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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9494R-2 |
| Symbol |
Tsp29Fa |
Full Name |
Tetraspanin 29Fa |
| CG No |
CG9494 |
Old CG No |
CG9494 |
| Synonyms |
CG9494, Dm.Tsp29Fa, Tsp29Fa |
| Accession No (Link to NCBI) |
NM_078791.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Jeibmann A, Halama K, Witte HT, Kim SN, Eikmeier K, Koos B, Klämbt C, Paulus W.
Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
J. Neurooncol. (2015) 124(3) 373-83
[
PubMed ID = 26224160
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCTCTTCCTGGCCGGAAAGTT |
| Primer Seq. 3' |
aatctagaggtaccCCGCATGGGCGGAAATGTATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCTTCTTCCT GGCCGGAAAG TTCTTCTCGA TCCCCACGTT CCTGATCGTG ATTGGATCGT
0061 TCATCATCAT AATCTCTTTC TTTGGTTGCT GGGGTGCCCT GAAGGAGAAC TATTGCCTGG
0121 TGCTCAGCTT CTCGGTCATG CTGGCCATCA TCTTCATCCT GGAGCTGGCT GCTGGCATCA
0181 GTGGCTATGT GCTGCGCAAT GACGCCTCCG ATTTGATCAA AACTTCTCTG ACTTACTCGC
0241 TGAACGAGTA TAACAGTATC AATCCAAATG CGACCACGAA ACTCTGGGAT GACATCCAGG
0301 NATGAGTTCG AGTGCTGTGG TGTGACCTCA TACAACGACT GGATCACCGC CTTcNCTAAC
0361 gGCGACCTGC CCATCTCCTG CTGCAACGTT CATGTCGGCG CAGTGGGCAC ATTCACCTGC
0421 AATAATGCTC AGTCCAGCGT GGCAGACCGG CACAAAGTTG GATGCCTCGA CGGATTCTCC
0481 GGATACATNT CNGNNCANGN GG |
| in silico PCR Fragment |
0001 GC-TCTTCCT GGCCGGAAAG TTCTTCTCGA TCCCCACGTT CCTGATCGTG ATTGGATCGT
0061 TCATCATCAT AATCTCTTTC TTTGGTTGCT GGGGTGCCCT GAAGGAGAAC TATTGCCTGG
0121 TGCTCAGCTT CTCGGTCATG CTGGCCATCA TCTTCATCCT GGAGCTGGCT GCTGGCATCA
0181 GTGGCTATGT GCTGCGCAAT GACGCCTCCG ATTTGATCAA AACTTCTCTG ACTTACTCGC
0241 TGAACGAGTA TAACAGTATC AATCCAAATG CGACCACGAA ACTCTGGGAT GACATCCAGG
0301 -ATGAGTTCG AGTGCTGTGG TGTGACCTCA TACAACGACT GGATCACCGC CTTCCCTAAC
0361 GGCGACCTGC CCATCTCCTG CTGCAACGTT CATGTCGGCG CAGTGGGCAC ATTCACCTGC
0421 AATAATGCTC AGTCCAGCGT GGCAGACCGG CACAAAGTTG GATGCCTCGA CGGATTCTCC
0481 GGATACATTT CCGCCCATGC GG |
| Assemble Data |
9494R-2.IR_full 1 GCTTCTTCCTGGCCGGAAAGTTCTTCTCGATCCCCACGTTCCTGATCGTGATTGGATCGT 60
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 1 GC-TCTTCCTGGCCGGAAAGTTCTTCTCGATCCCCACGTTCCTGATCGTGATTGGATCGT 60
9494R-2.IR_full 61 TCATCATCATAATCTCTTTCTTTGGTTGCTGGGGTGCCCTGAAGGAGAACTATTGCCTGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 61 TCATCATCATAATCTCTTTCTTTGGTTGCTGGGGTGCCCTGAAGGAGAACTATTGCCTGG 120
9494R-2.IR_full 121 TGCTCAGCTTCTCGGTCATGCTGGCCATCATCTTCATCCTGGAGCTGGCTGCTGGCATCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 121 TGCTCAGCTTCTCGGTCATGCTGGCCATCATCTTCATCCTGGAGCTGGCTGCTGGCATCA 180
9494R-2.IR_full 181 GTGGCTATGTGCTGCGCAATGACGCCTCCGATTTGATCAAAACTTCTCTGACTTACTCGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 181 GTGGCTATGTGCTGCGCAATGACGCCTCCGATTTGATCAAAACTTCTCTGACTTACTCGC 240
9494R-2.IR_full 241 TGAACGAGTATAACAGTATCAATCCAAATGCGACCACGAAACTCTGGGATGACATCCAGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 241 TGAACGAGTATAACAGTATCAATCCAAATGCGACCACGAAACTCTGGGATGACATCCAGG 300
9494R-2.IR_full 301 ATGAGTTCGAGTGCTGTGGTGTGACCTCATACAACGACTGGATCACCGCCTTCNCTAAC 359
||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
9494R-2.in silico 301 ATGAGTTCGAGTGCTGTGGTGTGACCTCATACAACGACTGGATCACCGCCTTCCCTAAC 359
9494R-2.IR_full 361 GGCGACCTGCCCATCTCCTGCTGCAACGTTCATGTCGGCGCAGTGGGCACATTCACCTGC 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 361 GGCGACCTGCCCATCTCCTGCTGCAACGTTCATGTCGGCGCAGTGGGCACATTCACCTGC 419
9494R-2.IR_full 421 AATAATGCTCAGTCCAGCGTGGCAGACCGGCACAAAGTTGGATGCCTCGACGGATTCTCC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-2.in silico 421 AATAATGCTCAGTCCAGCGTGGCAGACCGGCACAAAGTTGGATGCCTCGACGGATTCTCC 479
9494R-2.IR_full 481 GGATACATNTCNGNNCANGNGG 501
|||||||| || | || | ||
9494R-2.in silico 481 GGATACATTTCCGCCCATGCGG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078791.2 |
CG9494-RA (Tsp29Fa), mRNA |
| 0.2 |
1 |
3 |
3 |
2 |
NM_168375.2 |
CG32052-RA (CG32052), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_143116.2 |
CG31300-RA (CG31300), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_131920.2 |
CG6379-RA (CG6379), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_137703.1 |
CG15655-RA (CG15655), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_136755.2 |
CG16728-RA (CG16728), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_133094.1 |
CG12609-RA, transcript variant A (CG12609), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_167629.1 |
CG12609-RB, transcript variant B (CG12609), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_132752.2 |
CG9517-RA, transcript variant A (CG9517), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_167418.1 |
CG9517-RB, transcript variant B (CG9517), mRNA |
| 0 |
0 |
2 |
7 |
5 |
NM_165814.2 |
CG9062-RB (CG9062), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_166315.1 |
CG12758-RB, transcript variant B (sano), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_166314.1 |
CG12758-RA, transcript variant A (sano), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_166316.1 |
CG12758-RC, transcript variant C (sano), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_079064.2 |
CG12758-RD, transcript variant D (sano), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167834.1 |
CG17090-RB, transcript variant B (CG17090), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_138194.2 |
CG17090-RA, transcript variant A (CG17090), mRNA |
| 0 |
0 |
1 |
6 |
7 |
NM_078720.2 |
CG11840-RA (Spp), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_168906.1 |
CG5081-RB, transcript variant B (Syx7), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_168905.1 |
CG5081-RA, transcript variant A (Syx7), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_164459.1 |
CG31668-RA (CG31668), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_079321.2 |
CG10571-RA (ara), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_206027.1 |
CG9403-RD, transcript variant D (jing), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_170346.1 |
CG31055-RA, transcript variant A (CG31055), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_170347.1 |
CG31055-RC, transcript variant C (CG31055), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_170348.1 |
CG31055-RB, transcript variant B (CG31055), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057493.3 |
CG3178-RA (Rrp1), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_132740.1 |
CG15890-RA (CG15890), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167216.1 |
CG15312-RC, transcript variant C (CG15312), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132373.2 |
CG15312-RA, transcript variant A (CG15312), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
| N |
 |
| |
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| DB |
 |
| +- |
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| DC |
 |
| + |
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| DM |
 |
| +- |
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| VM |
 |
| +- |
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| VB |
 |
| |
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Caspase-3 signal
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