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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9093R-3 |
| Symbol |
Tsp26A |
Full Name |
Tetraspanin 26A |
| CG No |
CG9093 |
Old CG No |
CG9093 |
| Synonyms |
CG9093, Dm.Tsp26A, Tsp26A, Tetraspanin 26A |
| Accession No (Link to NCBI) |
NM_078763.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Jeibmann A, Halama K, Witte HT, Kim SN, Eikmeier K, Koos B, Klämbt C, Paulus W.
Involvement of CD9 and PDGFR in migration is evolutionarily conserved from Drosophila glia to human glioma.
J Neurooncol (2015) 124(3) 373-83
[
PubMed ID = 26224160
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAATCAGCTGCTGTCTGAAG |
| Primer Seq. 3' |
aatctagaggtaccGCGATAGACGAGCAATTGAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAATCAGCT GCTGTCTGAA GTACCTTTTG TTCGCCAGCA ATGTGATCCT GTGGCTCNTC
0061 GGcCCTGCTG GTCCTCTCCG TTGGCATCTG GGCCTGGAGC GAGAAGGGCA TGTTCCGCAA
0121 CATTGCGCGG CTGCACTTCA TCGCCCTGGA CCCGGCCTTC GTCCTGATCA TCCTGGGAGG
0181 CGTCACCTTC CTACTGGGCT TCATGGGCAG CGTGGGCNGc CTTGCGCGAG AACACCTGCC
0241 TCCTgGNGGC GTACGCCATC TTCTTGAGTG TCTTgCTTAT CGCAGAGATC GGCTTCTGTG
0301 CCGTGGCCTT CGTGCTCAAG GACAAGGGCT GGATCAAAGA CCAGGCCACC GAGGGACTCA
0361 AGGCCTTCAT TCGCCACTAT CgNGAGGACG CAGACCAGCA GAATCTCATT GATTGGATCC
0421 AGGAGGATTG GTTGCAGTGT TGCGGCATTG ATgGTCCCAA GGACTGGGAC AGCAACAACT
0481 ACTTCAATTG CTCGTcTATN GN |
| in silico PCR Fragment |
0001 GAAATCAGCT GCTGTCTGAA GTACCTTTTG TTCGCCAGCA ATGTGATCCT GTGGCTC-TC
0061 GGCCCTGCTG GTCCTCTCCG TTGGCATCTG GGCCTGGAGC GAGAAGGGCA TGTTCCGCAA
0121 CATTGCGCGG CTGCACTTCA TCGCCCTGGA CCCGGCCTTC GTCCTGATCA TCCTGGGAGG
0181 CGTCACCTTC CTACTGGGCT TCATGGGCAG CGTGGGC-GC CTTGCGCGAG AACACCTGCC
0241 TCCTGGGGGC GTACGCCATC TTCTTGAGTG TCTTGCTTAT CGCAGAGATC GGCTTCTGTG
0301 CCGTGGCCTT CGTGCTCAAG GACAAGGGCT GGATCAAAGA CCAGGCCACC GAGGGACTCA
0361 AGGCCTTCAT TCGCCACTAT CGCGAGGACG CAGACCAGCA GAATCTCATT GATTGGATCC
0421 AGGAGGATTG GTTGCAGTGT TGCGGCATTG ATGGTCCCAA GGACTGGGAC AGCAACAACT
0481 ACTTCAATTG CTCGTCTATC GC |
| Assemble Data |
9093R-3.IR_full 1 GAAATCAGCTGCTGTCTGAAGTACCTTTTGTTCGCCAGCAATGTGATCCTGTGGCTCNTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
9093R-3.in silico 1 GAAATCAGCTGCTGTCTGAAGTACCTTTTGTTCGCCAGCAATGTGATCCTGTGGCTC-TC 60
9093R-3.IR_full 61 GGCCCTGCTGGTCCTCTCCGTTGGCATCTGGGCCTGGAGCGAGAAGGGCATGTTCCGCAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9093R-3.in silico 61 GGCCCTGCTGGTCCTCTCCGTTGGCATCTGGGCCTGGAGCGAGAAGGGCATGTTCCGCAA 120
9093R-3.IR_full 121 CATTGCGCGGCTGCACTTCATCGCCCTGGACCCGGCCTTCGTCCTGATCATCCTGGGAGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9093R-3.in silico 121 CATTGCGCGGCTGCACTTCATCGCCCTGGACCCGGCCTTCGTCCTGATCATCCTGGGAGG 180
9093R-3.IR_full 181 CGTCACCTTCCTACTGGGCTTCATGGGCAGCGTGGGCNGCCTTGCGCGAGAACACCTGCC 240
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
9093R-3.in silico 181 CGTCACCTTCCTACTGGGCTTCATGGGCAGCGTGGGC-GCCTTGCGCGAGAACACCTGCC 240
9093R-3.IR_full 241 TCCTGGNGGCGTACGCCATCTTCTTGAGTGTCTTGCTTATCGCAGAGATCGGCTTCTGTG 300
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
9093R-3.in silico 241 TCCTGGGGGCGTACGCCATCTTCTTGAGTGTCTTGCTTATCGCAGAGATCGGCTTCTGTG 300
9093R-3.IR_full 301 CCGTGGCCTTCGTGCTCAAGGACAAGGGCTGGATCAAAGACCAGGCCACCGAGGGACTCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9093R-3.in silico 301 CCGTGGCCTTCGTGCTCAAGGACAAGGGCTGGATCAAAGACCAGGCCACCGAGGGACTCA 360
9093R-3.IR_full 361 AGGCCTTCATTCGCCACTATCGNGAGGACGCAGACCAGCAGAATCTCATTGATTGGATCC 420
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
9093R-3.in silico 361 AGGCCTTCATTCGCCACTATCGCGAGGACGCAGACCAGCAGAATCTCATTGATTGGATCC 420
9093R-3.IR_full 421 AGGAGGATTGGTTGCAGTGTTGCGGCATTGATGGTCCCAAGGACTGGGACAGCAACAACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9093R-3.in silico 421 AGGAGGATTGGTTGCAGTGTTGCGGCATTGATGGTCCCAAGGACTGGGACAGCAACAACT 480
9093R-3.IR_full 481 ACTTCAATTGCTCGTCTATNGN 502
||||||||||||||||||| |
9093R-3.in silico 481 ACTTCAATTGCTCGTCTATCGC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078763.1 |
CG9093-RA (Tsp26A), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_137877.2 |
CG30265-RA (CG30265), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_137196.1 |
CG12964-RA (CG12964), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_001014464.1 |
CG10033-RJ, transcript variant J (for), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_134319.3 |
CG10033-RD, transcript variant D (for), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_058142.3 |
CG10033-RE, transcript variant E (for), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_205907.1 |
CG10033-RG, transcript variant G (for), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_058141.3 |
CG10033-RC, transcript variant C (for), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_205905.1 |
CG10033-RF, transcript variant F (for), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_141145.1 |
CG14455-RA (CG14455), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_166105.1 |
CG8205-RC, transcript variant C (fus), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079952.2 |
CG8205-RD, transcript variant D (fus), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_166108.1 |
CG8205-RF, transcript variant F (fus), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_166107.1 |
CG8205-RE, transcript variant E (fus), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167903.1 |
CG7955-RB, transcript variant B (CG7955), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167902.1 |
CG7955-RA, transcript variant A (CG7955), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_139377.2 |
CG7955-RC, transcript variant C (CG7955), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_136675.1 |
CG1625-RA (CG1625), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143298.1 |
CG3368-RA (CG3368), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136043.2 |
CG10413-RA (CG10413), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_079585.2 |
CG4591-RA (Tsp86D), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_133002.1 |
CG5800-RA (CG5800), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_134534.2 |
CG17052-RA (CG17052), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132081.2 |
CG15896-RA (CG15896), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_143236.1 |
CG14248-RA (CG14248), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141542.1 |
CG11718-RA (CG11718), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_137484.2 |
CG17530-RA (GstE6), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132385.2 |
CG2967-RA (CG2967), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132302.3 |
CG7055-RA (dalao), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135779.2 |
CG5648-RA (Prosalpha6T), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
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| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| + |
|
| VB |
 |
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
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