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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8486R-1 |
| Symbol |
CG8486 |
Full Name |
CG8486 |
| CG No |
CG8486 |
Old CG No |
CG8486 |
| Synonyms |
clone 1.32, anon-EST:Liang-1.32, CG8486 |
| Accession No (Link to NCBI) |
NM_135344.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
McKelvey EGZ, Gyles JP, Michie K, Barquín Pancorbo V, Sober L, Kruszewski LE, Chan A, Fabre CCG.<br> Drosophila females receive male substrate-borne signals through specific leg neurons during courtship.<br> Curr Biol (2021) 31(17) 3894-3904.e5
[
PubMed ID = 34174209
]
[
RRC reference
]
Chen X, Wanggou S, Bodalia A, Zhu M, Dong W, Fan JJ, Yin WC, Min HK, Hu M, Draghici D, Dou W, Li F, Coutinho FJ, Whetstone H, Kushida MM, Dirks PB, Song Y, Hui CC, Sun Y, Wang LY, Li X, Huang X.<br> A Feedforward Mechanism Mediated by Mechanosensitive Ion Channel PIEZO1 and Tissue Mechanics Promotes Glioma Aggression.<br> Neuron (2018) 100(4) 799-815.e7
[
PubMed ID = 30344046
]
[
RRC reference
]
Tokusumi Y, Tokusumi T, Schulz RA.<br> The nociception genes painless and Piezo are required for the cellular immune response of Drosophila larvae to wasp parasitization.<br> Biochem Biophys Res Commun (2017) 486(4) 893-897
[
PubMed ID = 28342875
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGATGTTCTTTGTGTCGCCC |
| Primer Seq. 3' |
aatctagaggtaccTGGCTGATCATGGACACTCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGATGTTCT TTGTGTCGCC CTTTGTTCCG CTGGCCACGC GACGCAACTT CAAAGGATCT
0061 GTGACTGCCT TCTTCATCAT CCTGCTGACG TTGAGCACGC TGGTCCTCTT GGGTCACATA
0121 ACGCTACAGA TTCTGGCGGT CAGCCTCACG CTGCCCATCT ACAATTGCTC GTTCAGTGAG
0181 CGTCTGCTGC GTCACATTGG CTTCGTGAGC TTTATTGATC TACAGCCATT TGCCATCATC
0241 GAATGGCTGG TGCCTGAGGT GTTGGTTTTC GCCACCTCCC TGGGTTCGTA TCTCACGGTG
0301 AAGCGAGTGG CCTCACAGCC CGTCGGCGCC CGAGCAGCTG GAGAACGGCG AAGTGGTCGA
0361 TGGTCAGGCG GAAAATGCCC AGACATCCTC TCAGCCATCT GCCGCAGATG CCAATGGAGG
0421 AGATGTGCAA CAGGCCACGG TCACCTACGC CGCTGCAGCA ACAGCAGCAG CAGCTNGAGG
0481 AAACGAGTGT CCATGATCAG CCA |
| in silico PCR Fragment |
0001 TTGATGTTCT TTGTGTCGCC CTTTGTTCCG CTGGCCACGC GACGCAACTT CAAAGGATCT
0061 GTGACTGCCT TCTTCATCAT CCTGCTGACG TTGAGCACGC TGGTCCTCTT GGGTCACATA
0121 ACGCTACAGA TTCTGGCGGT CAGCCTCACG CTGCCCATCT ACAATTGCTC GTTCAGTGAG
0181 CGTCTGCTGC GTCACATTGG CTTCGTGAGC TTTATTGATC TACAGCCATT TGCCATCATC
0241 GAATGGCTAG TGCCTGAGGT GTTGGTTTTC GCCACCTCCC TGGGTTCGTA TCTCACGGTG
0301 AAGCGAGTGG CCTCACAGCC CGTCGGCG-C CGAGCAGCTG GAGAACGGCG AGGTGGTCGA
0361 TGGCCAGGCG GAAAACGCGC AGACATCTTC TCAGCCATCT GCCGCAGATG CCAATGGAGG
0421 AGATGTGCAA CAGGCCACGG TCACC-ACGC CGCTGCAGCA ACAGCAGCAG CAGCT-GAGG
0481 AAACGAGTGT CCATGATCAG CCA |
| Assemble Data |
<PRE>
8486R-1.IR_full 1 TTGATGTTCTTTGTGTCGCCCTTTGTTCCGCTGGCCACGCGACGCAACTTCAAAGGATCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-1.in silico 1 TTGATGTTCTTTGTGTCGCCCTTTGTTCCGCTGGCCACGCGACGCAACTTCAAAGGATCT 60
8486R-1.IR_full 61 GTGACTGCCTTCTTCATCATCCTGCTGACGTTGAGCACGCTGGTCCTCTTGGGTCACATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-1.in silico 61 GTGACTGCCTTCTTCATCATCCTGCTGACGTTGAGCACGCTGGTCCTCTTGGGTCACATA 120
8486R-1.IR_full 121 ACGCTACAGATTCTGGCGGTCAGCCTCACGCTGCCCATCTACAATTGCTCGTTCAGTGAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-1.in silico 121 ACGCTACAGATTCTGGCGGTCAGCCTCACGCTGCCCATCTACAATTGCTCGTTCAGTGAG 180
8486R-1.IR_full 181 CGTCTGCTGCGTCACATTGGCTTCGTGAGCTTTATTGATCTACAGCCATTTGCCATCATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-1.in silico 181 CGTCTGCTGCGTCACATTGGCTTCGTGAGCTTTATTGATCTACAGCCATTTGCCATCATC 240
8486R-1.IR_full 241 GAATGGCT<span class="snp"><tt>G</tt></span>GTGCCTGAGGTGTTGGTTTTCGCCACCTCCCTGGGTTCGTATCTCACGGTG 300
||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
8486R-1.in silico 241 GAATGGCT<span class="snp"><tt>A</tt></span>GTGCCTGAGGTGTTGGTTTTCGCCACCTCCCTGGGTTCGTATCTCACGGTG 300
8486R-1.IR_full 301 AAGCGAGTGGCCTCACAGCCCGTCGGCG<span class="snp"><tt>C</tt></span>CCGAGCAGCTGGAGAACGGCGA<span class="snp"><tt>A</tt></span>GTGGTCGA 360
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||
8486R-1.in silico 301 AAGCGAGTGGCCTCACAGCCCGTCGGCG<span class="snp"><tt>-</tt></span>CCGAGCAGCTGGAGAACGGCGA<span class="snp"><tt>G</tt></span>GTGGTCGA 360
8486R-1.IR_full 361 TGG<span class="snp"><tt>T</tt></span>CAGGCGGAAAA<span class="snp"><tt>T</tt></span>GC<span class="snp"><tt>C</tt></span>CAGACATC<span class="snp"><tt>C</tt></span>TCTCAGCCATCTGCCGCAGATGCCAATGGAGG 420
|||<span class="snp"><tt> </tt></span>|||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
8486R-1.in silico 361 TGG<span class="snp"><tt>C</tt></span>CAGGCGGAAAA<span class="snp"><tt>C</tt></span>GC<span class="snp"><tt>G</tt></span>CAGACATC<span class="snp"><tt>T</tt></span>TCTCAGCCATCTGCCGCAGATGCCAATGGAGG 420
8486R-1.IR_full 421 AGATGTGCAACAGGCCACGGTCACC<span class="snp"><tt>T</tt></span>ACGCCGCTGCAGCAACAGCAGCAGCAGCT<span class="snp"><tt>N</tt></span>GAGG 480
|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||
8486R-1.in silico 421 AGATGTGCAACAGGCCACGGTCACC<span class="snp"><tt>-</tt></span>ACGCCGCTGCAGCAACAGCAGCAGCAGCT<span class="snp"><tt>-</tt></span>GAGG 480
8486R-1.IR_full 481 AAACGAGTGTCCATGATCAGCCA 503
|||||||||||||||||||||||
8486R-1.in silico 481 AAACGAGTGTCCATGATCAGCCA 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
24 |
NM_135344.2 |
CG8486-RA, transcript variant A (CG8486), mRNA |
| 100 |
482 |
0 |
2 |
24 |
NM_164795.2 |
CG8486-RB, transcript variant B (CG8486), mRNA |
| 100 |
482 |
0 |
2 |
19 |
NM_001042881.1 |
CG8486-RC, transcript variant C (CG8486), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 2.69 |
13 |
134 |
436 |
823 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 1.65 |
8 |
54 |
180 |
419 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 1.65 |
8 |
54 |
180 |
419 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 1.65 |
8 |
30 |
64 |
120 |
NM_166446.1 |
CG9696-RD, transcript variant D (dom), mRNA |
| 1.65 |
8 |
30 |
64 |
120 |
NM_080094.2 |
CG9696-RA, transcript variant A (dom), mRNA |
| 1.65 |
8 |
3 |
11 |
23 |
NM_164599.1 |
CG2950-RC, transcript variant C (CG2950), mRNA |
| 1.45 |
7 |
26 |
81 |
177 |
NM_143575.2 |
CG12071-RA, transcript variant A (CG12071), mRNA |
| 1.45 |
7 |
26 |
81 |
177 |
NM_176592.1 |
CG12071-RB, transcript variant B (CG12071), mRNA |
| 1.24 |
6 |
39 |
114 |
255 |
NM_131975.3 |
CG32767-RA, transcript variant A (CG32767), mRNA |
| 1.24 |
6 |
39 |
114 |
255 |
NM_206628.1 |
CG32767-RB, transcript variant B (CG32767), mRNA |
| 1.24 |
6 |
30 |
111 |
316 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 1.24 |
6 |
21 |
38 |
79 |
NM_079938.3 |
CG2102-RA (cas), mRNA |
| 1.24 |
6 |
20 |
76 |
182 |
NM_001038742.1 |
CG6824-RD, transcript variant D (ovo), mRNA |
| 1.24 |
6 |
20 |
76 |
182 |
NM_080338.3 |
CG6824-RA, transcript variant A (ovo), mRNA |
| 1.24 |
6 |
20 |
69 |
223 |
NM_206286.1 |
CG10107-RC, transcript variant C (CG10107), mRNA |
| 1.24 |
6 |
20 |
69 |
223 |
NM_139799.3 |
CG10107-RA, transcript variant A (CG10107), mRNA |
| 1.24 |
6 |
14 |
60 |
143 |
NM_167026.2 |
CG6824-RB, transcript variant B (ovo), mRNA |
| 1.24 |
6 |
14 |
60 |
141 |
NM_167027.2 |
CG6824-RC, transcript variant C (ovo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 1.03 |
5 |
33 |
99 |
264 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 1.03 |
5 |
28 |
81 |
215 |
NM_080364.3 |
CG5461-RA, transcript variant A (bun), mRNA |
| 1.03 |
5 |
28 |
75 |
197 |
NM_001042894.1 |
CG5461-RF, transcript variant F (bun), mRNA |
| 1.03 |
5 |
26 |
75 |
213 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 1.03 |
5 |
24 |
83 |
168 |
NM_167000.1 |
CG32778-RA (CG32778), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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