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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8775R-1 |
| Symbol |
CG32473 |
Full Name |
CG32473 |
| CG No |
CG32473 |
Old CG No |
CG8775 |
| Synonyms |
GM08240p, CG8775, CG11688, CG32473 |
| Accession No (Link to NCBI) |
NM_169504.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Proc. Natl. Acad. Sci. U.S.A. (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.
Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.
J. Cell Biol. (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAGTCTCTCCACAGACGACA |
| Primer Seq. 3' |
aatctagaggtaccCAGTACTTGCAGGCTGATGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAGTCTCTC CACAGACGAC AATCACCGGA TCAAGGACAC CAATCGGCCC CACAAGGCCC
0061 GCAGCAACAC GATCCTGACC CTCGCCGCAT TCTGCGTCTT TCTGCTAGTC CTCTGCGGCT
0121 TCCTGCTGGG CGCCCTCGTC TACGTCGGCA AGAATATAGC CGAGGAGCAC CAGAGGCAGC
0181 AGCACGCGAA TGCTTCCCAG TGGGCCTTGA ACTCCACCAT CCGCACAGTT TACGTGGATC
0241 GGGACCAGTT GCTATCCACC AAGCCCATTA TCAGCGTACT TTCAGATAAT CGCTTTCACA
0301 CCACTCAAGT GCCAACCAGC AAAAAAATCG AGCATAATGG CAACTGTGGC GCCCGCTGTA
0361 AAAGCCGCGA GCAAGGTGCT CCAGAATCTG GGANTCCGGC TGCCCAAACA GATTAAGCCA
0421 AGVAAGTACC GCCTTCATTT GCGCCCCGAT CTCGAAACGA AAGAACTATT CCGGCAATAA
0481 TAAGCATCAG CCTGCAAGTA CTG |
| in silico PCR Fragment |
0001 GCAGTCTCTC CACAGACGAC AATCACCGGA TCAAGGACAC CAATCGGCCC CACAAGGCCC
0061 GCAGCAACAC GATCCTGACC CTCGCCGCAT TCTGCGTCTT TCTGCTAGTC CTCTGCGGCT
0121 TCCTGCTGGG CGCCCTCGTC TACGTCGGCA AGAATATAGC CGAGGAGCAC CAGAGGCAGC
0181 AGCACGCGAA TGCTTCCCAG TGGGCCTTGA ACTCCACCAT CCGCACAGTT TACGTGGATC
0241 GGGACCAGTT GCTATCCACC AAGCCCATTA TCAGCGTACT TTCAGATAAT CGCTTTTACA
0301 CGACTCAAGT GCCAACCAGC -AAAAAATCG AGCATAATGG CAACTGTGGC GCCCGCTGTA
0361 AAAGCCGCGA GCAAGGTGCT CCAGAATCTG GGATTCCGGC TGCCCAAACA GATTAAGCCA
0421 AGCAAGTACC GCCTTCATTT GCGCCCCGAT CTCG-AACGA AAGAACTATT CCGGCAAT-A
0481 TAAGCATCAG CCTGCAAGTA CTG |
| Assemble Data |
8775R-1.IR_full 1 GCAGTCTCTCCACAGACGACAATCACCGGATCAAGGACACCAATCGGCCCCACAAGGCCC 60
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8775R-1.in silico 1 GCAGTCTCTCCACAGACGACAATCACCGGATCAAGGACACCAATCGGCCCCACAAGGCCC 60
8775R-1.IR_full 61 GCAGCAACACGATCCTGACCCTCGCCGCATTCTGCGTCTTTCTGCTAGTCCTCTGCGGCT 120
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8775R-1.in silico 61 GCAGCAACACGATCCTGACCCTCGCCGCATTCTGCGTCTTTCTGCTAGTCCTCTGCGGCT 120
8775R-1.IR_full 121 TCCTGCTGGGCGCCCTCGTCTACGTCGGCAAGAATATAGCCGAGGAGCACCAGAGGCAGC 180
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8775R-1.in silico 121 TCCTGCTGGGCGCCCTCGTCTACGTCGGCAAGAATATAGCCGAGGAGCACCAGAGGCAGC 180
8775R-1.IR_full 181 AGCACGCGAATGCTTCCCAGTGGGCCTTGAACTCCACCATCCGCACAGTTTACGTGGATC 240
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8775R-1.in silico 181 AGCACGCGAATGCTTCCCAGTGGGCCTTGAACTCCACCATCCGCACAGTTTACGTGGATC 240
8775R-1.IR_full 241 GGGACCAGTTGCTATCCACCAAGCCCATTATCAGCGTACTTTCAGATAATCGCTTTCACA 300
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8775R-1.in silico 241 GGGACCAGTTGCTATCCACCAAGCCCATTATCAGCGTACTTTCAGATAATCGCTTTTACA 300
8775R-1.IR_full 301 CCACTCAAGTGCCAACCAGCAAAAAAATCGAGCATAATGGCAACTGTGGCGCCCGCTGTA 360
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8775R-1.in silico 301 CGACTCAAGTGCCAACCAGC-AAAAAATCGAGCATAATGGCAACTGTGGCGCCCGCTGTA 360
8775R-1.IR_full 361 AAAGCCGCGAGCAAGGTGCTCCAGAATCTGGGANTCCGGCTGCCCAAACAGATTAAGCCA 420
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8775R-1.in silico 361 AAAGCCGCGAGCAAGGTGCTCCAGAATCTGGGATTCCGGCTGCCCAAACAGATTAAGCCA 420
8775R-1.IR_full 421 AGVAAGTACCGCCTTCATTTGCGCCCCGATCTCGAAACGAAAGAACTATTCCGGCAATAA 480
|| ||||||||||||||||||||||||||||||| ||||||||||||||||||||||| |
8775R-1.in silico 421 AGCAAGTACCGCCTTCATTTGCGCCCCGATCTCG-AACGAAAGAACTATTCCGGCAAT-A 480
8775R-1.IR_full 481 TAAGCATCAGCCTGCAAGTACTG 503
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8775R-1.in silico 481 TAAGCATCAGCCTGCAAGTACTG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_169504.1 |
CG32473-RC, transcript variant C (CG32473), mRNA |
| 41.28 |
199 |
1 |
1 |
1 |
NM_142019.2 |
CG32473-RB, transcript variant B (CG32473), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_166173.2 |
CG6262-RC, transcript variant C (CG6262), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_166172.3 |
CG6262-RB, transcript variant B (CG6262), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_137279.3 |
CG6262-RA, transcript variant A (CG6262), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_140430.2 |
CG6603-RA, transcript variant A (Hsc70Cb), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168573.1 |
CG6603-RB, transcript variant B (Hsc70Cb), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_168574.1 |
CG6603-RC, transcript variant C (Hsc70Cb), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165919.1 |
CG17759-RA, transcript variant A (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165923.1 |
CG17759-RG, transcript variant G (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165921.1 |
CG17759-RC, transcript variant C (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165922.1 |
CG17759-RE, transcript variant E (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_078994.2 |
CG17759-RH, transcript variant H (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165924.1 |
CG17759-RD, transcript variant D (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165920.1 |
CG17759-RB, transcript variant B (Galpha49B), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_165925.1 |
CG17759-RF, transcript variant F (Galpha49B), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_057405.2 |
CG4244-RB, transcript variant B (Su(dx)), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_143096.2 |
CG11851-RA (CG11851), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_176425.1 |
CG9821-RB, transcript variant B (CG9821), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_176424.1 |
CG9821-RA, transcript variant A (CG9821), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_057867.2 |
CG8808-RA (Pdk), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_057469.3 |
CG10718-RA (neb), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143197.1 |
CG6095-RA, transcript variant A (CG6095), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206576.1 |
CG6095-RB, transcript variant B (CG6095), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_138129.2 |
CG16896-RA (CG16896), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_206244.1 |
CG33232-RC, transcript variant C (CG33232), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_001031933.1 |
CG33232-RD, transcript variant D (CG33232), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_206246.1 |
CG33232-RA, transcript variant A (CG33232), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_206245.1 |
CG33232-RB, transcript variant B (CG33232), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_134774.2 |
CG17646-RA, transcript variant A (CG17646), mRNA |
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