|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8624R-1 |
| Symbol |
melt |
Full Name |
melted |
| CG No |
CG8624 |
Old CG No |
CG8624 |
| Synonyms |
1441/14, anon-WO03014152.10, CG8624, melt, melted, dMelt |
| Accession No (Link to NCBI) |
NM_079229.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.<br> Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.<br> Dev Biol (2017) 427(1) 61-71
[
PubMed ID = 28514643
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAACAAAGTGCTCGCCAAACGC |
| Primer Seq. 3' |
aatctagaggtaccATCACGGACTCCGTATGAGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AACAAAGTGC TCGCCAAACG CGATCTCTCA AGAGCCGGAG ATCTCTTTTC CGTGCCGGAT
0061 GCAGATATAG TCGACGACAT CACGGAGGTG CTTTCCGAGA TCAGTCCGAT CATCTCGCAC
0121 GCTGACTATG TGAAGAACAA CAATGACCAG AGTGTGGTGG AGATCTGTGT GACACGGGTG
0181 CTATCCTGCA TCCGGGAAAC GAAAACTGCG GAGCGATACT GTGCTGCTCT GGTGGATCTT
0241 CTGAGAACCT GTCTGCTTTG GAACCTCCAA CCCTCGGGAA CAACAAAGGA AGAGCCACCC
0301 CATGCCAAGA TTGCTGCCGA TATCATCTCT AGCATTTTTC TGAACTATGA CAAAAAAGAA
0361 CTGATGAAAA TAGCCCTTNC CCGTCGCCGT GCAGTTCCTT CCGAAGGGAA ACCGTGAGCT
0421 ATCGCGAAAC TTGGCCAGTT ACCTCTCACT GGCGGCCATC GATCACGCCG GCTTGCTGAG
0481 CCCTCATACG GAGTCCGTGA T |
| in silico PCR Fragment |
0001 AACAAAGTGC TCGCCAAACG CGATCTCTCA AGAGCCGGAG ATCTCTTTTC CGTGCCGGAT
0061 GCAGATATAG TCGACGACAT CACGGAGGTG CTTTCCGAGA TCAGTCCGAT CATCTCGCAC
0121 GCGGACTATG TGAAGAACAA CAATGACCAG AGTGTGGTGG AGATCTGTGT GACACGGGTG
0181 CTATCCTGCA TCCGGGAAAC GAAAACTGCG GAGCGATACT GTGCTGCTCT GGTGGATCTT
0241 CTGAGGACCT GTCTGCTATG GAACCTCCAA CCCTCGGGAA CAACAAAGGA AGAGCCACCC
0301 CATGCCAAGA TTGCTGCCGA TATCATCTCT AGCATTTTCC TGAACTATGA CAAAAAAGAA
0361 CTGATGAAAA TAGCCCTT-C CCGTCGCCGT GCAGTTCCTT CCGAAGGGAA ACCGTGAGCT
0421 ATCGCGAAAC TTGGCCAGTT ACCTCTCACT GGCGGCCATC GATCACGCCG GCTTGCTGAG
0481 CCCTCATACG GAGTCCGTGA T |
| Assemble Data |
<PRE>
8624R-1.IR_full 1 AACAAAGTGCTCGCCAAACGCGATCTCTCAAGAGCCGGAGATCTCTTTTCCGTGCCGGAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 1 AACAAAGTGCTCGCCAAACGCGATCTCTCAAGAGCCGGAGATCTCTTTTCCGTGCCGGAT 60
8624R-1.IR_full 61 GCAGATATAGTCGACGACATCACGGAGGTGCTTTCCGAGATCAGTCCGATCATCTCGCAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 61 GCAGATATAGTCGACGACATCACGGAGGTGCTTTCCGAGATCAGTCCGATCATCTCGCAC 120
8624R-1.IR_full 121 GC<span class="snp"><tt>T</tt></span>GACTATGTGAAGAACAACAATGACCAGAGTGTGGTGGAGATCTGTGTGACACGGGTG 180
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 121 GC<span class="snp"><tt>G</tt></span>GACTATGTGAAGAACAACAATGACCAGAGTGTGGTGGAGATCTGTGTGACACGGGTG 180
8624R-1.IR_full 181 CTATCCTGCATCCGGGAAACGAAAACTGCGGAGCGATACTGTGCTGCTCTGGTGGATCTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 181 CTATCCTGCATCCGGGAAACGAAAACTGCGGAGCGATACTGTGCTGCTCTGGTGGATCTT 240
8624R-1.IR_full 241 CTGAG<span class="snp"><tt>A</tt></span>ACCTGTCTGCT<span class="snp"><tt>T</tt></span>TGGAACCTCCAACCCTCGGGAACAACAAAGGAAGAGCCACCC 300
|||||<span class="snp"><tt> </tt></span>|||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 241 CTGAG<span class="snp"><tt>G</tt></span>ACCTGTCTGCT<span class="snp"><tt>A</tt></span>TGGAACCTCCAACCCTCGGGAACAACAAAGGAAGAGCCACCC 300
8624R-1.IR_full 301 CATGCCAAGATTGCTGCCGATATCATCTCTAGCATTTT<span class="snp"><tt>T</tt></span>CTGAACTATGACAAAAAAGAA 360
||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||
8624R-1.in silico 301 CATGCCAAGATTGCTGCCGATATCATCTCTAGCATTTT<span class="snp"><tt>C</tt></span>CTGAACTATGACAAAAAAGAA 360
8624R-1.IR_full 361 CTGATGAAAATAGCCCTT<span class="snp"><tt>N</tt></span>CCCGTCGCCGTGCAGTTCCTTCCGAAGGGAAACCGTGAGCT 420
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 361 CTGATGAAAATAGCCCTT<span class="snp"><tt>-</tt></span>CCCGTCGCCGTGCAGTTCCTTCCGAAGGGAAACCGTGAGCT 420
8624R-1.IR_full 421 ATCGCGAAACTTGGCCAGTTACCTCTCACTGGCGGCCATCGATCACGCCGGCTTGCTGAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8624R-1.in silico 421 ATCGCGAAACTTGGCCAGTTACCTCTCACTGGCGGCCATCGATCACGCCGGCTTGCTGAG 480
8624R-1.IR_full 481 CCCTCATACGGAGTCCGTGAT 501
|||||||||||||||||||||
8624R-1.in silico 481 CCCTCATACGGAGTCCGTGAT 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_001014572.1 |
CG8624-RB, transcript variant B (melt), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_079229.1 |
CG8624-RA, transcript variant A (melt), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_164466.1 |
CG32463-RA (CG32463), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001038737.1 |
CG34052-RA (CG34052), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_134774.2 |
CG17646-RA, transcript variant A (CG17646), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_164436.1 |
CG17646-RB, transcript variant B (CG17646), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132859.2 |
CG9066-RA (CG9066), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142458.2 |
CG7670-RA (CG7670), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136382.1 |
CG30445-RA (Tdc1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_078867.2 |
CG5526-RA (Dhc36C), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057429.3 |
CG9703-RA, transcript variant A (Axs), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038760.1 |
CG9703-RB, transcript variant B (Axs), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_079065.3 |
CG15113-RA (5-HT1B), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_164671.1 |
CG9088-RB, transcript variant B (lid), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_078762.4 |
CG9088-RA, transcript variant A (lid), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_164860.1 |
CG4389-RB, transcript variant B (CG4389), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_135455.2 |
CG4389-RA, transcript variant A (CG4389), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170540.1 |
CG31004-RB, transcript variant B (CG31004), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_170539.1 |
CG31004-RA, transcript variant A (CG31004), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166991.2 |
CG32779-RA (CG32779), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_136673.2 |
CG1698-RA (CG1698), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_143224.2 |
CG5455-RA, transcript variant A (CG5455), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_170273.1 |
CG5455-RB, transcript variant B (CG5455), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_170274.1 |
CG5455-RC, transcript variant C (CG5455), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166988.3 |
CG2947-RA, transcript variant A (CG2947), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_132403.3 |
CG17255-RA, transcript variant A (CG17255), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_167231.3 |
CG17255-RB, transcript variant B (CG17255), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001014719.1 |
CG2947-RC, transcript variant C (CG2947), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166989.3 |
CG2947-RB, transcript variant B (CG2947), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_130718.3 |
CG32789-RA (CG32789), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
