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|
| NIG-RNAi Strains Detail |
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8439R-4 |
| Symbol |
Cct5 |
Full Name |
T-complex Chaperonin 5 |
| CG No |
CG8439 |
Old CG No |
CG8439 |
| Synonyms |
Cct5, l(2)06444, BcDNA.GM12270, CG8439, BcDNA:GM12270 |
| Accession No (Link to NCBI) |
NM_078983.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Pavel M, Imarisio S, Menzies FM, Jimenez-Sanchez M, Siddiqi FH, Wu X, Renna M, O'Kane CJ, Crowther DC, Rubinsztein DC.<br> CCT complex restricts neuropathogenic protein aggregation via autophagy.<br> Nat Commun (2016) 7 13821
[
PubMed ID = 27929117
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGAGCGCCTTTCCCGGTACG |
| Primer Seq. 3' |
aatctagaggtaccCCTTGTTCTTCGGGTCCACGG |
| Predicted Fragment Size |
490 |
| IR fragment full Seq |
0001 ATGAGNCGCC TTTCCCNGGT ACGTTCGCGT TCGACGAATA TGGTCGGCCT TTCATCATTC
0061 CTGCGCGACC CAGGAGCGCC AGAAGCGCAT CACAGGAACA GATGCCATAA AGACGCACAT
0121 TATGGCCGCC CGCCAGATCG CGTCCACCCT GAAGACCTCT CTGGGTCCCA AGGGTCTGGA
0181 CAAGATCATG GTCAGTCCCG ACGGCGACGT GACTGTGACC AACGATGGCG CCACCATCAT
0241 GAAGCTGATG GAGGTTGACC ACGAGATTGC CAAGCTGATG GTNGCAgCTG TCGCAGTCAC
0301 AGGACGACGA GATCGGCGAT GGAACCACCG GCGTTGTCGT GCTGGCCGGA GCACTCTTGG
0361 AGCAGGCCGA GGGACTGATC GACAGGGGCA TTCATCCCAT TCGCAtNGNC GATGGCTTTG
0421 AATTGGCCGC CCAGTGCGCC ATCAAGCANC TGGACGcGAT CGCCCAACCC TTCCCCGTGG
0481 ACCCGAAGAA CAAGG |
| in silico PCR Fragment |
0001 ATGAG-CGCC TTTCCC-GGT ACGTTCGCGT TCGACGAATA TGGTCGGCCT TTCATCA-TC
0061 CTGCGCGA-C CAGGAGCGCC AGAAGCGCAT CACAGGAACA GATGCCATAA AGACGCACAT
0121 TATGGCCGCC CGCCAGATCG CGTCCACCCT GAAGACCTCT CTGGGTCCCA AGGGTCTGGA
0181 CAAGATCATG GTCAGTCCCG ACGGCGACGT GACTGTGACC AACGATGGCG CCACCATCAT
0241 GAAGCTGATG GAGGTTGACC ACGAGATTGC CAAGCTGATG GT-GCAGCTG TCGCAGTCAC
0301 AGGACGACGA GATCGGCGAT GGAACCACCG GCGTTGTCGT GCTGGCCGGA GCACTCTTGG
0361 AGCAGGCCGA GGGACTGATC GACAGGGGCA TTCATCCCAT TCGCATCGCC GATGGCTTTG
0421 AATTGGCCGC CCAGTGCGCC ATCAAGCAGC TGGACGCGAT CGCCCAACCC TTCCCCGTGG
0481 ACCCGAAGAA CAAGG |
| Assemble Data |
<PRE>
8439R-4.IR_full 1 ATGAG<span class="snp"><tt>N</tt></span>CGCCTTTCCC<span class="snp"><tt>N</tt></span>GGTACGTTCGCGTTCGACGAATATGGTCGGCCTTTCATCA<span class="snp"><tt>T</tt></span>TC 60
|||||<span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
8439R-4.in silico 1 ATGAG<span class="snp"><tt>-</tt></span>CGCCTTTCCC<span class="snp"><tt>-</tt></span>GGTACGTTCGCGTTCGACGAATATGGTCGGCCTTTCATCA<span class="snp"><tt>-</tt></span>TC 60
8439R-4.IR_full 61 CTGCGCGA<span class="snp"><tt>C</tt></span>CCAGGAGCGCCAGAAGCGCATCACAGGAACAGATGCCATAAAGACGCACAT 120
||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
8439R-4.in silico 61 CTGCGCGA<span class="snp"><tt>-</tt></span>CCAGGAGCGCCAGAAGCGCATCACAGGAACAGATGCCATAAAGACGCACAT 120
8439R-4.IR_full 121 TATGGCCGCCCGCCAGATCGCGTCCACCCTGAAGACCTCTCTGGGTCCCAAGGGTCTGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8439R-4.in silico 121 TATGGCCGCCCGCCAGATCGCGTCCACCCTGAAGACCTCTCTGGGTCCCAAGGGTCTGGA 180
8439R-4.IR_full 181 CAAGATCATGGTCAGTCCCGACGGCGACGTGACTGTGACCAACGATGGCGCCACCATCAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8439R-4.in silico 181 CAAGATCATGGTCAGTCCCGACGGCGACGTGACTGTGACCAACGATGGCGCCACCATCAT 240
8439R-4.IR_full 241 GAAGCTGATGGAGGTTGACCACGAGATTGCCAAGCTGATGGT<span class="snp"><tt>N</tt></span>GCAGCTGTCGCAGTCAC 300
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
8439R-4.in silico 241 GAAGCTGATGGAGGTTGACCACGAGATTGCCAAGCTGATGGT<span class="snp"><tt>-</tt></span>GCAGCTGTCGCAGTCAC 300
8439R-4.IR_full 301 AGGACGACGAGATCGGCGATGGAACCACCGGCGTTGTCGTGCTGGCCGGAGCACTCTTGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8439R-4.in silico 301 AGGACGACGAGATCGGCGATGGAACCACCGGCGTTGTCGTGCTGGCCGGAGCACTCTTGG 360
8439R-4.IR_full 361 AGCAGGCCGAGGGACTGATCGACAGGGGCATTCATCCCATTCGCAT<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>N</tt></span>CGATGGCTTTG 420
||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|||||||||||
8439R-4.in silico 361 AGCAGGCCGAGGGACTGATCGACAGGGGCATTCATCCCATTCGCAT<span class="snp"><tt>C</tt></span>G<span class="snp"><tt>C</tt></span>CGATGGCTTTG 420
8439R-4.IR_full 421 AATTGGCCGCCCAGTGCGCCATCAAGCA<span class="snp"><tt>N</tt></span>CTGGACGCGATCGCCCAACCCTTCCCCGTGG 480
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||
8439R-4.in silico 421 AATTGGCCGCCCAGTGCGCCATCAAGCA<span class="snp"><tt>G</tt></span>CTGGACGCGATCGCCCAACCCTTCCCCGTGG 480
8439R-4.IR_full 481 ACCCGAAGAACAAGG 495
|||||||||||||||
8439R-4.in silico 481 ACCCGAAGAACAAGG 495
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
472 |
0 |
0 |
1 |
NM_078983.2 |
CG8439-RA, transcript variant A (Cct5), mRNA |
| 77.33 |
365 |
1 |
2 |
2 |
NM_165867.1 |
CG8439-RB, transcript variant B (Cct5), mRNA |
| 0.63 |
3 |
9 |
6 |
16 |
NM_135735.1 |
CG5525-RA (CG5525), mRNA |
| 0 |
0 |
17 |
3 |
9 |
NM_141578.1 |
CG8351-RA (CG8351), mRNA |
| 0 |
0 |
5 |
16 |
30 |
NM_170016.1 |
CG5374-RB, transcript variant B (T-cp1), mRNA |
| 0 |
0 |
5 |
16 |
30 |
NM_079726.3 |
CG5374-RA, transcript variant A (T-cp1), mRNA |
| 0 |
0 |
5 |
16 |
8 |
NM_167176.1 |
CG7033-RB, transcript variant B (CG7033), mRNA |
| 0 |
0 |
5 |
16 |
8 |
NM_132296.2 |
CG7033-RA, transcript variant A (CG7033), mRNA |
| 0 |
0 |
5 |
16 |
8 |
NM_176715.1 |
CG7033-RC, transcript variant C (CG7033), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_138052.2 |
CG3356-RA (CG3356), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_170557.1 |
CG11525-RD, transcript variant D (CycG), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_170556.1 |
CG11525-RC, transcript variant C (CycG), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_170558.1 |
CG11525-RE, transcript variant E (CycG), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_079870.2 |
CG11525-RA, transcript variant A (CycG), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_170555.1 |
CG11525-RB, transcript variant B (CycG), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132765.1 |
CG5548-RA (CG5548), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132888.1 |
CG13957-RA (CG13957), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_206711.1 |
CG33249-RA (CG33249), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079277.2 |
CG3705-RA (aay), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001042796.1 |
CG6775-RC, transcript variant C (rg), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080023.1 |
CG6775-RA, transcript variant A (rg), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167028.1 |
CG6775-RB, transcript variant B (rg), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_079821.2 |
CG1954-RA (Pkc98E), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_143284.2 |
CG6059-RA (CG6059), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_078867.2 |
CG5526-RA (Dhc36C), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137163.3 |
CG10255-RA (Lap1), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169806.1 |
CG31235-RA (CG31235), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141399.1 |
CG9727-RA (CG9727), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167135.2 |
CG10944-RC, transcript variant C (RpS6), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167136.1 |
CG10944-RA, transcript variant A (RpS6), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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