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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8704R-4 |
| Symbol |
dpn |
Full Name |
deadpan |
| CG No |
CG8704 |
Old CG No |
CG8704 |
| Synonyms |
Dpn, CG8704, unnamed, 44C, anon-EST:fe1B12, dpn, DPN |
| Accession No (Link to NCBI) |
NM_057575.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.<br> DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.<br> Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Babaoğlan AB, Housden BE, Furriols M, Bray SJ.<br> Deadpan contributes to the robustness of the notch response.<br> PLoS One (2013) 8(9) e75632
[
PubMed ID = 24086596
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCGACGACGATTTCGACTGC |
| Primer Seq. 3' |
aatctagaggtaccGGAAACAAGCCACCACGGTAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCCGACGACG ATTTCGACTG CTCCAACGGC TATAGCGACA GCTATGGCTC CAATGGACGC
0061 ATGTCCAATC CCAATGGATT GTCAAAAGCA GAACTTAGAA AGACCAACAA ACCGATTATG
0121 GAGAAACGTC GCCGAGCTCG CATTAATCAC TGCCTCAACG AACTGAAGTC CCTTATCCTG
0181 GAGGCGATGA AAAAAGACCC GGCTCGTCAT ACCAAACTGG AGAAGGCCGA CATACTTGAG
0241 ATGACGGTGA AGCATCTGCA GTCGGTGCAG CGCCAGCAGC TCAACATGGC CATCCAATCG
0301 GATCCCAGCG TTGTCCAGAA GTTCAAGACG GGATTCGTTG AGTGCGCGGA GGAGGTGAAC
0361 CGCTATGTAA GCCAAATGGA TGGCATCGAT ACCGGTGTTC GTCAGCGGCT CAGCGCCCAT
0421 CTTAACCAGT GTGCCAATAG TCTGGAGCAG ATCGGTTCGA TGAGCAACTT CAGCAATGGA
0481 TACCGTGGTG GCTTGTTTCC |
| in silico PCR Fragment |
0001 TCCGACGACG ATTTCGACTG CTCCAACGGC TATAGCGACA GCTATGGCTC CAATGGACGC
0061 ATGTCCAATC CCAATGGATT GTCAAAAGCA GAACTTAGAA AGACCAACAA ACCGATTATG
0121 GAGAAACGTC GCCGAGCTCG CATTAATCAC TGCCTCAACG AACTGAAGTC CCTTATCCTG
0181 GAGGCGATGA AAAAAGACCC GGCTCGTCAT ACCAAACTGG AGAAGGCCGA CATACTTGAG
0241 ATGACGGTGA AGCATCTGCA GTCGGTGCAG CGCCAGCAGC TCAACATGGC CATCCAATCG
0301 GATCCCAGCG TTGTCCAGAA GTTCAAGACG GGATTCGTTG AGTGCGCGGA GGAGGTGAAC
0361 CGCTATGTAA GCCAAATGGA TGGCATCGAT ACCGGTGTTC GTCAGCGGCT CAGCGCCCAT
0421 CTTAACCAGT GTGCCAATAG TCTGGAGCAG ATCGGTTCGA TGAGCAACTT CAGCAATGGA
0481 TACCGTGGTG GCTTGTTTCC |
| Assemble Data |
<PRE>
8704R-4.IR_full 1 TCCGACGACGATTTCGACTGCTCCAACGGCTATAGCGACAGCTATGGCTCCAATGGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 1 TCCGACGACGATTTCGACTGCTCCAACGGCTATAGCGACAGCTATGGCTCCAATGGACGC 60
8704R-4.IR_full 61 ATGTCCAATCCCAATGGATTGTCAAAAGCAGAACTTAGAAAGACCAACAAACCGATTATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 61 ATGTCCAATCCCAATGGATTGTCAAAAGCAGAACTTAGAAAGACCAACAAACCGATTATG 120
8704R-4.IR_full 121 GAGAAACGTCGCCGAGCTCGCATTAATCACTGCCTCAACGAACTGAAGTCCCTTATCCTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 121 GAGAAACGTCGCCGAGCTCGCATTAATCACTGCCTCAACGAACTGAAGTCCCTTATCCTG 180
8704R-4.IR_full 181 GAGGCGATGAAAAAAGACCCGGCTCGTCATACCAAACTGGAGAAGGCCGACATACTTGAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 181 GAGGCGATGAAAAAAGACCCGGCTCGTCATACCAAACTGGAGAAGGCCGACATACTTGAG 240
8704R-4.IR_full 241 ATGACGGTGAAGCATCTGCAGTCGGTGCAGCGCCAGCAGCTCAACATGGCCATCCAATCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 241 ATGACGGTGAAGCATCTGCAGTCGGTGCAGCGCCAGCAGCTCAACATGGCCATCCAATCG 300
8704R-4.IR_full 301 GATCCCAGCGTTGTCCAGAAGTTCAAGACGGGATTCGTTGAGTGCGCGGAGGAGGTGAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 301 GATCCCAGCGTTGTCCAGAAGTTCAAGACGGGATTCGTTGAGTGCGCGGAGGAGGTGAAC 360
8704R-4.IR_full 361 CGCTATGTAAGCCAAATGGATGGCATCGATACCGGTGTTCGTCAGCGGCTCAGCGCCCAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 361 CGCTATGTAAGCCAAATGGATGGCATCGATACCGGTGTTCGTCAGCGGCTCAGCGCCCAT 420
8704R-4.IR_full 421 CTTAACCAGTGTGCCAATAGTCTGGAGCAGATCGGTTCGATGAGCAACTTCAGCAATGGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8704R-4.in silico 421 CTTAACCAGTGTGCCAATAGTCTGGAGCAGATCGGTTCGATGAGCAACTTCAGCAATGGA 480
8704R-4.IR_full 481 TACCGTGGTGGCTTGTTTCC 500
||||||||||||||||||||
8704R-4.in silico 481 TACCGTGGTGGCTTGTTTCC 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057575.2 |
CG8704-RA (dpn), mRNA |
| 0 |
0 |
2 |
12 |
54 |
NM_001014577.1 |
CG6494-RB, transcript variant B (h), mRNA |
| 0 |
0 |
2 |
12 |
54 |
NM_079253.3 |
CG6494-RA, transcript variant A (h), mRNA |
| 0 |
0 |
2 |
6 |
9 |
NM_079781.2 |
CG14548-RA (HLHmbeta), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_001014548.1 |
CG11406-RB, transcript variant B (CG11406), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_138053.2 |
CG11406-RA, transcript variant A (CG11406), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_141098.1 |
CG14568-RA (CG14568), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_176699.1 |
CG2977-RA, transcript variant A (inx7), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_078933.2 |
CG11194-RA (Hey), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164939.1 |
CG31871-RA (CG31871), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137124.2 |
CG10112-RA (CG10112), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079576.1 |
CG12809-RA (nerfin-2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166289.1 |
CG5186-RB, transcript variant B (slim), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136637.1 |
CG13954-RA (CG13954), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079058.2 |
CG5186-RA, transcript variant A (slim), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166591.1 |
CG30416-RA (CG30416), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_130595.2 |
CG14816-RA (CG14816), mRNA |
| 0 |
0 |
0 |
9 |
12 |
NM_078875.2 |
CG10446-RA (Side), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_080505.2 |
CG8361-RA (HLHm7), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_176526.1 |
CG32491-RV, transcript variant V (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_176523.1 |
CG32491-RY, transcript variant Y (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_163877.1 |
CG32491-RR, transcript variant R (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_163878.1 |
CG32491-RA, transcript variant A (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_080197.2 |
CG32491-RF, transcript variant F (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_163894.1 |
CG32491-RN, transcript variant N (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_163893.1 |
CG32491-RO, transcript variant O (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_163892.1 |
CG32491-RS, transcript variant S (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_176527.1 |
CG32491-RU, transcript variant U (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_176525.1 |
CG32491-RW, transcript variant W (mod(mdg4)), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_176524.1 |
CG32491-RX, transcript variant X (mod(mdg4)), mRNA |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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