|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8470R-4 |
| Symbol |
mRpS30 |
Full Name |
mitochondrial ribosomal protein S30 |
| CG No |
CG8470 |
Old CG No |
CG8470 |
| Synonyms |
mRSp30, MRP-S30, CG8470, mRpS30 |
| Accession No (Link to NCBI) |
NM_078612.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kim MJ, Choe KM.
Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
PLoS Genet (2014) 10(10) e1004683
[
PubMed ID = 25329560
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTTGAAGCTTAACCGTGTTCG |
| Primer Seq. 3' |
aatctagaggtaccCAACGGGGTTCGATTCGGCAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GTTGAAGCTT AACCGTGTTC GCCATCTGCG AACGACGTAC AAGCGCTGTT TGTCCGGCCA
0061 GTCGCAGCTG CAGCTGAATA GTACGGACGC AGAGCCGGCA TATCCGGAGA TTCAGGATCT
0121 GTCCTTCAAG GCGCGCAAGC AGAAGGATGC GGCCAACTGG CACGAGGAAA TCCGGCAGGT
0181 GCCGACCGGT GGAGGAGAAG ATGATCAAGA TCAATATGCC CCGCTATTAC GGCTTCAAGG
0241 TGGTCGACTT CAACGACTCG AAAATCCCCT ACAATGCACT GCCGCTCACG CAACATTATA
0301 CGCGCACAGT GCTCGAGGAT TTGCCATCTG AAACGGATAA GAAGGCACAG GCTAAGGACT
0361 CCGAGCAGGA GCAGGAGCAG CCGAGCGTGG ATGGTCTTTT CAAGGCGGCT CGCGGGGATG
0421 TTATTGATGC CCTCGAGTTT GCCCACGACT ACTACAAGCA TCTGGAAAAG CAACACAACT
0481 CTGCCGAATC GAACCCCGTT G |
| in silico PCR Fragment |
0001 GTTGAAGCTT AACCGTGTTC GCCATCTGCG AACGACGTAC AAGCGCTGTT TGTCCGGCCA
0061 GTCGCAGCTG CAGCTGAATA GTACGGACGC AGAGCCGGCA TATCCGGAGA TTCAGGATCT
0121 GTCCTTCAAG GCGCGCAAGC AGAAGGATGC GGCCAACTGG CACGAGGAAA TCCGGCAGGT
0181 GCCGA-CGGT GGAGGAGAAG ATGATCAAGA TCAATATGCC CCGCTATTAC GGCTTCAAGG
0241 TGGTCGACTT CAACGACTCG AAAATCCCCT ACAATGCACT GCCGCTCACG CAACATTATA
0301 CGCGCACAGT GCTCGAGGAT TTGCCATCTG AAACGGATAA GAAGGCACAG GCTAAGGACT
0361 CCGAGCAGGA GCAGGAGCAG CCGAGCGTGG ATGGTCTTTT CAAGGCGGCT CGCGGGGATG
0421 TTATTGATGC CCTCGAGTTT GCCCACGACT ACTACAAGCA TCTGGAAAAG CAACACAACT
0481 CTGCCGAATC GAACCCCGTT G |
| Assemble Data |
8470R-4.IR_full 1 GTTGAAGCTTAACCGTGTTCGCCATCTGCGAACGACGTACAAGCGCTGTTTGTCCGGCCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 1 GTTGAAGCTTAACCGTGTTCGCCATCTGCGAACGACGTACAAGCGCTGTTTGTCCGGCCA 60
8470R-4.IR_full 61 GTCGCAGCTGCAGCTGAATAGTACGGACGCAGAGCCGGCATATCCGGAGATTCAGGATCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 61 GTCGCAGCTGCAGCTGAATAGTACGGACGCAGAGCCGGCATATCCGGAGATTCAGGATCT 120
8470R-4.IR_full 121 GTCCTTCAAGGCGCGCAAGCAGAAGGATGCGGCCAACTGGCACGAGGAAATCCGGCAGGT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 121 GTCCTTCAAGGCGCGCAAGCAGAAGGATGCGGCCAACTGGCACGAGGAAATCCGGCAGGT 180
8470R-4.IR_full 181 GCCGACCGGTGGAGGAGAAGATGATCAAGATCAATATGCCCCGCTATTACGGCTTCAAGG 240
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 181 GCCGA-CGGTGGAGGAGAAGATGATCAAGATCAATATGCCCCGCTATTACGGCTTCAAGG 240
8470R-4.IR_full 241 TGGTCGACTTCAACGACTCGAAAATCCCCTACAATGCACTGCCGCTCACGCAACATTATA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 241 TGGTCGACTTCAACGACTCGAAAATCCCCTACAATGCACTGCCGCTCACGCAACATTATA 300
8470R-4.IR_full 301 CGCGCACAGTGCTCGAGGATTTGCCATCTGAAACGGATAAGAAGGCACAGGCTAAGGACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 301 CGCGCACAGTGCTCGAGGATTTGCCATCTGAAACGGATAAGAAGGCACAGGCTAAGGACT 360
8470R-4.IR_full 361 CCGAGCAGGAGCAGGAGCAGCCGAGCGTGGATGGTCTTTTCAAGGCGGCTCGCGGGGATG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 361 CCGAGCAGGAGCAGGAGCAGCCGAGCGTGGATGGTCTTTTCAAGGCGGCTCGCGGGGATG 420
8470R-4.IR_full 421 TTATTGATGCCCTCGAGTTTGCCCACGACTACTACAAGCATCTGGAAAAGCAACACAACT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8470R-4.in silico 421 TTATTGATGCCCTCGAGTTTGCCCACGACTACTACAAGCATCTGGAAAAGCAACACAACT 480
8470R-4.IR_full 481 CTGCCGAATCGAACCCCGTTG 501
|||||||||||||||||||||
8470R-4.in silico 481 CTGCCGAATCGAACCCCGTTG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_078612.2 |
CG8470-RA (mRpS30), mRNA |
| 0.2 |
1 |
5 |
7 |
12 |
NM_136732.1 |
CG12904-RA (CG12904), mRNA |
| 0.2 |
1 |
2 |
4 |
16 |
NM_132606.1 |
CG3754-RA (CG3754), mRNA |
| 0 |
0 |
6 |
19 |
47 |
NM_133159.1 |
CG14200-RA (CG14200), mRNA |
| 0 |
0 |
5 |
7 |
8 |
NM_137836.1 |
CG12489-RA (Dnr1), mRNA |
| 0 |
0 |
5 |
4 |
4 |
NM_135559.2 |
CG5337-RA (CG5337), mRNA |
| 0 |
0 |
3 |
9 |
13 |
NM_132521.2 |
CG1578-RA (CG1578), mRNA |
| 0 |
0 |
3 |
4 |
8 |
NM_078603.2 |
CG10952-RA, transcript variant A (eag), mRNA |
| 0 |
0 |
3 |
4 |
8 |
NM_001042810.1 |
CG10952-RB, transcript variant B (eag), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_135124.1 |
CG14001-RA (bchs), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_080356.2 |
CG5993-RA (os), mRNA |
| 0 |
0 |
3 |
2 |
15 |
NM_170450.1 |
CG31036-RA (CG31036), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_168368.1 |
CG32044-RA (CG32044), mRNA |
| 0 |
0 |
2 |
7 |
20 |
NM_144453.2 |
CG13061-RA (Nplp3), mRNA |
| 0 |
0 |
2 |
7 |
13 |
NM_132392.1 |
CG12641-RA (CG12641), mRNA |
| 0 |
0 |
2 |
7 |
7 |
NM_001038748.1 |
CG33962-RA (Cp7Fa), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_137534.1 |
CG15082-RA (CG15082), mRNA |
| 0 |
0 |
2 |
5 |
16 |
NM_169451.1 |
CG31361-RA, transcript variant A (dpr17), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_169452.1 |
CG31361-RB, transcript variant B (dpr17), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_164645.1 |
CG31915-RA (CG31915), mRNA |
| 0 |
0 |
2 |
4 |
23 |
NM_079002.2 |
CG3905-RA (Su(z)2), mRNA |
| 0 |
0 |
2 |
4 |
11 |
NM_140112.1 |
CG14165-RA (CG14165), mRNA |
| 0 |
0 |
2 |
4 |
11 |
NM_001038758.1 |
CG9908-RB, transcript variant B (disco), mRNA |
| 0 |
0 |
2 |
4 |
11 |
NM_078638.2 |
CG9908-RA, transcript variant A (disco), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_136882.2 |
CG30040-RA (jeb), mRNA |
| 0 |
0 |
2 |
3 |
17 |
NM_134505.1 |
CG14230-RA (CG14230), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_133052.1 |
CG15058-RA (CG15058), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143325.2 |
CG12876-RA (CG12876), mRNA |
| 0 |
0 |
2 |
2 |
28 |
NM_079671.2 |
CG7847-RA, transcript variant A (sr), mRNA |
| 0 |
0 |
2 |
2 |
14 |
NM_078606.3 |
CG6146-RA, transcript variant A (Top1), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
|
|
|
|
