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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8203R-1

Symbol Cdk5 Full Name Cyclin-dependent kinase 5

CG No CG8203 Old CG No CG8203

Synonyms CDK5, CG8203, cdk5, DmCdk5, Cdk5

Accession No (Link to NCBI) NM_057732.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Saito T, Oba T, Shimizu S, Asada A, Iijima KM, Ando K.
Cdk5 increases MARK4 activity and augments pathological tau accumulation and toxicity through tau phosphorylation at Ser262.
Hum Mol Genet (2019) 28(18) 3062-3071 [ PubMed ID = 31174206 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATGGAGAAGATCGGGGAGGG

Primer Seq. 3'

aatctagaggtaccAGGGTCACCACTTCTGCGGAG

Predicted Fragment Size

IR fragment full Seq

0001 AATGNAGAAG ATCGGGGAGG GCACCTACGG CACGGTGTTC AAGGGTCGCA ACCGCGACAC 
0061 CATGGAAATA GTGGCCCTGA AACGGGTGCG ACTGGACGAG GACGACGAGG GTGTGCCCAG 
0121 TTNCTGCCCT CCGGGAGATC TGCCTGCTGA AGGAGCTGAA GCACAAGAAC ATTGTGCGCC 
0181 NTCATAGACG TCCTGCACTC GGACAAGAAA CTCACCCTGG TCTTCGAGCA CTGCGACCAG 
0241 GACCTCAAGA AGTACTTTGA CAGCCTCAAC GGGGAGATCG ACATGGCCGT CTGCAGGAGC 
0301 TTTATGCTcC AACTGCTCCG CGGACTGGCC TTCTGCCACA GCCATAATGT CCTGCATCGC 
0361 GATCTGAAAC CACAGAACCT GCTGATCAAC AAAAACGGCG AGCTAAAGCT GGCTGACTTT 
0421 GGTCTGGCTA GAGcCTTTGG CATTCCCGTG AAGTGCTACT CCGCAGAAGT GGTGACCCT

in silico PCR Fragment

0001 AATGGAGAAG ATCGGGGAGG GCACCTACGG CACGGTGTTC AAGGGTCGCA ACCGCGACAC 
0061 CATGGAAATA GTGGCCCTGA AACGGGTGCG ACTGGACGAG GACGACGAGG GTGTGCCCAG 
0121 TT-CTGCCCT CCGGGAGATC TGCCTGCTGA AGGAGCTGAA GCACAAGAAC ATTGTGCGCC 
0181 -TCATAGACG TCCTGCACTC GGACAAGAAA CTCACCCTGG TCTTCGAGCA CTGCGACCAG 
0241 GACCTCAAGA AGTACTTTGA CAGCCTCAAC GGGGAGATCG ACATGGCCGT CTGCAGGAGC 
0301 TTTATGCTCC AACTGCTCCG CGGACTGGCA TTCTGCCACA GCCATAATGT CCTGCATCGC 
0361 GATCTGAAAC CACAGAACCT GCTGATCAAC AAAAACGGCG AGCTAAAGCT AGCTGACTTT 
0421 GGTCTCGCTA GAGCCTTTGG CATTCCCGTG AAGTGCTACT CCGCAGAAGT GGTGACCCT

Assemble Data


8203R-1.IR_full       1   AATGNAGAAGATCGGGGAGGGCACCTACGGCACGGTGTTCAAGGGTCGCAACCGCGACAC 60
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico     1   AATGGAGAAGATCGGGGAGGGCACCTACGGCACGGTGTTCAAGGGTCGCAACCGCGACAC 60


8203R-1.IR_full       61  CATGGAAATAGTGGCCCTGAAACGGGTGCGACTGGACGAGGACGACGAGGGTGTGCCCAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico     61  CATGGAAATAGTGGCCCTGAAACGGGTGCGACTGGACGAGGACGACGAGGGTGTGCCCAG 120


8203R-1.IR_full       121 TTNCTGCCCTCCGGGAGATCTGCCTGCTGAAGGAGCTGAAGCACAAGAACATTGTGCGCC 180
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico     121 TT-CTGCCCTCCGGGAGATCTGCCTGCTGAAGGAGCTGAAGCACAAGAACATTGTGCGCC 180


8203R-1.IR_full        181 TCATAGACGTCCTGCACTCGGACAAGAAACTCACCCTGGTCTTCGAGCACTGCGACCAG 239
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico      181 TCATAGACGTCCTGCACTCGGACAAGAAACTCACCCTGGTCTTCGAGCACTGCGACCAG 239


8203R-1.IR_full       241 GACCTCAAGAAGTACTTTGACAGCCTCAACGGGGAGATCGACATGGCCGTCTGCAGGAGC 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico     241 GACCTCAAGAAGTACTTTGACAGCCTCAACGGGGAGATCGACATGGCCGTCTGCAGGAGC 299


8203R-1.IR_full       301 TTTATGCTCCAACTGCTCCGCGGACTGGCCTTCTGCCACAGCCATAATGTCCTGCATCGC 359
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
8203R-1.in silico     301 TTTATGCTCCAACTGCTCCGCGGACTGGCATTCTGCCACAGCCATAATGTCCTGCATCGC 359


8203R-1.IR_full       361 GATCTGAAACCACAGAACCTGCTGATCAACAAAAACGGCGAGCTAAAGCTGGCTGACTTT 419
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
8203R-1.in silico     361 GATCTGAAACCACAGAACCTGCTGATCAACAAAAACGGCGAGCTAAAGCTAGCTGACTTT 419


8203R-1.IR_full       421 GGTCTGGCTAGAGCCTTTGGCATTCCCGTGAAGTGCTACTCCGCAGAAGTGGTGACCCT 478
                          ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
8203R-1.in silico     421 GGTCTCGCTAGAGCCTTTGGCATTCCCGTGAAGTGCTACTCCGCAGAAGTGGTGACCCT 478

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	459	0	0	0	NM_057732.3	CG8203-RA (Cdk5), mRNA
0	0	5	2	2	NM_136002.1	CG15142-RA (CG15142), mRNA
0	0	4	3	6	NM_001042817.1	CG4937-RB, transcript variant B (RhoGAP15B), mRNA
0	0	4	3	6	NM_132955.1	CG4937-RA, transcript variant A (RhoGAP15B), mRNA
0	0	4	3	2	NM_137098.2	CG8485-RA, transcript variant A (CG8485), mRNA
0	0	4	3	2	NM_166043.1	CG8485-RD, transcript variant D (CG8485), mRNA
0	0	4	3	2	NM_166041.1	CG8485-RB, transcript variant B (CG8485), mRNA
0	0	4	3	2	NM_166042.1	CG8485-RC, transcript variant C (CG8485), mRNA
0	0	4	2	7	NM_078666.2	CG8146-RA (Socs16D), mRNA
0	0	3	2	9	NM_138020.2	CG3105-RA (CG3105), mRNA
0	0	2	6	4	NM_168245.2	CG8110-RB, transcript variant B (syd), mRNA
0	0	2	6	4	NM_079913.2	CG8110-RA, transcript variant A (syd), mRNA
0	0	2	5	6	NM_168961.2	CG32451-RB, transcript variant B (SPoCk), mRNA
0	0	2	4	2	NM_132908.2	CG4453-RA (Nup153), mRNA
0	0	2	3	5	NM_169549.1	CG9351-RC, transcript variant C (flfl), mRNA
0	0	2	3	5	NM_142047.1	CG9351-RA, transcript variant A (flfl), mRNA
0	0	2	3	5	NM_169548.1	CG9351-RB, transcript variant B (flfl), mRNA
0	0	2	3	3	NM_079771.1	CG11921-RA (fd96Ca), mRNA
0	0	2	3	2	NM_079706.2	CG3412-RA (slmb), mRNA
0	0	2	2	3	NM_176111.1	CG2049-RC, transcript variant C (Pkn), mRNA
0	0	2	2	3	NM_176113.1	CG2049-RD, transcript variant D (Pkn), mRNA
0	0	1	18	35	NM_079696.4	CG10498-RB, transcript variant B (cdc2c), mRNA
0	0	1	18	35	NM_169916.1	CG10498-RA, transcript variant A (cdc2c), mRNA
0	0	1	10	10	NM_132488.3	CG11727-RB, transcript variant B (CG11727), mRNA
0	0	1	10	10	NM_167285.2	CG11727-RA, transcript variant A (CG11727), mRNA
0	0	1	6	7	NM_141068.2	CG7597-RA, transcript variant A (CG7597), mRNA
0	0	1	6	7	NM_168912.1	CG7597-RB, transcript variant B (CG7597), mRNA
0	0	1	5	10	NM_167525.1	CG9802-RB, transcript variant B (Cap), mRNA
0	0	1	5	10	NM_078650.2	CG9802-RA, transcript variant A (Cap), mRNA
0	0	1	5	3	NM_176459.1	CG17228-RD, transcript variant D (pros), mRNA

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