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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8491R-1 
 Symbol kto  Full Name kohtalo 
 CG No CG8491  Old CG No CG8491 
 Synonyms MED12, Med12, Kto, CG8491, Srb8/KTO/TRAP230, Trap230, dTRAP230, SRB8, Kto/Med12, kto 
 Accession No (Link to NCBI) NM_080047.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Mao F, Yang X, Fu L, Lv X, Zhang Z, Wu W, Yang S, Zhou Z, Zhang L, Zhao Y.
The Kto-Skd complex can regulate ptc expression by interacting with Cubitus interruptus (Ci) in the Hedgehog signaling pathway.
J. Biol. Chem. (2014) 289(32) 22333-41 [ PubMed ID = 24962581 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, D├╝rrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTGGGTCCGCCGGACATCTATC 
 Primer Seq. 3'
aatctagaggtaccCTCGGCAGCCGGGTCGTAAAT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TGGGTCCGCC GGACATCTAT CCGCAGGACG CGAAGCAGCG AGAGGACGAG CTGACGCCCA 
0061 CGAATGTGAA GCACGGATTC ACGACGACGC CACCGCTGTC CGATGAATTC GGTACGGCCC 
0121 ACAACTCGAA TGTGAACGCC AGCAAAGTGA GCGCATTCTT TAGCGGCGTC CTGGCCAAGA 
0181 AGGAGGAGCT GATGACCCTG CCGGACACGG GGCGCAAGAA GCAGCAGATC AACTGCAAGG 
0241 ACAACTTCTG GCCCGTTTCG CCGCGTCGCA AGTGTACGGT GGACGCCTGG TTTAAGGACC 
0301 TCGCCGGTAA CAAACCCCTA CTTAGCCTAG CCAAACGGGC TCCATCGTTC AACAAGAAGG 
0361 AGGAGATCTT CATTACTCTG TGCGAGAACC AGGTGAACAT GCAACGCGCC ACCTGGTTCA 
0421 TCAAGCTGAG CGCCNGCCTA TACGCTTAGC TTCACCGAGT CTAAGAACAA AAAGCGATCC 
0481 ATTTACGACC CGGCTGCCGA G 
 in silico PCR Fragment
0001 TGGGTCCGCC GGACATCTAT CCGCAGGACG CGAAGCAGCG AGAGGACGAG CTGACGCCCA 
0061 CGAATGTGAA GCACGGATTC ACGACGACGC CACCGCTGTC CGATGAATTC GGTACGGCCC 
0121 ACAACTCGAA TGTGAACGCC AGCAAAGTGA GCGCATTCTT TAGCGGCGTC CTGGCCAAGA 
0181 AGGAGGAGCT GATGACCCTG CCGGACACGG GGCGCAAGAA GCAGCAGATC AACTGCAAGG 
0241 ACAACTTCTG GCCCGTTTCG CCGCGTCGCA AGTGTACGGT GGACGCCTGG TTTAAGGACC 
0301 TCGCCGGTAA CAAACCCCTA CTTAGCCTAG CCAAACGGGC TCCATCGTTC AACAAGAAGG 
0361 AGGAGATCTT CATTACTCTG TGCGAGAACC AGGTGAACAT GCAACGCGCC ACCTGGTTCA 
0421 TCAAGCTGAG CGCC-GCCTA TACGCTTAGC TTCACCGAGT CTAAGAACAA AAAGCGATCC 
0481 ATTTACGACC CGGCTGCCGA G 
 Assemble Data

8491R-1.IR_full       1   TGGGTCCGCCGGACATCTATCCGCAGGACGCGAAGCAGCGAGAGGACGAGCTGACGCCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     1   TGGGTCCGCCGGACATCTATCCGCAGGACGCGAAGCAGCGAGAGGACGAGCTGACGCCCA 60


8491R-1.IR_full       61  CGAATGTGAAGCACGGATTCACGACGACGCCACCGCTGTCCGATGAATTCGGTACGGCCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     61  CGAATGTGAAGCACGGATTCACGACGACGCCACCGCTGTCCGATGAATTCGGTACGGCCC 120


8491R-1.IR_full       121 ACAACTCGAATGTGAACGCCAGCAAAGTGAGCGCATTCTTTAGCGGCGTCCTGGCCAAGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     121 ACAACTCGAATGTGAACGCCAGCAAAGTGAGCGCATTCTTTAGCGGCGTCCTGGCCAAGA 180


8491R-1.IR_full       181 AGGAGGAGCTGATGACCCTGCCGGACACGGGGCGCAAGAAGCAGCAGATCAACTGCAAGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     181 AGGAGGAGCTGATGACCCTGCCGGACACGGGGCGCAAGAAGCAGCAGATCAACTGCAAGG 240


8491R-1.IR_full       241 ACAACTTCTGGCCCGTTTCGCCGCGTCGCAAGTGTACGGTGGACGCCTGGTTTAAGGACC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     241 ACAACTTCTGGCCCGTTTCGCCGCGTCGCAAGTGTACGGTGGACGCCTGGTTTAAGGACC 300


8491R-1.IR_full       301 TCGCCGGTAACAAACCCCTACTTAGCCTAGCCAAACGGGCTCCATCGTTCAACAAGAAGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     301 TCGCCGGTAACAAACCCCTACTTAGCCTAGCCAAACGGGCTCCATCGTTCAACAAGAAGG 360


8491R-1.IR_full       361 AGGAGATCTTCATTACTCTGTGCGAGAACCAGGTGAACATGCAACGCGCCACCTGGTTCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     361 AGGAGATCTTCATTACTCTGTGCGAGAACCAGGTGAACATGCAACGCGCCACCTGGTTCA 420


8491R-1.IR_full       421 TCAAGCTGAGCGCCNGCCTATACGCTTAGCTTCACCGAGTCTAAGAACAAAAAGCGATCC 480
                          |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
8491R-1.in silico     421 TCAAGCTGAGCGCC-GCCTATACGCTTAGCTTCACCGAGTCTAAGAACAAAAAGCGATCC 480


8491R-1.IR_full       481 ATTTACGACCCGGCTGCCGAG 501
                          |||||||||||||||||||||
8491R-1.in silico     481 ATTTACGACCCGGCTGCCGAG 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_080047.2  CG8491-RA (kto), mRNA 
0.2   NM_166339.1  CG7225-RA (wbl), mRNA 
0   NM_168391.1  CG11989-RC, transcript variant C (Ard1), mRNA 
0   NM_140121.1  CG11989-RA, transcript variant A (Ard1), mRNA 
0   NM_168390.1  CG11989-RB, transcript variant B (Ard1), mRNA 
0   NM_168393.1  CG11989-RE, transcript variant E (Ard1), mRNA 
0   NM_168392.1  CG11989-RD, transcript variant D (Ard1), mRNA 
0   NM_135273.4  CG5229-RA (chm), mRNA 
0   NM_132615.1  CG4395-RA (CG4395), mRNA 
0   NM_165522.1  CG11112-RB, transcript variant B (CG11112), mRNA 
0   NM_001014603.1  CG32490-RJ, transcript variant J (cpx), mRNA 
0   NM_001014602.1  CG32490-RK, transcript variant K (cpx), mRNA 
0   NM_080280.2  CG32490-RE, transcript variant E (cpx), mRNA 
0   NM_164327.2  CG32490-RA, transcript variant A (cpx), mRNA 
0   NM_164330.2  CG32490-RC, transcript variant C (cpx), mRNA 
0   NM_164331.2  CG32490-RH, transcript variant H (cpx), mRNA 
0   NM_164328.2  CG32490-RG, transcript variant G (cpx), mRNA 
0   NM_164329.2  CG32490-RI, transcript variant I (cpx), mRNA 
0   NM_142201.1  CG14868-RA (CG14868), mRNA 
0   NM_131926.1  CG15570-RA (CG15570), mRNA 
0   NM_137901.1  CG9896-RA (CG9896), mRNA 
0   NM_080299.2  CG18531-RA (Gr2a), mRNA 
0   NM_168060.1  CG14991-RA, transcript variant A (Fit1), mRNA 
0   NM_139601.2  CG14991-RB, transcript variant B (Fit1), mRNA 
0   NM_165146.1  CG4894-RC, transcript variant C (Ca-alpha1D), mRNA 
0   NM_134429.2  CG4894-RA, transcript variant A (Ca-alpha1D), mRNA 
0   NM_080365.2  CG4894-RB, transcript variant B (Ca-alpha1D), mRNA 
0   NM_165147.1  CG4894-RD, transcript variant D (Ca-alpha1D), mRNA 
0   NM_169212.1  CG7549-RB, transcript variant B (CG7549), mRNA 
0   NM_141337.1  CG2031-RA (Hpr1), mRNA 

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