NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9622R-2

Symbol

CG31150

Full Name

CG31150

CG No

CG31150

Old CG No

CG9622

Synonyms

CG9625, CG9627, CG9622, CG31150

Accession No (Link to NCBI)

NM_169676.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Chen J, Honeyager SM, Schleede J, Avanesov A, Laughon A, Blair SS.<br> Crossveinless d is a vitellogenin-like lipoprotein that binds BMPs and HSPGs, and is required for normal BMP signaling in the Drosophila wing.<br> Development (2012) 139(12) 2170-6 [ PubMed ID = 22573617 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGTTGACCTATGAACCGCTG

Primer Seq. 3'

aatctagaggtaccAAGGGGATCTGATGGAAACGC

Predicted Fragment Size

IR fragment full Seq

0001 AAGTTGACCT ATGAACCGCT GGTGTTTACC AAGCGACCTC ACACTCTGAA TGGCGATGTG 
0061 GTGTTTGGCC TGGCCACCGA GGAGAGCGAG TGCCCGGAGA AGGGTTCCAC GGTGCAGTTT 
0121 GCAGCTAGAG CTGGTCCCTC GGAGCATGCC CGTGCCNTTC CTCCGCTCCG ACAAGATCTC 
0181 GCTGACGGAC ACCGACTTCT GTCCCAAGGA GGTACTGAAG TTCTCGCCCA TACCCACTTC 
0241 CAGGTATTGC ANAGCGTAGC AACTTTGAGA ACTTCACCTC GATCACACAA TACGACATGG 
0301 ACCTGAAGTT CAACAATATG CCCGCCTGGT TCGAGCTGTG GTCGAATCGA CTGGACCACT 
0361 TAGTGTCCGC CTTATCCGCC GACAAGGTGG ACAGTCTGCA TATGAGCCAG GAGATAAATA 
0421 TATCAATGCA AACTCCTCAG GACCAGTTCA GGCTGGCTGT GGAGGTCAAC GGAGTGAAGT 
0481 GGCGTTTCCA TCAGATCCCC TT

in silico PCR Fragment

0001 AAGTTGACCT ATGAACCGCT GGTGTTTACC AAGCGACCTC ACACTCTGAA TGGCGATGTG 
0061 GTGTTTGGCC TGGCCACCGA GGAGAGCGAG TGCCCGGAGA AGGGTTCCAC GGTGCAGTTT 
0121 GCAGCTAGAG CTGGTCCCTC GGAGCATGCC CGTGCC-TTC CTCCGCTCCG ACAAGATCTC 
0181 GCTGACGGAC ACCGACTTCT GTCCCAAGGA GGTACTGAAG TTCTCGCCCA TACCCACTTC 
0241 CAGGTATTGC A-AGCGTAGC AACTTTGAGA ACTTCACCTC GATCACACAA TACGACATGG 
0301 ACCTGAAGTT TGACAATATG CCCGCCTGGT TCGAGCTGTG GTCGAATCGA CTGGACCACT 
0361 TAGTGTCCGC CTTATCCGCC GACAAGGTGG ACAGTCTGCA TATGAGCCAG GAGATAAATA 
0421 TATCAATGCA AACTCCTCAG GACCAGTTCA GGCTGGCTGT GGAGGTCAAC GGAGTGAAGT 
0481 GGCGTTTCCA TCAGATCCCC TT

Assemble Data

<PRE>

9622R-2.IR_full       1   AAGTTGACCTATGAACCGCTGGTGTTTACCAAGCGACCTCACACTCTGAATGGCGATGTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     1   AAGTTGACCTATGAACCGCTGGTGTTTACCAAGCGACCTCACACTCTGAATGGCGATGTG 60


9622R-2.IR_full       61  GTGTTTGGCCTGGCCACCGAGGAGAGCGAGTGCCCGGAGAAGGGTTCCACGGTGCAGTTT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     61  GTGTTTGGCCTGGCCACCGAGGAGAGCGAGTGCCCGGAGAAGGGTTCCACGGTGCAGTTT 120


9622R-2.IR_full       121 GCAGCTAGAGCTGGTCCCTCGGAGCATGCCCGTGCC<span class="snp"><tt>N</tt></span>TTCCTCCGCTCCGACAAGATCTC 180
                          ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
9622R-2.in silico     121 GCAGCTAGAGCTGGTCCCTCGGAGCATGCCCGTGCC<span class="snp"><tt>-</tt></span>TTCCTCCGCTCCGACAAGATCTC 180


9622R-2.IR_full       181 GCTGACGGACACCGACTTCTGTCCCAAGGAGGTACTGAAGTTCTCGCCCATACCCACTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     181 GCTGACGGACACCGACTTCTGTCCCAAGGAGGTACTGAAGTTCTCGCCCATACCCACTTC 240


9622R-2.IR_full       241 CAGGTATTGCA<span class="snp"><tt>N</tt></span>AGCGTAGCAACTTTGAGAACTTCACCTCGATCACACAATACGACATGG 300
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     241 CAGGTATTGCA<span class="snp"><tt>-</tt></span>AGCGTAGCAACTTTGAGAACTTCACCTCGATCACACAATACGACATGG 300


9622R-2.IR_full       301 ACCTGAAGTT<span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span>ACAATATGCCCGCCTGGTTCGAGCTGTGGTCGAATCGACTGGACCACT 360
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     301 ACCTGAAGTT<span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span>ACAATATGCCCGCCTGGTTCGAGCTGTGGTCGAATCGACTGGACCACT 360


9622R-2.IR_full       361 TAGTGTCCGCCTTATCCGCCGACAAGGTGGACAGTCTGCATATGAGCCAGGAGATAAATA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     361 TAGTGTCCGCCTTATCCGCCGACAAGGTGGACAGTCTGCATATGAGCCAGGAGATAAATA 420


9622R-2.IR_full       421 TATCAATGCAAACTCCTCAGGACCAGTTCAGGCTGGCTGTGGAGGTCAACGGAGTGAAGT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9622R-2.in silico     421 TATCAATGCAAACTCCTCAGGACCAGTTCAGGCTGGCTGTGGAGGTCAACGGAGTGAAGT 480


9622R-2.IR_full       481 GGCGTTTCCATCAGATCCCCTT 502
                          ||||||||||||||||||||||
9622R-2.in silico     481 GGCGTTTCCATCAGATCCCCTT 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_169676.1	CG31150-RA (CG31150), mRNA
0.2	1	2	2	7	NM_138243.2	CG9128-RA (Sac1), mRNA
0	0	3	2	3	NM_080076.2	CG11770-RA (lin), mRNA
0	0	2	5	12	NM_132460.2	CG18292-RA (CG18292), mRNA
0	0	2	3	6	NM_078583.2	CG2559-RA (Lsp1alpha), mRNA
0	0	2	3	4	NM_166572.1	CG30193-RB, transcript variant B (CG30193), mRNA
0	0	2	3	4	NM_166574.1	CG30193-RE, transcript variant E (CG30193), mRNA
0	0	2	3	4	NM_166571.1	CG30193-RA, transcript variant A (CG30193), mRNA
0	0	2	3	4	NM_166575.1	CG30193-RF, transcript variant F (CG30193), mRNA
0	0	2	3	4	NM_166573.1	CG30193-RC, transcript variant C (CG30193), mRNA
0	0	2	3	4	NM_166576.1	CG30193-RD, transcript variant D (CG30193), mRNA
0	0	2	2	2	NM_170344.1	CG5588-RC, transcript variant C (Mtl), mRNA
0	0	2	2	2	NM_079809.2	CG5588-RB, transcript variant B (Mtl), mRNA
0	0	2	2	2	NM_170343.1	CG5588-RA, transcript variant A (Mtl), mRNA
0	0	1	3	6	NM_079073.1	CG8595-RA (Toll-7), mRNA
0	0	1	2	5	NM_140451.2	CG9238-RB, transcript variant B (CG9238), mRNA
0	0	1	2	5	NM_168589.1	CG9238-RA, transcript variant A (CG9238), mRNA
0	0	1	2	3	NM_140212.1	CG7543-RA (CG7543), mRNA
0	0	1	2	3	NM_169833.1	CG7717-RB, transcript variant B (Mekk1), mRNA
0	0	1	2	3	NM_142493.2	CG7717-RA, transcript variant A (Mekk1), mRNA
0	0	1	2	3	NM_134729.2	CG4749-RA (CG4749), mRNA
0	0	0	6	4	NM_139938.2	CG7213-RA (CG7213), mRNA
0	0	0	5	4	NM_057391.3	CG1977-RA (alpha-Spec), mRNA
0	0	0	4	7	NM_168670.1	CG32158-RB, transcript variant B (CG32158), mRNA
0	0	0	4	7	NM_143421.2	CG11897-RB, transcript variant B (CG11897), mRNA
0	0	0	4	7	NM_170399.1	CG11897-RA, transcript variant A (CG11897), mRNA
0	0	0	4	6	NM_164519.1	CG3558-RB, transcript variant B (CG3558), mRNA
0	0	0	4	6	NM_143807.2	CG3558-RA, transcript variant A (CG3558), mRNA
0	0	0	4	5	NM_136340.2	CG7897-RA (gp210), mRNA
0	0	0	4	2	NM_136116.4	CG17549-RA, transcript variant A (CG17549), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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