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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9193R-1 |
| Symbol |
mus209 |
Full Name |
mutagen-sensitive 209 |
| CG No |
CG9193 |
Old CG No |
CG9193 |
| Synonyms |
Mus209, DmPCNA, pcna, PCNA/mus209, PCNA, Pcna, l(2)02448, 53/13, l(2)s1534, l(2)56Fa, l(2)56F, CG9193, mus209, dPCNA |
| Accession No (Link to NCBI) |
NM_057557.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Zhou L, Luo H.
Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development.
J Neurosci (2013) 33(7) 2873-88
[
PubMed ID = 23407946
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTTCGAGGCACGCCTGGGT |
| Primer Seq. 3' |
aatctagaggtaccGCCCTCCTTGGTGCAGCAGAT |
| Predicted Fragment Size |
498 |
| IR fragment full Seq |
0001 ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG
0061 GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG
0121 GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT
0181 CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA
0241 TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC
0301 ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC
0361 CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG
0421 GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC
0481 TGCTGCACCA AGGAGGGC |
| in silico PCR Fragment |
0001 ATGTTCGAGG CACGCCTGGG TCAAGCCACC ATCCTGAAGA AGATCTTGGA TGCCATCAAG
0061 GATCTGCTCA ATGAGGCAAC CTTCGATTGC AGCGACTCCG GCATTCAGCT ACAGGCCATG
0121 GACAACTCCC ATGTGTCGCT TGTCTCGCTG ACCCTGCGTT CCGATGGCTT CGACAAGTTT
0181 CGCTGCGACC GCAATCTCTC CATGGGCATG AATCTGGGCA GCATGGCCAA GATTCTGAAA
0241 TGCGCCAACA ACGAGGACAA TGTGACGATG AAGGCGCAGG ATAACGCCGA CACTGTCACC
0301 ATCATGTTCG AATCGGCTAA CCAGGAGAAG GTATCGGACT ACGAGATGAA ACTGATGAAC
0361 CTCGACCAGG AGCACCTGGG CATACCGGAG ACAGACTTCT CGTGCGTGGT CCGCATGCCG
0421 GCCATGGAGT TCGCTCGCAT CTGCCGCGAT CTGGCGCAGT TCAGCGAATC CGTTGTGATC
0481 TGCTGCACCA AGGAGGGC |
| Assemble Data |
9193R-1.IR_full 1 ATGTTCGAGGCACGCCTGGGTCAAGCCACCATCCTGAAGAAGATCTTGGATGCCATCAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 1 ATGTTCGAGGCACGCCTGGGTCAAGCCACCATCCTGAAGAAGATCTTGGATGCCATCAAG 60
9193R-1.IR_full 61 GATCTGCTCAATGAGGCAACCTTCGATTGCAGCGACTCCGGCATTCAGCTACAGGCCATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 61 GATCTGCTCAATGAGGCAACCTTCGATTGCAGCGACTCCGGCATTCAGCTACAGGCCATG 120
9193R-1.IR_full 121 GACAACTCCCATGTGTCGCTTGTCTCGCTGACCCTGCGTTCCGATGGCTTCGACAAGTTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 121 GACAACTCCCATGTGTCGCTTGTCTCGCTGACCCTGCGTTCCGATGGCTTCGACAAGTTT 180
9193R-1.IR_full 181 CGCTGCGACCGCAATCTCTCCATGGGCATGAATCTGGGCAGCATGGCCAAGATTCTGAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 181 CGCTGCGACCGCAATCTCTCCATGGGCATGAATCTGGGCAGCATGGCCAAGATTCTGAAA 240
9193R-1.IR_full 241 TGCGCCAACAACGAGGACAATGTGACGATGAAGGCGCAGGATAACGCCGACACTGTCACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 241 TGCGCCAACAACGAGGACAATGTGACGATGAAGGCGCAGGATAACGCCGACACTGTCACC 300
9193R-1.IR_full 301 ATCATGTTCGAATCGGCTAACCAGGAGAAGGTATCGGACTACGAGATGAAACTGATGAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 301 ATCATGTTCGAATCGGCTAACCAGGAGAAGGTATCGGACTACGAGATGAAACTGATGAAC 360
9193R-1.IR_full 361 CTCGACCAGGAGCACCTGGGCATACCGGAGACAGACTTCTCGTGCGTGGTCCGCATGCCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 361 CTCGACCAGGAGCACCTGGGCATACCGGAGACAGACTTCTCGTGCGTGGTCCGCATGCCG 420
9193R-1.IR_full 421 GCCATGGAGTTCGCTCGCATCTGCCGCGATCTGGCGCAGTTCAGCGAATCCGTTGTGATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9193R-1.in silico 421 GCCATGGAGTTCGCTCGCATCTGCCGCGATCTGGCGCAGTTCAGCGAATCCGTTGTGATC 480
9193R-1.IR_full 481 TGCTGCACCAAGGAGGGC 498
||||||||||||||||||
9193R-1.in silico 481 TGCTGCACCAAGGAGGGC 498
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
480 |
0 |
1 |
3 |
NM_206182.1 |
CG9193-RB, transcript variant B (mus209), mRNA |
| 100 |
480 |
0 |
1 |
3 |
NM_057557.3 |
CG9193-RA, transcript variant A (mus209), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_176751.1 |
CG8557-RB, transcript variant B (CG8557), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_132990.2 |
CG8557-RA, transcript variant A (CG8557), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_169884.1 |
CG31210-RA (CG31210), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_142599.1 |
CG4836-RC, transcript variant C (CG4836), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_169883.1 |
CG4836-RA, transcript variant A (CG4836), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_169882.1 |
CG4836-RB, transcript variant B (CG4836), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
2 |
3 |
15 |
NM_134544.1 |
CG11734-RB (HERC2), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_141611.1 |
CG9746-RA (CG9746), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079771.1 |
CG11921-RA (fd96Ca), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135735.1 |
CG5525-RA (CG5525), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_137707.2 |
CG30388-RA (Magi), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_143047.2 |
CG3744-RA, transcript variant A (CG3744), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206569.1 |
CG3744-RC, transcript variant C (CG3744), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001015400.1 |
CG40410-PA.3 (CG40410), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_170177.2 |
CG3744-RB, transcript variant B (CG3744), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_138069.2 |
CG4563-RA (CG4563), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_080055.2 |
CG3895-RA (ph-d), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078963.2 |
CG7576-RA (Rab3), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176745.1 |
CG5424-RD, transcript variant D (f), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078660.2 |
CG5424-RB, transcript variant B (f), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042819.1 |
CG5424-RF, transcript variant F (f), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_078983.2 |
CG8439-RA, transcript variant A (Cct5), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_165867.1 |
CG8439-RB, transcript variant B (Cct5), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139777.1 |
CG7376-RA (CG7376), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_206301.1 |
CG7986-RC, transcript variant C (Atg18), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139927.2 |
CG7986-RA, transcript variant A (Atg18), mRNA |
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