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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8544R-2 |
| Symbol |
sd |
Full Name |
scalloped |
| CG No |
CG8544 |
Old CG No |
CG8544 |
| Synonyms |
sd, CG8544, Sd, EP(X)1088, DROEXO, DROEXO2, sp, l(1)G0239, l(1)G0262, l(1)G0309, l(1)G0483, clone 2.14, anon-EST:Liang-2.14, l(1)G0315, l(1)III, l(1)HF394, EP1088, spatula, scalloped, SD |
| Accession No (Link to NCBI) |
NM_078614.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.<br> Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.<br> Dev Biol (2017) 427(1) 61-71
[
PubMed ID = 28514643
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.<br> DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.<br> Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.<br> A transcriptional network controlling glial development in the Drosophila visual system.<br> Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Doggett K, Grusche FA, Richardson HE, Brumby AM.<br> Loss of the Drosophila cell polarity regulator Scribbled promotes epithelial tissue overgrowth and cooperation with oncogenic Ras-Raf through impaired Hippo pathway signaling.<br> BMC Dev Biol (2011) 11 57
[
PubMed ID = 21955824
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGAACAACCTGAGCTGCAGCG |
| Primer Seq. 3' |
aatctagaggtaccGAAATCTTGGGACGTGCTTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGAACAACCT GAGCTGCAGC NNGTTGGAAG TTGCCGAGAA GACAGAACAA CAGGCAGTTG
0061 GACCCGGCAC CATACCATCA CCGTGGACAC CAGTGAATGC CGGTCCTCCA GGCGCACTTG
0121 GATCGGCAGA CACAAATGGC AGCATGGTGG ATAGCAAAAA CCTGGATGTC GGTGATATGA
0181 GCGATGACGA AAAGGACTTG TCATCCGCTG ATGCCGAAGG TGTATGGAGT CCAGATATCG
0241 AGCAGAGCTT TCAAGAGGCT TTATCTATAT ATCCGCCGTG NNCGGACGTA GAAAAATCAT
0301 TTTATCCGAC GAGGGTAAAA TGTACGGTCG CAACGAGCTA ATCGCACGAT ATATAAAACT
0361 GCGCACAGGC AAAACGAGAA CCAGGAAGCA AGTCAGTTCG CACATCCAAG TGCTGGCTCG
0421 CCGTAAACTC CGCGAGATCC AGGCGAAAAT CAAAGTGCAA TTCTGGCAAC CTGGACTACA
0481 GCCAAGCACG TCCCAAGATT TC |
| in silico PCR Fragment |
0001 AGAACAACCT GAGCTGCAGC GAGTTGGAAG TTGCCGAGAA GACAGAACAA CAGGCAGTTG
0061 GACCCGGCAC CATACCATCA CCGTGGACAC CAGTGAATGC CGGTCCTCCA GGCGCACTTG
0121 GATCGGCAGA CACAAATGGC AGCATGGTGG ATAGCAAAAA CCTGGATGTC GGTGATATGA
0181 GCGATGACGA AAAGGACTTG TCATCCGCTG ATGCCGAAGG TGTATGGAGT CCAGATATCG
0241 AGCAGAGCTT TCAAGAGGCT TTATCTATAT ATCCGCCGTG --CGGACGTA GAAAAATCAT
0301 TTTATCCGAC GAGGGTAAAA TGTACGGTCG CAACGAGCTA ATCGCACGAT ATATAAAACT
0361 GCGCACAGGC AAAACGAGAA CCAGGAAGCA AGTCAGTTCG CACATCCAAG TGCTGGCTCG
0421 CCGTAAACTC CGCGAGATCC AGGCGAAAAT CAAAGTGCAA TTCTGGCAAC CTGGACTACA
0481 GCCAAGCACG TCCCAAGATT TC |
| Assemble Data |
<PRE>
8544R-2.IR_full 1 AGAACAACCTGAGCTGCAGC<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GTTGGAAGTTGCCGAGAAGACAGAACAACAGGCAGTTG 60
||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 1 AGAACAACCTGAGCTGCAGC<span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>GTTGGAAGTTGCCGAGAAGACAGAACAACAGGCAGTTG 60
8544R-2.IR_full 61 GACCCGGCACCATACCATCACCGTGGACACCAGTGAATGCCGGTCCTCCAGGCGCACTTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 61 GACCCGGCACCATACCATCACCGTGGACACCAGTGAATGCCGGTCCTCCAGGCGCACTTG 120
8544R-2.IR_full 121 GATCGGCAGACACAAATGGCAGCATGGTGGATAGCAAAAACCTGGATGTCGGTGATATGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 121 GATCGGCAGACACAAATGGCAGCATGGTGGATAGCAAAAACCTGGATGTCGGTGATATGA 180
8544R-2.IR_full 181 GCGATGACGAAAAGGACTTGTCATCCGCTGATGCCGAAGGTGTATGGAGTCCAGATATCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 181 GCGATGACGAAAAGGACTTGTCATCCGCTGATGCCGAAGGTGTATGGAGTCCAGATATCG 240
8544R-2.IR_full 241 AGCAGAGCTTTCAAGAGGCTTTATCTATATATCCGCCGTG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CGGACGTAGAAAAATCAT 300
||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||||||||
8544R-2.in silico 241 AGCAGAGCTTTCAAGAGGCTTTATCTATATATCCGCCGTG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CGGACGTAGAAAAATCAT 300
8544R-2.IR_full 301 TTTATCCGACGAGGGTAAAATGTACGGTCGCAACGAGCTAATCGCACGATATATAAAACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 301 TTTATCCGACGAGGGTAAAATGTACGGTCGCAACGAGCTAATCGCACGATATATAAAACT 360
8544R-2.IR_full 361 GCGCACAGGCAAAACGAGAACCAGGAAGCAAGTCAGTTCGCACATCCAAGTGCTGGCTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 361 GCGCACAGGCAAAACGAGAACCAGGAAGCAAGTCAGTTCGCACATCCAAGTGCTGGCTCG 420
8544R-2.IR_full 421 CCGTAAACTCCGCGAGATCCAGGCGAAAATCAAAGTGCAATTCTGGCAACCTGGACTACA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8544R-2.in silico 421 CCGTAAACTCCGCGAGATCCAGGCGAAAATCAAAGTGCAATTCTGGCAACCTGGACTACA 480
8544R-2.IR_full 481 GCCAAGCACGTCCCAAGATTTC 502
||||||||||||||||||||||
8544R-2.in silico 481 GCCAAGCACGTCCCAAGATTTC 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_078614.3 |
CG8544-RB, transcript variant B (sd), mRNA |
| 87.55 |
422 |
3 |
4 |
3 |
NM_206727.1 |
CG8544-RC, transcript variant C (sd), mRNA |
| 87.34 |
421 |
2 |
4 |
3 |
NM_167465.1 |
CG8544-RA, transcript variant A (sd), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_140046.2 |
CG4452-RA, transcript variant A (CG4452), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_168328.1 |
CG4452-RB, transcript variant B (CG4452), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_168329.1 |
CG4452-RC, transcript variant C (CG4452), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_166935.1 |
CG2924-RA, transcript variant A (CG2924), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_130638.2 |
CG2924-RC, transcript variant C (CG2924), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_137076.1 |
CG6701-RA (CG6701), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140953.2 |
CG5605-RA, transcript variant A (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168848.1 |
CG5605-RF, transcript variant F (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_176369.1 |
CG5605-RG, transcript variant G (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168847.1 |
CG5605-RE, transcript variant E (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168845.1 |
CG5605-RB, transcript variant B (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_168846.1 |
CG5605-RC, transcript variant C (eRF1), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165733.1 |
CG2264-RE, transcript variant E (CG2264), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165731.1 |
CG2264-RC, transcript variant C (CG2264), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165732.1 |
CG2264-RD, transcript variant D (CG2264), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_165730.1 |
CG2264-RA, transcript variant A (CG2264), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_136704.2 |
CG2264-RB, transcript variant B (CG2264), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_168703.2 |
CG11661-RC, transcript variant C (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_176340.1 |
CG11661-RF, transcript variant F (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_176341.1 |
CG11661-RG, transcript variant G (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_168702.1 |
CG11661-RB, transcript variant B (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_168701.2 |
CG11661-RA, transcript variant A (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_168704.2 |
CG11661-RE, transcript variant E (Nc73EF), mRNA |
| 0 |
0 |
1 |
9 |
3 |
NM_176342.1 |
CG11661-RH, transcript variant H (Nc73EF), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_079091.2 |
CG12781-RA, transcript variant A (nahoda), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_166569.1 |
CG12781-RB, transcript variant B (nahoda), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136258.1 |
CG8674-RA (l(2)k14505), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04391
:
Hippo signaling pathway - fly
dme04392
:
Hippo signaling pathway - multiple species
|
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