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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9553R-3 
 Symbol chic  Full Name chickadee 
 CG No CG9553  Old CG No CG9553 
 Synonyms profilin, sand, Chi, Chic, CG9553, chi, l(2)27/7, D88, chic 
 Accession No (Link to NCBI) NM_057668.4 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees early pupal lethal 
 Map Viewer
 Reference
[Please submit your publication]
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.
Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.
Dev. Biol. (2017) [ PubMed ID = 28514643 ] [ RRC reference ]

Matsukawa K, Hashimoto T, Matsumoto T, Ihara R, Chihara T, Miura M, Wakabayashi T, Iwatsubo T.
Familial ALS-linked Mutations in Profilin 1 Exacerbate TDP-43-induced Degeneration in the Retina of Drosophila Melanogaster through an Increase in the Cytoplasmic Localization of TDP-43.
J Biol Chem. (2016) [ PubMed ID = 27634045 ] [ RRC reference ]

Fairchild MJ, Smendziuk CM, Tanentzapf G.
A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis.
Development (2015) 142(2) 268-81 [ PubMed ID = 25503408 ] [ RRC reference ]

Brock AR, Wang Y, Berger S, Renkawitz-Pohl R, Han VC, Wu Y, Galko MJ.
Transcriptional regulation of Profilin during wound closure in Drosophila larvae.
J Cell Sci. (2012) 125(Pt 23) 5667-76 [ PubMed ID = 22976306 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAGCTGGCAAGATTATGTGGAC 
 Primer Seq. 3'
aatctagaggtaccTCTCCAAGTTTCTCTACCACG 
 Predicted Fragment Size
353 
 IR fragment full Seq
0001 AGCTGGCAAG ATTATGTGGA CAACCAACTC CTGGCCTCGC AGTGCGTGAC CAAGGCGTGC 
0061 ATCGCCGGCC ACGACGGCAA CATTTGGGCG CAGTCCAGTG GCTTTGAGGT GACAAAAGAG 
0121 GAGCTCTCCA AACTGATCAG CGGCTTTGAC CAGCAGGACG GTCTCACCAG CAACGGCGTG 
0181 ACACTCGCCG GCCAGCGGTA CATTTACCTT TCCGGCACAG ACCGCGTGGT GCGCGCCAAA 
0241 GCTCGGCCGG AGCGGNAGTG CACTGCATGA AGACAACACA AGCCGTGATC GTTTCCATCT 
0301 ACGAGGATCC CGTTCAGCCC CAGCAGGCCG CTTCCGTGGT AGAGAAACTT GGAGA 
 in silico PCR Fragment
0001 AGCTGGCAAG ATTATGTGGA CAACCAACTC CTGGCCTCGC AGTGCGTGAC CAAGGCGTGC 
0061 ATCGCCGGCC ACGACGGCAA CATTTGGGCG CAGTCCAGTG GCTTTGAGGT GACAAAAGAG 
0121 GAGCTCTCCA AACTGATCAG CGGCTTTGAC CAGCAGGACG GTCTCACCAG CAACGGCGTG 
0181 ACACTCGCCG GCCAGCGGTA CATTTACCTT TCCGGCACAG ACCGCGTGGT GCGCGCC-AA 
0241 GCTCGGCCGG AGCGG-AGTG CACTGCATGA AGACAACACA AGCCGTGATC GTTTCCATCT 
0301 ACGAGGATCC CGTTCAGCCC CAGCAGGCCG CTTCCGTGGT AGAGAAACTT GGAGA 
 Assemble Data

9553R-3.IR_full       1   AGCTGGCAAGATTATGTGGACAACCAACTCCTGGCCTCGCAGTGCGTGACCAAGGCGTGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9553R-3.in silico     1   AGCTGGCAAGATTATGTGGACAACCAACTCCTGGCCTCGCAGTGCGTGACCAAGGCGTGC 60


9553R-3.IR_full       61  ATCGCCGGCCACGACGGCAACATTTGGGCGCAGTCCAGTGGCTTTGAGGTGACAAAAGAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9553R-3.in silico     61  ATCGCCGGCCACGACGGCAACATTTGGGCGCAGTCCAGTGGCTTTGAGGTGACAAAAGAG 120


9553R-3.IR_full       121 GAGCTCTCCAAACTGATCAGCGGCTTTGACCAGCAGGACGGTCTCACCAGCAACGGCGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9553R-3.in silico     121 GAGCTCTCCAAACTGATCAGCGGCTTTGACCAGCAGGACGGTCTCACCAGCAACGGCGTG 180


9553R-3.IR_full       181 ACACTCGCCGGCCAGCGGTACATTTACCTTTCCGGCACAGACCGCGTGGTGCGCGCCAAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
9553R-3.in silico     181 ACACTCGCCGGCCAGCGGTACATTTACCTTTCCGGCACAGACCGCGTGGTGCGCGCC-AA 240


9553R-3.IR_full       241 GCTCGGCCGGAGCGGNAGTGCACTGCATGAAGACAACACAAGCCGTGATCGTTTCCATCT 300
                          ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
9553R-3.in silico     241 GCTCGGCCGGAGCGG-AGTGCACTGCATGAAGACAACACAAGCCGTGATCGTTTCCATCT 300


9553R-3.IR_full       301 ACGAGGATCCCGTTCAGCCCCAGCAGGCCGCTTCCGTGGTAGAGAAACTTGGAGA 355
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||
9553R-3.in silico     301 ACGAGGATCCCGTTCAGCCCCAGCAGGCCGCTTCCGTGGTAGAGAAACTTGGAGA 355

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   335  NM_057668.4  CG9553-RA, transcript variant A (chic), mRNA 
100   335  NM_205913.1  CG9553-RD, transcript variant D (chic), mRNA 
100   335  NM_164667.2  CG9553-RC, transcript variant C (chic), mRNA 
100   335  NM_134304.2  CG9553-RB, transcript variant B (chic), mRNA 
0   NM_133126.1  CG7556-RA (CG7556), mRNA 
0   NM_142691.1  CG5798-RA (CG5798), mRNA 
0   NM_164562.1  CG3964-RB, transcript variant B (CG3964), mRNA 
0   NM_134966.2  CG3964-RA, transcript variant A (CG3964), mRNA 
0   NM_136242.2  CG12050-RA (CG12050), mRNA 
0   NM_135649.1  CG4738-RA (CG4738), mRNA 
0   NM_143476.2  CG7802-RA, transcript variant A (CG7802), mRNA 
0   NM_206583.1  CG7802-RB, transcript variant B (CG7802), mRNA 
0   NM_143345.2  CG4951-RA (CG4951), mRNA 
0   11  NM_142215.2  CG5205-RA (CG5205), mRNA 
0   NM_132507.1  CG2247-RA (CG2247), mRNA 
0   NM_169844.1  CG31149-RA (CG31149), mRNA 
0   NM_206724.1  CG8497-RB, transcript variant B (Rhp), mRNA 
0   NM_078613.3  CG8497-RA, transcript variant A (Rhp), mRNA 
0   NM_136502.2  CG14762-RA (CG14762), mRNA 
0   NM_132788.2  CG9114-RA (CG9114), mRNA 
0   NM_132789.2  CG6170-RA, transcript variant A (HDAC6), mRNA 
0   NM_136608.2  CG8777-RA (CG8777), mRNA 
0   NM_170267.1  CG6154-RB, transcript variant B (CG6154), mRNA 
0   NM_143214.1  CG6154-RA, transcript variant A (CG6154), mRNA 
0   NM_169172.1  CG1070-RB, transcript variant B (Alh), mRNA 
0   NM_169171.1  CG1070-RD, transcript variant D (Alh), mRNA 
0   NM_079526.2  CG1070-RA, transcript variant A (Alh), mRNA 
0   NM_169170.1  CG1070-RC, transcript variant C (Alh), mRNA 
0   NM_165103.2  CG3506-RA (vas), mRNA 
0   NM_206294.1  CG33275-RA, transcript variant A (CG33275), mRNA 

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