAGGG CGGCGCTAAT ACACACACAT
0181 TTACTTCAGA CGATCTCGAG GATGAGGGCG AAATTGGACG CGGTGCATTT GGGGCAGTCA
0241 ACAAGATGAC CTTTAAGAAA TTGGACAAGG TGATGGCCGT CAAGCGCATT CGATCCACCG
0301 TAGACGAGAA GGAACAGAAG CAGCTGCTCA TGGATCTCGA GGTGGTCATG AAGTCCAACG
0361 AGTGCATTTA CATTGTTCAG TTCTATG-GG GCGCTTTTCA AGGAGGGTGA CTGCTGGATT
0421 TGTATGGAGC TGATGGACAC CTCTTTGGAC AAGTTTTACA AGTACATCTA CGAAAAGCAG
0481 CAGCGCCACA TTCCAGAGTC TA |
| Assemble Data |
<PRE>
9738R-1.IR_full 1 TGTGACCCGCTGTTTTGGTGCCCAGCAGCCACAGCAAACCCCGCCGGTGGCCAGCAGTCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 1 TGTGACCCGCTGTTTTGGTGCCCAGCAGCCACAGCAAACCCCGCCGGTGGCCAGCAGTCA 60
9738R-1.IR_full 61 AGTGCCACCGGTGCCGGCAGCATCGAGCAGCAGCGCTGCCGAT<span class="snp"><tt>N</tt></span>CGTCACCGGGAGCGAA 120
|||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||
9738R-1.in silico 61 AGTGCCACCGGTGCCGGCAGCATCGAGCAGCAGCGCTGCCGAT<span class="snp"><tt>-</tt></span>CGTCACCGGGAGCGAA 120
9738R-1.IR_full 121 TTCGCCAGCAGG<span class="snp"><tt>N</tt></span>CTGTGGCAAGCTACAGTTTGGTGAGGGCGGCGCTAATACACACACAT 180
||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 121 TTCGCCAGCAGG<span class="snp"><tt>C</tt></span>CTGTGGCAAGCTACAGTTTGGTGAGGGCGGCGCTAATACACACACAT 180
9738R-1.IR_full 181 TTACTTCAGACGATCTCGAGGATGAGGGCGAAATTGGACGCGGTGCATTTGGGGCAGTCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 181 TTACTTCAGACGATCTCGAGGATGAGGGCGAAATTGGACGCGGTGCATTTGGGGCAGTCA 240
9738R-1.IR_full 241 ACAAGATGA<span class="snp"><tt>T</tt></span>CTTTAAGAAATTGGACAAGGTGATGGCCGTCAAGCGCATTCGATCCACCG 300
|||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 241 ACAAGATGA<span class="snp"><tt>C</tt></span>CTTTAAGAAATTGGACAAGGTGATGGCCGTCAAGCGCATTCGATCCACCG 300
9738R-1.IR_full 301 TAGACGAGAAGGAACAGAAGCAGCTGCTCATGGATCTCGAGGTGGTCATGAAGTCCAACG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 301 TAGACGAGAAGGAACAGAAGCAGCTGCTCATGGATCTCGAGGTGGTCATGAAGTCCAACG 360
9738R-1.IR_full 361 AGTGCATTTACATTGTTCAGTTCTATG<span class="snp"><tt>A</tt></span>GGGCGCTTTTCAAGGAGGGTGACTGCTGGATT 420
|||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
9738R-1.in silico 361 AGTGCATTTACATTGTTCAGTTCTATG<span class="snp"><tt>-</tt></span>GGGCGCTTTTCAAGGAGGGTGACTGCTGGATT 420
9738R-1.IR_full 421 TGTATGGAGCTGATGGACACCTCTTTGGACAAGTTTTACAAGTACATCTACGAAAAGCAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9738R-1.in silico 421 TGTATGGAGCTGATGGACACCTCTTTGGACAAGTTTTACAAGTACATCTACGAAAAGCAG 480
9738R-1.IR_full 481 CAGCGCCACATTCCAGAGTCTA 502
||||||||||||||||||||||
9738R-1.in silico 481 CAGCGCCACATTCCAGAGTCTA 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_058005.3 |
CG9738-RA (Mkk4), mRNA |
| 0.2 |
1 |
2 |
3 |
5 |
NM_132085.1 |
CG3823-RA (CG3823), mRNA |
| 0 |
0 |
2 |
9 |
4 |
NM_165280.1 |
CG10697-RB, transcript variant B (Ddc), mRNA |
| 0 |
0 |
2 |
9 |
4 |
NM_165279.1 |
CG10697-RC, transcript variant C (Ddc), mRNA |
| 0 |
0 |
2 |
9 |
4 |
NM_078876.4 |
CG10697-RA, transcript variant A (Ddc), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_176131.3 |
CG12052-RP, transcript variant P (lola), mRNA |
| 0 |
0 |
2 |
3 |
20 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
2 |
3 |
18 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_133088.1 |
CG6632-RA (Ing3), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_078705.2 |
CG1685-RA (pen), mRNA |
| 0 |
0 |
1 |
4 |
22 |
NM_132831.1 |
CG8184-RB (CG8184), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_139363.1 |
CG7852-RA, transcript variant A (CG7852), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_206232.1 |
CG7852-RC, transcript variant C (CG7852), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_167890.1 |
CG7852-RB, transcript variant B (CG7852), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_137564.2 |
CG7417-RA (Tab2), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169532.1 |
CG9412-RE, transcript variant E (rin), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_132246.2 |
CG10555-RA (CG10555), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169531.1 |
CG9412-RD, transcript variant D (rin), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_080168.2 |
CG9412-RB, transcript variant B (rin), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169533.1 |
CG9412-RA, transcript variant A (rin), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169530.1 |
CG9412-RC, transcript variant C (rin), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_170011.1 |
CG31285-RA (CG31285), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137340.2 |
CG8950-RA (CG8950), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057644.2 |
CG10844-RB, transcript variant B (Rya-r44F), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057645.2 |
CG10844-RC, transcript variant C (Rya-r44F), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057646.2 |
CG10844-RD, transcript variant D (Rya-r44F), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057643.2 |
CG10844-RA, transcript variant A (Rya-r44F), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_057326.4 |
CG12212-RA, transcript variant A (peb), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_001014722.1 |
CG12212-RB, transcript variant B (peb), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_206363.2 |
CG17839-RB, transcript variant B (CG17839), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04013
:
MAPK signaling pathway - fly
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