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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8486R-3 |
| Symbol |
CG8486 |
Full Name |
CG8486 |
| CG No |
CG8486 |
Old CG No |
CG8486 |
| Synonyms |
clone 1.32, anon-EST:Liang-1.32, CG8486 |
| Accession No (Link to NCBI) |
NM_135344.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Lee J, Cabrera AJH, Nguyen CMT, Kwon YV.
Dissemination of RasV12-transformed cells requires the mechanosensitive channel Piezo.
Nat Commun (2020) 11(1) 3568
[
PubMed ID = 32678085
]
[
RRC reference
]
Tokusumi Y, Tokusumi T, Schulz RA.
The nociception genes painless and Piezo are required for the cellular immune response of Drosophila larvae to wasp parasitization.
Biochem Biophys Res Commun (2017) 486(4) 893-897
[
PubMed ID = 28342875
]
[
RRC reference
]
Kim SE, Coste B, Chadha A, Cook B, Patapoutian A.
The role of Drosophila Piezo in mechanical nociception.
Nature (2012) 483(7388) 209-12
[
PubMed ID = 22343891
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGATGTTCTTTGTGTCGCCC |
| Primer Seq. 3' |
aatctagaggtaccTGGCTGATCATGGACACTCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGATGTTCT TTGTGTCGCC CTTTGTTCCG CTGGCCACGC GACGCAACTT CAAAGGATCT
0061 GTGACTGCCT TCTTCATCAT CCTGCTGACG TTGAGCACGC TGGTCCTCTT GGGTCACATA
0121 ACGCTACAGA TTCTGGCGGT CAGCCTCACG CTGCCCATCT ACAATTGCTC GTTCAGTGAG
0181 CGTCTGCTGC GTCACATTGG CTTCGTGAGC TTTATTGATC TACAGCCATT TGCCATCATC
0241 GAATGGCTGG TGCCTGAGGT GTTGGTTTTC GCCACCTCCC TGGGTTCGTA TCTCACGGTG
0301 AAGCGAGTGG CCTCACAGCC CGTCGGCGCC CGAGCAGCTG GAGAACGGCG AAGTGGTCGA
0361 TGGTCAGGCG GAAAATGCCC AGACATCCTC TCAGCCATCT GCCGCAGATG CCAATGGAGG
0421 AGATGTGCAA CAGGCCACGG TCACCTACGC CGCTGCAGCA ACAGCAGCAG CAGCTNGAGG
0481 AAACGAGTGT CCATGATCAG CCA |
| in silico PCR Fragment |
0001 TTGATGTTCT TTGTGTCGCC CTTTGTTCCG CTGGCCACGC GACGCAACTT CAAAGGATCT
0061 GTGACTGCCT TCTTCATCAT CCTGCTGACG TTGAGCACGC TGGTCCTCTT GGGTCACATA
0121 ACGCTACAGA TTCTGGCGGT CAGCCTCACG CTGCCCATCT ACAATTGCTC GTTCAGTGAG
0181 CGTCTGCTGC GTCACATTGG CTTCGTGAGC TTTATTGATC TACAGCCATT TGCCATCATC
0241 GAATGGCTAG TGCCTGAGGT GTTGGTTTTC GCCACCTCCC TGGGTTCGTA TCTCACGGTG
0301 AAGCGAGTGG CCTCACAGCC CGTCGGCG-C CGAGCAGCTG GAGAACGGCG AGGTGGTCGA
0361 TGGCCAGGCG GAAAACGCGC AGACATCTTC TCAGCCATCT GCCGCAGATG CCAATGGAGG
0421 AGATGTGCAA CAGGCCACGG TCACC-ACGC CGCTGCAGCA ACAGCAGCAG CAGCT-GAGG
0481 AAACGAGTGT CCATGATCAG CCA |
| Assemble Data |
8486R-3.IR_full 1 TTGATGTTCTTTGTGTCGCCCTTTGTTCCGCTGGCCACGCGACGCAACTTCAAAGGATCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-3.in silico 1 TTGATGTTCTTTGTGTCGCCCTTTGTTCCGCTGGCCACGCGACGCAACTTCAAAGGATCT 60
8486R-3.IR_full 61 GTGACTGCCTTCTTCATCATCCTGCTGACGTTGAGCACGCTGGTCCTCTTGGGTCACATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-3.in silico 61 GTGACTGCCTTCTTCATCATCCTGCTGACGTTGAGCACGCTGGTCCTCTTGGGTCACATA 120
8486R-3.IR_full 121 ACGCTACAGATTCTGGCGGTCAGCCTCACGCTGCCCATCTACAATTGCTCGTTCAGTGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-3.in silico 121 ACGCTACAGATTCTGGCGGTCAGCCTCACGCTGCCCATCTACAATTGCTCGTTCAGTGAG 180
8486R-3.IR_full 181 CGTCTGCTGCGTCACATTGGCTTCGTGAGCTTTATTGATCTACAGCCATTTGCCATCATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-3.in silico 181 CGTCTGCTGCGTCACATTGGCTTCGTGAGCTTTATTGATCTACAGCCATTTGCCATCATC 240
8486R-3.IR_full 241 GAATGGCTGGTGCCTGAGGTGTTGGTTTTCGCCACCTCCCTGGGTTCGTATCTCACGGTG 300
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-3.in silico 241 GAATGGCTAGTGCCTGAGGTGTTGGTTTTCGCCACCTCCCTGGGTTCGTATCTCACGGTG 300
8486R-3.IR_full 301 AAGCGAGTGGCCTCACAGCCCGTCGGCGCCCGAGCAGCTGGAGAACGGCGAAGTGGTCGA 360
|||||||||||||||||||||||||||| |||||||||||||||||||||| ||||||||
8486R-3.in silico 301 AAGCGAGTGGCCTCACAGCCCGTCGGCG-CCGAGCAGCTGGAGAACGGCGAGGTGGTCGA 360
8486R-3.IR_full 361 TGGTCAGGCGGAAAATGCCCAGACATCCTCTCAGCCATCTGCCGCAGATGCCAATGGAGG 420
||| ||||||||||| || |||||||| ||||||||||||||||||||||||||||||||
8486R-3.in silico 361 TGGCCAGGCGGAAAACGCGCAGACATCTTCTCAGCCATCTGCCGCAGATGCCAATGGAGG 420
8486R-3.IR_full 421 AGATGTGCAACAGGCCACGGTCACCTACGCCGCTGCAGCAACAGCAGCAGCAGCTNGAGG 480
||||||||||||||||||||||||| ||||||||||||||||||||||||||||| ||||
8486R-3.in silico 421 AGATGTGCAACAGGCCACGGTCACC-ACGCCGCTGCAGCAACAGCAGCAGCAGCT-GAGG 480
8486R-3.IR_full 481 AAACGAGTGTCCATGATCAGCCA 503
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8486R-3.in silico 481 AAACGAGTGTCCATGATCAGCCA 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
24 |
NM_135344.2 |
CG8486-RA, transcript variant A (CG8486), mRNA |
| 100 |
482 |
0 |
2 |
24 |
NM_164795.2 |
CG8486-RB, transcript variant B (CG8486), mRNA |
| 100 |
482 |
0 |
2 |
19 |
NM_001042881.1 |
CG8486-RC, transcript variant C (CG8486), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 1.65 |
8 |
54 |
180 |
419 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 1.65 |
8 |
54 |
180 |
419 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 1.65 |
8 |
30 |
64 |
120 |
NM_166446.1 |
CG9696-RD, transcript variant D (dom), mRNA |
| 1.65 |
8 |
30 |
64 |
120 |
NM_080094.2 |
CG9696-RA, transcript variant A (dom), mRNA |
| 1.65 |
8 |
3 |
11 |
23 |
NM_164599.1 |
CG2950-RC, transcript variant C (CG2950), mRNA |
| 1.45 |
7 |
26 |
81 |
177 |
NM_143575.2 |
CG12071-RA, transcript variant A (CG12071), mRNA |
| 1.45 |
7 |
26 |
81 |
177 |
NM_176592.1 |
CG12071-RB, transcript variant B (CG12071), mRNA |
| 1.24 |
6 |
39 |
114 |
255 |
NM_131975.3 |
CG32767-RA, transcript variant A (CG32767), mRNA |
| 1.24 |
6 |
39 |
114 |
255 |
NM_206628.1 |
CG32767-RB, transcript variant B (CG32767), mRNA |
| 1.24 |
6 |
30 |
111 |
316 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 1.24 |
6 |
21 |
38 |
79 |
NM_079938.3 |
CG2102-RA (cas), mRNA |
| 1.24 |
6 |
20 |
76 |
182 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 1.24 |
6 |
20 |
76 |
182 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 1.24 |
6 |
20 |
69 |
223 |
NM_206286.1 |
CG10107-RC, transcript variant C (CG10107), mRNA |
| 1.24 |
6 |
20 |
69 |
223 |
NM_139799.3 |
CG10107-RA, transcript variant A (CG10107), mRNA |
| 1.24 |
6 |
14 |
60 |
143 |
NM_167026.2 |
CG6824-RB, transcript variant B (ovo), mRNA |
| 1.24 |
6 |
14 |
60 |
141 |
NM_167027.2 |
CG6824-RC, transcript variant C (ovo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 1.03 |
5 |
28 |
81 |
215 |
NM_080364.3 |
CG5461-RA, transcript variant A (bun), mRNA |
| 1.03 |
5 |
28 |
75 |
197 |
NM_001042894.1 |
CG5461-RF, transcript variant F (bun), mRNA |
| 1.03 |
5 |
26 |
75 |
213 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 1.03 |
5 |
24 |
83 |
168 |
NM_167000.1 |
CG32778-RA (CG32778), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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