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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8222R-2 
 Symbol Pvr  Full Name PDGF- and VEGF-receptor related 
 CG No CG8222  Old CG No CG8222 
 Synonyms VEGFR, PVR, pvr, CG8222, VEGFR-A, Vegfr-c, Vegfr-b, stai, Vegfr, DmVEGFR, vgr1, VGR1, CT24332, Vgr1, Pvr 
 Accession No (Link to NCBI) NM_078785.2 
 Inserted Chr.
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Sansone CL, Cohen J, Yasunaga A, Xu J, Osborn G, Subramanian H, Gold B, Buchon N, Cherry S.
Microbiota-Dependent Priming of Antiviral Intestinal Immunity in Drosophila.
Cell Host Microbe (2015) 18(5) 571-81 [ PubMed ID = 26567510 ] [ RRC reference ]

Garlena RA, Lennox AL, Baker LR, Parsons TE, Weinberg SM, Stronach BE.
The receptor tyrosine kinase Pvr promotes tissue closure by coordinating corpse removal and epidermal zippering.
Development (2015) 142(19) 3403-15 [ PubMed ID = 26293306 ] [ RRC reference ]

Tsuzuki S, Matsumoto H, Furihata S, Ryuda M, Tanaka H, Sung EJ, Bird GS, Zhou Y, Shears SB, Hayakawa Y.
Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP.
Nat Commun (2014) 5 4628 [ PubMed ID = 25130174 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H.
PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis.
EMBO J. (2004) 23(20) 3984-94 [ PubMed ID = 15457211 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgTGCTTCCGCGGTTGATTCTGC 
 Primer Seq. 3'
aatctagaggtaccTGCCGTCACCACGTTATGACT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 TGCTTCCGCG GTTGATTCTG CTGCCACTGC TCCTGATTTT GCGGATCTCG TGGAGCGATG 
0061 CTGTgNCTTT GCAGCAGTTC TCACCGGATC CCGATGACAG CATCGAGAAC TGCGGCGGCG 
0121 AAAATGGAGC TCCCCTGATG ACGCCCTGCA AGAGCGCCAT TATCCCTCGA TGCCCAGACG 
0181 AGCACCCACG CTAAAGTGCG AGGACGACGA GCCGATGAGC TGGTGGACCA GTCAATCGCA 
0241 ATATGTGCAT GTAAAGTCCT TCGATAATAC GGAGGATCCG GCTCGACCAT TCGGAACTAG 
0301 CCTGCATCTC ATCGAAGTGA CGGCTGACTA TGTGGCAGCC TACTATTGCG TGAAGACTTC 
0361 GAAATTCAGT CAGATCGCCA AGGAGGAGCA GTCGGACGAG GCGATGATCG AATTGGTTAA 
0421 TCAAGGATAC GCCAGCTCCA TCTACGTGTA CGTGAATGAT CCGGACACTA AGCTGGTCGA 
0481 TAGTCATAAC GTGGTGACGG CA 
 in silico PCR Fragment
0001 TGCTTCCGCG GTTGATTCTG CTGCCACTGC TCCTGATTTT GCGGATCTCG TGGAGCGATG 
0061 CTGTGCCTTT GCAGCAGTTC TCACCGGATC CCGATGACAG CATCGAGAAC TGCGGCGGCG 
0121 AGAATGGAGC TCCCCTGATG ACGCCCTGCA AGAGCGCCAT TAT-CCTGGA TGCCCAGACG 
0181 AGCA-CCACG CTTAAGTGCG AGGACGACGA GCCGATGAGC TGGTGGACCA GTCAATCGCA 
0241 ATATGTGCAT GTGAAGTCCT TCGATAATAC GGAGGATCCG GCTCGACCAT TCGGAACTAG 
0301 CCTGCATCTC ATCGAAGTGA CGGCTGACTA TGTGGCAGCC TACTATTGCG TGAAGACTTC 
0361 GAAATTCAGT CAGATCGCCA AGGAGGAGCA GTCGGACGAG GCGATGATCG AATTGGTTAA 
0421 TCAAGGATAC GCCAGCTCCA TCTACGTGTA CGTGAATGAT CCGGACACTA AGCTGGTCGA 
0481 TAGTCATAAC GTGGTGACGG CA 
 Assemble Data

8222R-2.IR_full       1   TGCTTCCGCGGTTGATTCTGCTGCCACTGCTCCTGATTTTGCGGATCTCGTGGAGCGATG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     1   TGCTTCCGCGGTTGATTCTGCTGCCACTGCTCCTGATTTTGCGGATCTCGTGGAGCGATG 60


8222R-2.IR_full       61  CTGTGNCTTTGCAGCAGTTCTCACCGGATCCCGATGACAGCATCGAGAACTGCGGCGGCG 120
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     61  CTGTGCCTTTGCAGCAGTTCTCACCGGATCCCGATGACAGCATCGAGAACTGCGGCGGCG 120


8222R-2.IR_full       121 AAAATGGAGCTCCCCTGATGACGCCCTGCAAGAGCGCCATTATCCCTCGATGCCCAGACG 180
                          | ||||||||||||||||||||||||||||||||||||||||| ||| ||||||||||||
8222R-2.in silico     121 AGAATGGAGCTCCCCTGATGACGCCCTGCAAGAGCGCCATTAT-CCTGGATGCCCAGACG 180


8222R-2.IR_full       181 AGCACCCACGCTAAAGTGCGAGGACGACGAGCCGATGAGCTGGTGGACCAGTCAATCGCA 240
                          |||| ||||||| |||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     181 AGCA-CCACGCTTAAGTGCGAGGACGACGAGCCGATGAGCTGGTGGACCAGTCAATCGCA 240


8222R-2.IR_full       241 ATATGTGCATGTAAAGTCCTTCGATAATACGGAGGATCCGGCTCGACCATTCGGAACTAG 300
                          |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     241 ATATGTGCATGTGAAGTCCTTCGATAATACGGAGGATCCGGCTCGACCATTCGGAACTAG 300


8222R-2.IR_full       301 CCTGCATCTCATCGAAGTGACGGCTGACTATGTGGCAGCCTACTATTGCGTGAAGACTTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     301 CCTGCATCTCATCGAAGTGACGGCTGACTATGTGGCAGCCTACTATTGCGTGAAGACTTC 360


8222R-2.IR_full       361 GAAATTCAGTCAGATCGCCAAGGAGGAGCAGTCGGACGAGGCGATGATCGAATTGGTTAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     361 GAAATTCAGTCAGATCGCCAAGGAGGAGCAGTCGGACGAGGCGATGATCGAATTGGTTAA 420


8222R-2.IR_full       421 TCAAGGATACGCCAGCTCCATCTACGTGTACGTGAATGATCCGGACACTAAGCTGGTCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-2.in silico     421 TCAAGGATACGCCAGCTCCATCTACGTGTACGTGAATGATCCGGACACTAAGCTGGTCGA 480


8222R-2.IR_full       481 TAGTCATAACGTGGTGACGGCA 502
                          ||||||||||||||||||||||
8222R-2.in silico     481 TAGTCATAACGTGGTGACGGCA 502

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_205927.1  CG8222-RD, transcript variant D (Pvr), mRNA 
100   482  NM_164801.1  CG8222-RB, transcript variant B (Pvr), mRNA 
100   482  NM_078785.2  CG8222-RA, transcript variant A (Pvr), mRNA 
100   482  NM_205925.1  CG8222-RF, transcript variant F (Pvr), mRNA 
100   482  NM_205926.1  CG8222-RE, transcript variant E (Pvr), mRNA 
86.92   419  NM_175983.1  CG8222-RC, transcript variant C (Pvr), mRNA 
0   NM_057631.4  CG5786-RA (ppan), mRNA 
0   NM_001043216.1  CG34113-RO, transcript variant O (CG34113), mRNA 
0   NM_001043217.1  CG34113-RP, transcript variant P (CG34113), mRNA 
0   NM_142463.2  CG7678-RA (CG7678), mRNA 
0   NM_140132.3  CG32056-RA, transcript variant A (CG32056), mRNA 
0   NM_168404.1  CG32056-RB, transcript variant B (CG32056), mRNA 
0   NM_168403.1  CG32056-RC, transcript variant C (CG32056), mRNA 
0   NM_132792.2  CG6227-RA (CG6227), mRNA 
0   NM_079833.2  CG7917-RA (Nlp), mRNA 
0   NM_141800.1  CG14701-RA (CG14701), mRNA 
0   NM_166096.1  CG30470-RA (CG30470), mRNA 
0   NM_169482.1  CG7340-RA, transcript variant A (granny-smith), mRNA 
0   NM_169483.1  CG7340-RC, transcript variant C (granny-smith), mRNA 
0   NM_141983.2  CG7340-RB, transcript variant B (granny-smith), mRNA 
0   12  NM_170367.1  CG1866-RA, transcript variant A (Moca-cyp), mRNA 
0   12  NM_143361.3  CG1866-RB, transcript variant B (Moca-cyp), mRNA 
0   NM_080157.2  CG11648-RB, transcript variant B (Abd-B), mRNA 
0   10  NM_165794.2  CG11895-RA (stan), mRNA 
0   NM_168038.1  CG32264-RD, transcript variant D (CG32264), mRNA 
0   NM_132164.1  CG18155-RA, transcript variant A (CG18155), mRNA 
0   NM_166949.1  CG2694-RA, transcript variant A (CG2694), mRNA 
0   NM_130668.2  CG2694-RB, transcript variant B (CG2694), mRNA 
0   NM_206637.1  CG18155-RB, transcript variant B (CG18155), mRNA 
0   NM_135391.2  CG13095-RA (CG13095), mRNA 

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