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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8308R-3 |
| Symbol |
alphaTub67C |
Full Name |
alpha-Tubulin at 67C |
| CG No |
CG8308 |
Old CG No |
CG8308 |
| Synonyms |
alpha-tubulin, alpha-Tub, alphaTUB, mum-tub67C, alpha-tub67C, CG8308, ALPHA 67C, D.m.ALPHA-67C, alphaTub[67C], alphatub67C, alpha4, aTub67C, alpha4Tub67C, alpha4-t, Tom, T, 4t, Fs(3)Tomaj, alpha67C, DTA2, alphaTub67C, Fs(3)Tom, Fs(3)Sz22, alphaTub4, alpha4t, alpha-Tub67C, alphaTub, alphatub, Tub |
| Accession No (Link to NCBI) |
NM_079285.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.<br> The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in <i>Drosophila</i>.<br> Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCGGCAATGCCTGCTGGGAG |
| Primer Seq. 3' |
aatctagaggtaccACGGCAAAGTCCAGCTTGCAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATCGGCAATG CCTGCTGGGA GCTGTACCTG CTGGAGCACG GCATCaACCT GGACGGCAGC
0061 CTGAAGACCA AGGAGGAGCT GACGGCCAGC GGGAGCAGTG CCAGTGTGGG TCACGACACC
0121 TCGGCCAACG ATGCTAGGAC CTTCTTCACG GAGACCGGCA ATGGAAAACA AGTGCCACGC
0181 TCGATTTTCG TCGATCTGGA ACCGACGGTC ATCGATGATG TTCGGAATGG CTGCATGAGG
0241 GAGCTCTATC ATCCGGAGCA ACTGATTTCT GGAAAGGAAG ATGCGGCCAA TAACTATGCC
0301 CGAGGTCGTT ACTCCATCTG GCAAGGAGGT GATCGATAGG GTGAcCTCAC GGCTGCAGAA
0361 GATCGCAGAG CAGTGTGACA GCTTGCAGGG ATTCCTCCAT CTTCCACTCG CTGGGCGGTG
0421 GTACTGGTTC gGGATTCACA TCCCTGTTGG TGGAGCGCTT GTCCACCGAT TACAGCAAGA
0481 AGTGCAAGCT GGACTTTGCC GT |
| in silico PCR Fragment |
0001 ATCGGCAATG CCTGCTGGGA GCTGTACCTG CTGGAGCACG GCATCAACCT GGACGGCAGC
0061 CTGAAGACCA AGGAGGAGCT GACGGCCAGC GGGAGCAGTG CCAGTGTGGG TCACGACACC
0121 TCGGCCAACG ATGCTAGGAC CTTCTTCACG GAGACCGGCA ATGGAAAACA AGTGCCACGC
0181 TCGATTTTCG TCGATCTGGA ACCGACGGTC ATCGATGATG TTCGGAATGG CTGCATGAGG
0241 GAGCTCTATC ATCCGGAGCA ACTGATTTCT GGAAAGGAAG ATGCGGCCAA TAACTATGCC
0301 CGAGGTCGTT ACTCCAT-TG GCAAGGAGGT GATCGATAGG GTGACCTCAC GGCTGCAGAA
0361 GATCGCAGAG CAGTGTGACA GCTTGCAGGG ATTCCT-CAT CTTCCACTCG CTGGGCGGTG
0421 GTACTGGTTC GGGATTCACA TCCCTGTTGG TGGAGCGCTT GTCCACCGAT TACAGCAAGA
0481 AGTGCAAGCT GGACTTTGCC GT |
| Assemble Data |
<PRE>
8308R-3.IR_full 1 ATCGGCAATGCCTGCTGGGAGCTGTACCTGCTGGAGCACGGCATCAACCTGGACGGCAGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 1 ATCGGCAATGCCTGCTGGGAGCTGTACCTGCTGGAGCACGGCATCAACCTGGACGGCAGC 60
8308R-3.IR_full 61 CTGAAGACCAAGGAGGAGCTGACGGCCAGCGGGAGCAGTGCCAGTGTGGGTCACGACACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 61 CTGAAGACCAAGGAGGAGCTGACGGCCAGCGGGAGCAGTGCCAGTGTGGGTCACGACACC 120
8308R-3.IR_full 121 TCGGCCAACGATGCTAGGACCTTCTTCACGGAGACCGGCAATGGAAAACAAGTGCCACGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 121 TCGGCCAACGATGCTAGGACCTTCTTCACGGAGACCGGCAATGGAAAACAAGTGCCACGC 180
8308R-3.IR_full 181 TCGATTTTCGTCGATCTGGAACCGACGGTCATCGATGATGTTCGGAATGGCTGCATGAGG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 181 TCGATTTTCGTCGATCTGGAACCGACGGTCATCGATGATGTTCGGAATGGCTGCATGAGG 240
8308R-3.IR_full 241 GAGCTCTATCATCCGGAGCAACTGATTTCTGGAAAGGAAGATGCGGCCAATAACTATGCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 241 GAGCTCTATCATCCGGAGCAACTGATTTCTGGAAAGGAAGATGCGGCCAATAACTATGCC 300
8308R-3.IR_full 301 CGAGGTCGTTACTCCAT<span class="snp"><tt>C</tt></span>TGGCAAGGAGGTGATCGATAGGGTGACCTCACGGCTGCAGAA 360
|||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 301 CGAGGTCGTTACTCCAT<span class="snp"><tt>-</tt></span>TGGCAAGGAGGTGATCGATAGGGTGACCTCACGGCTGCAGAA 360
8308R-3.IR_full 361 GATCGCAGAGCAGTGTGACAGCTTGCAGGGATTCCT<span class="snp"><tt>C</tt></span>CATCTTCCACTCGCTGGGCGGTG 420
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
8308R-3.in silico 361 GATCGCAGAGCAGTGTGACAGCTTGCAGGGATTCCT<span class="snp"><tt>-</tt></span>CATCTTCCACTCGCTGGGCGGTG 420
8308R-3.IR_full 421 GTACTGGTTCGGGATTCACATCCCTGTTGGTGGAGCGCTTGTCCACCGATTACAGCAAGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8308R-3.in silico 421 GTACTGGTTCGGGATTCACATCCCTGTTGGTGGAGCGCTTGTCCACCGATTACAGCAAGA 480
8308R-3.IR_full 481 AGTGCAAGCTGGACTTTGCCGT 502
||||||||||||||||||||||
8308R-3.in silico 481 AGTGCAAGCTGGACTTTGCCGT 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079285.2 |
CG8308-RA (alphaTub67C), mRNA |
| 0.41 |
2 |
8 |
28 |
42 |
NM_079573.3 |
CG9476-RA (alphaTub85E), mRNA |
| 0.41 |
2 |
3 |
3 |
6 |
NM_132413.1 |
CG15295-RA (CG15295), mRNA |
| 0.2 |
1 |
2 |
4 |
4 |
NM_078772.2 |
CG13772-RA (neuroligin), mRNA |
| 0 |
0 |
15 |
32 |
37 |
NM_169189.1 |
CG2512-RB, transcript variant B (alphaTub84D), mRNA |
| 0 |
0 |
15 |
32 |
37 |
NM_079540.4 |
CG2512-RA, transcript variant A (alphaTub84D), mRNA |
| 0 |
0 |
11 |
31 |
38 |
NM_057424.3 |
CG1913-RA (alphaTub84B), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_176164.1 |
CG33182-RA (CG33182), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_135787.2 |
CG9414-RA (Rep4), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001032010.1 |
CG6783-RA, transcript variant A (CG6783), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164929.1 |
CG6206-RB, transcript variant B (CG6206), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135564.3 |
CG6206-RA, transcript variant A (CG6206), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001032009.1 |
CG6783-RB, transcript variant B (CG6783), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001032008.1 |
CG6783-RC, transcript variant C (CG6783), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001032005.1 |
CG6782-RD, transcript variant D (CG6782), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080218.2 |
CG8137-RA (Spn2), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135917.2 |
CG18480-RA (CG18480), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135608.1 |
CG6724-RA (CG6724), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_001032264.1 |
CG4844-RB, transcript variant B (CG4844), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_001032266.1 |
CG18431-RA, transcript variant A (CG18431), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_001032265.1 |
CG4844-RA, transcript variant A (CG4844), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_136077.1 |
CG10623-RA (CG10623), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139720.2 |
CG5150-RA (CG5150), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_132615.1 |
CG4395-RA (CG4395), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_057378.2 |
CG4200-RA (sl), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_170236.1 |
CG31096-RA (Lgr3), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_168246.1 |
CG8042-RB, transcript variant B (CG8042), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_139908.2 |
CG8042-RA, transcript variant A (CG8042), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_139477.1 |
CG14949-RA (CG14949), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167996.1 |
CG32278-RA (CG32278), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
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|
