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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9623R-1 |
| Symbol |
if |
Full Name |
inflated |
| CG No |
CG9623 |
Old CG No |
CG9623 |
| Synonyms |
CG9623, alphaPS2, PS2, alpha[[PS2]], PSalpha2, CT27194, aPS2, PS2alpha, in, inf, alpha[[PS2(ms8)]], PS 2, if, alphaPS2C |
| Accession No (Link to NCBI) |
NM_078654.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Perkins AD, Ellis SJ, Asghari P, Shamsian A, Moore ED, Tanentzapf G.
Integrin-mediated adhesion maintains sarcomeric integrity.
Dev Biol (2010) 338(1) 15-27
[
PubMed ID = 19879257
]
[
RRC reference
]
Xie X, Auld VJ.
Integrins are necessary for the development and maintenance of the glial layers in the Drosophila peripheral nerve.
Development (2011) 138(17) 3813-22
[
PubMed ID = 21828098
]
[
RRC reference
]
Nonaka S, Nagaosa K, Mori T, Shiratsuchi A, Nakanishi Y.
Integrin αPS3/βν-mediated phagocytosis of apoptotic cells and bacteria in Drosophila.
J Biol Chem (2013) 288(15) 10374-80
[
PubMed ID = 23426364
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCATAACGAGCCTGATTCTG |
| Primer Seq. 3' |
aatctagaggtaccACCTCGTAGAACTCCTCGAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCATAACGA GCCTGATTCT GCTCCTGATC GCCATGTCCG CCCATGGTTA CAACATCGAC
0061 CTGCCCAGCT ACGTCCGTTT CCGGCAGTCC AGCAACTCGA TGTTCGGATT CAGCATTGCG
0121 ATGCACAAGG GTCGCAGTGG ATTCTACGGC AATCAGAACA ATGTCAGTTT GATTGTCGGA
0181 GCACCCAAAT TCGACACCTC CCGCTATCAA CAGGGCGTGA CCGAGGCAGG AGGCGTTTTC
0241 AAATGCAGCC TCAACGACGA CGACTGCAAA TTGGTGCCAT TCGACTCCAA AGGCAACAAT
0301 CGCAATGTGG ACAAGGAGGT CGTGGACAGG AAATCCTATC AATGGTTGGG CGCCACTGTG
0361 GCCACCGGTC GGGATAGCGA CTTGGTTGTG GCCTGTGCAC CGCGCTATGT CTTCCACACG
0421 ATGACGCCAT CGAGGGCgTT CCGCATTGAT CCCGTGGGCA CCTGCTTCAC CTCGCACAAC
0481 TTCGAGGAGT TCTACGAGGT |
| in silico PCR Fragment |
0001 CCCATAACGA GCCTGATTCT GCTCCTGATC GCCATGTCCG CCCATGGTTA CAACATCGAC
0061 CTGCCCAGCT ACGTCCGTTT CCGGCAGTCC AGCAACTCGA TGTTCGGATT CAGCATTGCG
0121 ATGCACAAGG GTCGCAGTGG ATTCTACGGC AATCAGAATA ATGTCAGTTT GATTGTCGGA
0181 GCACCCAAAT TCGACACCTC CCGCTATCAA CAGGGCGTGA CCGAGGCAGG AGGCGTTTTC
0241 AAATGCAGCC TCAACGACGA CGACTGCAAA TTGGTGCCAT TCGACTCCAA AGGCAACAAT
0301 CGCAATGTGG ACAAGGAGGT CGTGGACAGG AAATCCTATC AATGGTTGGG CGCCACTGTG
0361 GCAACCGGTC GGGATAGCGA CTTGGTTGTG GCCTGTGCAC CGCGCTATGT CTTCCACACG
0421 ATGACGCCAT CGAGGGCGTT CCGCATTGAT CCCGTGGGCA CCTGCTTCAC CTCGCACAAC
0481 TTCGAGGAGT TCTACGAGGT |
| Assemble Data |
9623R-1.IR_full 1 CCCATAACGAGCCTGATTCTGCTCCTGATCGCCATGTCCGCCCATGGTTACAACATCGAC 60
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9623R-1.in silico 1 CCCATAACGAGCCTGATTCTGCTCCTGATCGCCATGTCCGCCCATGGTTACAACATCGAC 60
9623R-1.IR_full 61 CTGCCCAGCTACGTCCGTTTCCGGCAGTCCAGCAACTCGATGTTCGGATTCAGCATTGCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 61 CTGCCCAGCTACGTCCGTTTCCGGCAGTCCAGCAACTCGATGTTCGGATTCAGCATTGCG 120
9623R-1.IR_full 121 ATGCACAAGGGTCGCAGTGGATTCTACGGCAATCAGAACAATGTCAGTTTGATTGTCGGA 180
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
9623R-1.in silico 121 ATGCACAAGGGTCGCAGTGGATTCTACGGCAATCAGAATAATGTCAGTTTGATTGTCGGA 180
9623R-1.IR_full 181 GCACCCAAATTCGACACCTCCCGCTATCAACAGGGCGTGACCGAGGCAGGAGGCGTTTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 181 GCACCCAAATTCGACACCTCCCGCTATCAACAGGGCGTGACCGAGGCAGGAGGCGTTTTC 240
9623R-1.IR_full 241 AAATGCAGCCTCAACGACGACGACTGCAAATTGGTGCCATTCGACTCCAAAGGCAACAAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 241 AAATGCAGCCTCAACGACGACGACTGCAAATTGGTGCCATTCGACTCCAAAGGCAACAAT 300
9623R-1.IR_full 301 CGCAATGTGGACAAGGAGGTCGTGGACAGGAAATCCTATCAATGGTTGGGCGCCACTGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 301 CGCAATGTGGACAAGGAGGTCGTGGACAGGAAATCCTATCAATGGTTGGGCGCCACTGTG 360
9623R-1.IR_full 361 GCCACCGGTCGGGATAGCGACTTGGTTGTGGCCTGTGCACCGCGCTATGTCTTCCACACG 420
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 361 GCAACCGGTCGGGATAGCGACTTGGTTGTGGCCTGTGCACCGCGCTATGTCTTCCACACG 420
9623R-1.IR_full 421 ATGACGCCATCGAGGGCGTTCCGCATTGATCCCGTGGGCACCTGCTTCACCTCGCACAAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9623R-1.in silico 421 ATGACGCCATCGAGGGCGTTCCGCATTGATCCCGTGGGCACCTGCTTCACCTCGCACAAC 480
9623R-1.IR_full 481 TTCGAGGAGTTCTACGAGGT 500
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9623R-1.in silico 481 TTCGAGGAGTTCTACGAGGT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
4 |
NM_078654.2 |
CG9623-RB, transcript variant B (if), mRNA |
| 0.41 |
2 |
3 |
4 |
4 |
NM_057349.4 |
CG31349-RB, transcript variant B (pyd), mRNA |
| 0.41 |
2 |
3 |
4 |
4 |
NM_169245.2 |
CG31349-RA, transcript variant A (pyd), mRNA |
| 0.41 |
2 |
3 |
4 |
4 |
NM_169246.2 |
CG31349-RC, transcript variant C (pyd), mRNA |
| 0 |
0 |
4 |
3 |
5 |
NM_168368.1 |
CG32044-RA (CG32044), mRNA |
| 0 |
0 |
3 |
4 |
1 |
NM_141669.2 |
CG8362-RA (nmdyn-D7), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_136094.1 |
CG10493-RA (CG10493), mRNA |
| 0 |
0 |
2 |
6 |
8 |
NM_130688.2 |
CG14423-RA (CG14423), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142928.2 |
CG10375-RA (CG10375), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_140027.1 |
CG5144-RA (CG5144), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_135753.2 |
CG9934-RA (CG9934), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165011.2 |
CG31764-RB, transcript variant B (vir-1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165010.2 |
CG31764-RA, transcript variant A (vir-1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_205966.1 |
CG31764-RC, transcript variant C (vir-1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138243.2 |
CG9128-RA (Sac1), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_166666.1 |
CG3394-RA, transcript variant A (CG3394), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_138062.2 |
CG3394-RB, transcript variant B (CG3394), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_143245.2 |
CG14247-RA (CG14247), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057531.3 |
CG6863-RA, transcript variant A (tok), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170168.1 |
CG6863-RB, transcript variant B (tok), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135232.1 |
CG17375-RA (CG17375), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_057497.3 |
CG5058-RD, transcript variant D (grh), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_057496.3 |
CG5058-RC, transcript variant C (grh), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166252.1 |
CG5058-RG, transcript variant G (grh), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_141922.1 |
CG4115-RA (CG4115), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NR_002497.1 |
CG4115-RA (CG4115), mRNA, miscRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_205987.1 |
CG32972-RA, transcript variant A (CG32972), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_176378.1 |
CG33056-RA, transcript variant A (CG33056), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206310.1 |
CG18177-RA, transcript variant A (CG18177), mRNA |
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