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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 8112R-2 
 Symbol CG8112  Full Name CG8112 
 CG No CG8112  Old CG No CG8112 
 Synonyms CG8112 
 Accession No (Link to NCBI) NM_141559.3 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Chi KC, Tsai WC, Wu CL, Lin TY, Hueng DY.<br> An Adult Drosophila Glioma Model for Studying Pathometabolic Pathways of Gliomagenesis.<br> Mol Neurobiol (2019) 56(6) 4589-4599 [ PubMed ID = 30357574 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGCGGAGGAGGAGAAAGAAG 
 Primer Seq. 3' 
aatctagaggtaccGGAACCTCGGAACACATGAGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGCGGAGGA GGAGAAAGAA GGCAGTGAGC GACCCTTTGT GCCGCCGCTG AGCTGTTACA 
0061 CGTACTTTTT GTTTGCACCC ACGCTTATCT ACCGAGACAG CTACCCGCGC ACCTCCCACA 
0121 TCCGCTGGAA GTTTGCCCTG AATCGACTGT TGGAGGTGGT GGCCATAGCC TTCCTGTACG 
0181 CGTTCATCCA TGAGCGGCAC ATCCACGAGC ACTTCGGACA GTACGGACTG GAACCAATGG 
0241 GACCCTCCCA GCTGATCCTC AAGCTGTTCG GCATGATGCT GCCCAGCGCG GTGATCTTTC 
0301 TGTGCGGCTT CTACCTAATC CTGCACTCGT GGCTGAACTT CACGTCGGAG CTGCTACGCT 
0361 TCGGTGATCG AATGTTCTAC AAGGACTGGT GGACGTCGCA CACTTACGAT GGCTACTACA 
0421 GAAACTGGAA TGTCGTCGTG CACGACTGGC TGTACGAGTA CGTGTACAAG GATATGTACA 
0481 CTCATGTGTT CCGAGGTTCC  
 in silico PCR Fragment 
0001 ATGCGGAGGA GGAGAAAGAA GGCAGTGAGC GACCCTTTGT GCCGCCGCTG AGCTGTTACA 
0061 CGTACTTTTT GTTTGCACCC ACGCTTATCT ACCGAGACAG CTACCCGCGC ACCTCCCACA 
0121 TCCGCTGGAA GTTTGCCCTG AATCGACTGT TGGAGGTGGT GGCCATAGCC TTCCTGTACG 
0181 CGTTCATCCA TGAGCGGCAC ATCCACGAGC ACTTCGGACA GTACGGACTG GAACCAATGG 
0241 GACCCTCCCA GCTGATCCTC AAGCTGTTCG GCATGATGCT GCCCAGCGCG GTGATCTTTC 
0301 TGTGCGGCTT CTACCTAATC CTGCACTCGT GGCTGAACTT CACGTCGGAG CTGCTACGCT 
0361 TCGGTGATCG AATGTTCTAC AAGGACTGGT GGACGTCGCA CACTTACGAT GGCTACTACA 
0421 GAAACTGGAA TGTCGTCGTG CACGACTGGC TGTACGAGTA CGTGTACAAG GATATGTACA 
0481 CTCATGTGTT CCGAGGTTCC  
 Assemble Data 
<PRE>

8112R-2.IR_full       1   ATGCGGAGGAGGAGAAAGAAGGCAGTGAGCGACCCTTTGTGCCGCCGCTGAGCTGTTACA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     1   ATGCGGAGGAGGAGAAAGAAGGCAGTGAGCGACCCTTTGTGCCGCCGCTGAGCTGTTACA 60


8112R-2.IR_full       61  CGTACTTTTTGTTTGCACCCACGCTTATCTACCGAGACAGCTACCCGCGCACCTCCCACA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     61  CGTACTTTTTGTTTGCACCCACGCTTATCTACCGAGACAGCTACCCGCGCACCTCCCACA 120


8112R-2.IR_full       121 TCCGCTGGAAGTTTGCCCTGAATCGACTGTTGGAGGTGGTGGCCATAGCCTTCCTGTACG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     121 TCCGCTGGAAGTTTGCCCTGAATCGACTGTTGGAGGTGGTGGCCATAGCCTTCCTGTACG 180


8112R-2.IR_full       181 CGTTCATCCATGAGCGGCACATCCACGAGCACTTCGGACAGTACGGACTGGAACCAATGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     181 CGTTCATCCATGAGCGGCACATCCACGAGCACTTCGGACAGTACGGACTGGAACCAATGG 240


8112R-2.IR_full       241 GACCCTCCCAGCTGATCCTCAAGCTGTTCGGCATGATGCTGCCCAGCGCGGTGATCTTTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     241 GACCCTCCCAGCTGATCCTCAAGCTGTTCGGCATGATGCTGCCCAGCGCGGTGATCTTTC 300


8112R-2.IR_full       301 TGTGCGGCTTCTACCTAATCCTGCACTCGTGGCTGAACTTCACGTCGGAGCTGCTACGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     301 TGTGCGGCTTCTACCTAATCCTGCACTCGTGGCTGAACTTCACGTCGGAGCTGCTACGCT 360


8112R-2.IR_full       361 TCGGTGATCGAATGTTCTACAAGGACTGGTGGACGTCGCACACTTACGATGGCTACTACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     361 TCGGTGATCGAATGTTCTACAAGGACTGGTGGACGTCGCACACTTACGATGGCTACTACA 420


8112R-2.IR_full       421 GAAACTGGAATGTCGTCGTGCACGACTGGCTGTACGAGTACGTGTACAAGGATATGTACA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8112R-2.in silico     421 GAAACTGGAATGTCGTCGTGCACGACTGGCTGTACGAGTACGTGTACAAGGATATGTACA 480


8112R-2.IR_full       481 CTCATGTGTTCCGAGGTTCC 500
                          ||||||||||||||||||||
8112R-2.in silico     481 CTCATGTGTTCCGAGGTTCC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141559.3  CG8112-RA (CG8112), mRNA 
0.2   1  2  4  3  NM_132679.1  CG12176-RA (Lig4), mRNA 
0   0  2  2  5  NM_136507.1  CG11210-RA (CG11210), mRNA 
0   0  2  2  4  NM_079725.2  CG7050-RA (Nrx-1), mRNA 
0   0  2  2  1  NM_136511.2  CG2158-RA (CG2158), mRNA 
0   0  1  4  2  NM_136963.2  CG8646-RA (CG8646), mRNA 
0   0  1  3  6  NM_166141.2  CG8421-RD, transcript variant D (Asph), mRNA 
0   0  1  3  4  NM_079033.2  CG8421-RB, transcript variant B (Asph), mRNA 
0   0  1  3  4  NM_166140.1  CG8421-RA, transcript variant A (Asph), mRNA 
0   0  1  3  4  NM_134868.1  CG15399-RA (CG15399), mRNA 
0   0  1  3  3  NM_168023.1  CG11526-RA, transcript variant A (CG11526), mRNA 
0   0  1  3  2  NM_139549.1  CG11526-RB, transcript variant B (CG11526), mRNA 
0   0  1  2  4  NM_079363.3  CG7450-RA, transcript variant A (CrebA), mRNA 
0   0  1  2  4  NM_206374.1  CG7450-RB, transcript variant B (CrebA), mRNA 
0   0  1  2  3  NM_176185.1  CG8421-RE, transcript variant E (Asph), mRNA 
0   0  1  2  3  NM_169125.1  CG31557-RA (Obp83ef), mRNA 
0   0  1  2  3  NM_078582.3  CG32659-RA (Ten-a), mRNA 
0   0  1  2  3  NM_140569.2  CG5841-RA (mib1), mRNA 
0   0  1  2  2  NM_079501.2  CG1058-RA (rpk), mRNA 
0   0  0  7  8  NM_139415.1  CG13924-RA (CG13924), mRNA 
0   0  0  7  7  NM_137871.2  CG3536-RA (CG3536), mRNA 
0   0  0  7  6  NM_176735.1  CG33206-RB, transcript variant B (l(1)G0168), mRNA 
0   0  0  7  6  NM_176734.1  CG33206-RA, transcript variant A (l(1)G0168), mRNA 
0   0  0  7  3  NM_166551.2  CG30267-RA (CG30267), mRNA 
0   0  0  5  2  NM_136776.2  CG7686-RA (CG7686), mRNA 
0   0  0  4  9  NM_164582.1  CG3054-RA, transcript variant A (l(2)k05819), mRNA 
0   0  0  4  7  NM_168413.1  CG32062-RB, transcript variant B (CG32062), mRNA 
0   0  0  4  7  NM_168412.1  CG32062-RD, transcript variant D (CG32062), mRNA 
0   0  0  4  6  NM_057949.3  CG10270-RA (D19B), mRNA 
0   0  0  4  5  NM_079707.2  CG3331-RA (e), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00100 : Steroid biosynthesis

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