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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 9494R-1

Symbol Tsp29Fa Full Name Tetraspanin 29Fa

CG No CG9494 Old CG No CG9494

Synonyms CG9494, Dm.Tsp29Fa, Tsp29Fa

Accession No (Link to NCBI) NM_078791.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Jeibmann A, Halama K, Witte HT, Kim SN, Eikmeier K, Koos B, Klämbt C, Paulus W.
Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
J Neurooncol (2015) 124(3) 373-83 [ PubMed ID = 26224160 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCTCTTCCTGGCCGGAAAGTT

Primer Seq. 3'

aatctagaggtaccCCGCATGGGCGGAAATGTATC

Predicted Fragment Size

IR fragment full Seq

0001 GCTTCTTCCT GGCCGGAAAG TTCTTCTCGA TCCCCACGTT CCTGATCGTG ATTGGATCGT 
0061 TCATCATCAT AATCTCTTTC TTTGGTTGCT GGGGTGCCCT GAAGGAGAAC TATTGCCTGG 
0121 TGCTCAGCTT CTCGGTCATG CTGGCCATCA TCTTCATCCT GGAGCTGGCT GCTGGCATCA 
0181 GTGGCTATGT GCTGCGCAAT GACGCCTCCG ATTTGATCAA AACTTCTCTG ACTTACTCGC 
0241 TGAACGAGTA TAACAGTATC AATCCAAATG CGACCACGAA ACTCTGGGAT GACATCCAGG 
0301 NATGAGTTCG AGTGCTGTGG TGTGACCTCA TACAACGACT GGATCACCGC CTTcNCTAAC 
0361 gGCGACCTGC CCATCTCCTG CTGCAACGTT CATGTCGGCG CAGTGGGCAC ATTCACCTGC 
0421 AATAATGCTC AGTCCAGCGT GGCAGACCGG CACAAAGTTG GATGCCTCGA CGGATTCTCC 
0481 GGATACATNT CNGNNCANGN GG

in silico PCR Fragment

0001 GC-TCTTCCT GGCCGGAAAG TTCTTCTCGA TCCCCACGTT CCTGATCGTG ATTGGATCGT 
0061 TCATCATCAT AATCTCTTTC TTTGGTTGCT GGGGTGCCCT GAAGGAGAAC TATTGCCTGG 
0121 TGCTCAGCTT CTCGGTCATG CTGGCCATCA TCTTCATCCT GGAGCTGGCT GCTGGCATCA 
0181 GTGGCTATGT GCTGCGCAAT GACGCCTCCG ATTTGATCAA AACTTCTCTG ACTTACTCGC 
0241 TGAACGAGTA TAACAGTATC AATCCAAATG CGACCACGAA ACTCTGGGAT GACATCCAGG 
0301 -ATGAGTTCG AGTGCTGTGG TGTGACCTCA TACAACGACT GGATCACCGC CTTCCCTAAC 
0361 GGCGACCTGC CCATCTCCTG CTGCAACGTT CATGTCGGCG CAGTGGGCAC ATTCACCTGC 
0421 AATAATGCTC AGTCCAGCGT GGCAGACCGG CACAAAGTTG GATGCCTCGA CGGATTCTCC 
0481 GGATACATTT CCGCCCATGC GG

Assemble Data


9494R-1.IR_full       1   GCTTCTTCCTGGCCGGAAAGTTCTTCTCGATCCCCACGTTCCTGATCGTGATTGGATCGT 60
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     1   GC-TCTTCCTGGCCGGAAAGTTCTTCTCGATCCCCACGTTCCTGATCGTGATTGGATCGT 60


9494R-1.IR_full       61  TCATCATCATAATCTCTTTCTTTGGTTGCTGGGGTGCCCTGAAGGAGAACTATTGCCTGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     61  TCATCATCATAATCTCTTTCTTTGGTTGCTGGGGTGCCCTGAAGGAGAACTATTGCCTGG 120


9494R-1.IR_full       121 TGCTCAGCTTCTCGGTCATGCTGGCCATCATCTTCATCCTGGAGCTGGCTGCTGGCATCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     121 TGCTCAGCTTCTCGGTCATGCTGGCCATCATCTTCATCCTGGAGCTGGCTGCTGGCATCA 180


9494R-1.IR_full       181 GTGGCTATGTGCTGCGCAATGACGCCTCCGATTTGATCAAAACTTCTCTGACTTACTCGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     181 GTGGCTATGTGCTGCGCAATGACGCCTCCGATTTGATCAAAACTTCTCTGACTTACTCGC 240


9494R-1.IR_full       241 TGAACGAGTATAACAGTATCAATCCAAATGCGACCACGAAACTCTGGGATGACATCCAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     241 TGAACGAGTATAACAGTATCAATCCAAATGCGACCACGAAACTCTGGGATGACATCCAGG 300


9494R-1.IR_full        301 ATGAGTTCGAGTGCTGTGGTGTGACCTCATACAACGACTGGATCACCGCCTTCNCTAAC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
9494R-1.in silico      301 ATGAGTTCGAGTGCTGTGGTGTGACCTCATACAACGACTGGATCACCGCCTTCCCTAAC 359


9494R-1.IR_full       361 GGCGACCTGCCCATCTCCTGCTGCAACGTTCATGTCGGCGCAGTGGGCACATTCACCTGC 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     361 GGCGACCTGCCCATCTCCTGCTGCAACGTTCATGTCGGCGCAGTGGGCACATTCACCTGC 419


9494R-1.IR_full       421 AATAATGCTCAGTCCAGCGTGGCAGACCGGCACAAAGTTGGATGCCTCGACGGATTCTCC 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9494R-1.in silico     421 AATAATGCTCAGTCCAGCGTGGCAGACCGGCACAAAGTTGGATGCCTCGACGGATTCTCC 479


9494R-1.IR_full       481 GGATACATNTCNGNNCANGNGG 501
                          |||||||| || |  || | ||
9494R-1.in silico     481 GGATACATTTCCGCCCATGCGG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_078791.2	CG9494-RA (Tsp29Fa), mRNA
0.2	1	3	3	2	NM_168375.2	CG32052-RA (CG32052), mRNA
0	0	4	2	4	NM_143116.2	CG31300-RA (CG31300), mRNA
0	0	3	3	5	NM_131920.2	CG6379-RA (CG6379), mRNA
0	0	3	3	3	NM_137703.1	CG15655-RA (CG15655), mRNA
0	0	3	3	3	NM_136755.2	CG16728-RA (CG16728), mRNA
0	0	3	3	2	NM_133094.1	CG12609-RA, transcript variant A (CG12609), mRNA
0	0	3	3	2	NM_167629.1	CG12609-RB, transcript variant B (CG12609), mRNA
0	0	3	2	3	NM_132752.2	CG9517-RA, transcript variant A (CG9517), mRNA
0	0	3	2	3	NM_167418.1	CG9517-RB, transcript variant B (CG9517), mRNA
0	0	2	7	5	NM_165814.2	CG9062-RB (CG9062), mRNA
0	0	2	4	5	NM_166315.1	CG12758-RB, transcript variant B (sano), mRNA
0	0	2	4	5	NM_166314.1	CG12758-RA, transcript variant A (sano), mRNA
0	0	2	4	5	NM_166316.1	CG12758-RC, transcript variant C (sano), mRNA
0	0	2	4	5	NM_079064.2	CG12758-RD, transcript variant D (sano), mRNA
0	0	2	2	2	NM_167834.1	CG17090-RB, transcript variant B (CG17090), mRNA
0	0	2	2	2	NM_138194.2	CG17090-RA, transcript variant A (CG17090), mRNA
0	0	1	6	7	NM_078720.2	CG11840-RA (Spp), mRNA
0	0	1	6	6	NM_168906.1	CG5081-RB, transcript variant B (Syx7), mRNA
0	0	1	6	6	NM_168905.1	CG5081-RA, transcript variant A (Syx7), mRNA
0	0	1	6	4	NM_164459.1	CG31668-RA (CG31668), mRNA
0	0	1	5	2	NM_079321.2	CG10571-RA (ara), mRNA
0	0	1	4	6	NM_206027.1	CG9403-RD, transcript variant D (jing), mRNA
0	0	1	4	3	NM_170346.1	CG31055-RA, transcript variant A (CG31055), mRNA
0	0	1	4	3	NM_170347.1	CG31055-RC, transcript variant C (CG31055), mRNA
0	0	1	4	3	NM_170348.1	CG31055-RB, transcript variant B (CG31055), mRNA
0	0	1	3	3	NM_057493.3	CG3178-RA (Rrp1), mRNA
0	0	1	2	7	NM_132740.1	CG15890-RA (CG15890), mRNA
0	0	1	2	4	NM_167216.1	CG15312-RC, transcript variant C (CG15312), mRNA
0	0	1	2	4	NM_132373.2	CG15312-RA, transcript variant A (CG15312), mRNA

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