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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8091R-1 |
| Symbol |
Nc |
Full Name |
Nedd2-like caspase |
| CG No |
CG8091 |
Old CG No |
CG8091 |
| Synonyms |
dronc, DRONC, Dronc, CG8091, caspase-9, anon-EST:GreesD6, anon-EST:GressD6, anon-WO0118547.313, Nc, Dronc/Casp9 |
| Accession No (Link to NCBI) |
NM_079293.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Jo J, Im SH, Babcock DT, Iyer SC, Gunawan F, Cox DN, Galko MJ.<br> Drosophila caspase activity is required independently of apoptosis to produce active TNF/Eiger during nociceptive sensitization.<br> Cell Death Dis (2017) 8(5) e2786
[
PubMed ID = 28492538
]
[
RRC reference
]
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.<br> Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.<br> Dev Biol (2017) 427(1) 61-71
[
PubMed ID = 28514643
]
[
RRC reference
]
Zhang S, Chen C, Wu C, Yang Y, Li W, Xue L.<br> The canonical Wg signaling modulates Bsk-mediated cell death in Drosophila.<br> Cell Death Dis (2015) 6(4) e1713
[
PubMed ID = 25855961
]
[
RRC reference
]
Babcock DT, Landry C, Galko MJ.<br> Cytokine signaling mediates UV-induced nociceptive sensitization in Drosophila larvae.<br> Curr Biol (2009) 19(10) 799-806
[
PubMed ID = 19375319
]
[
RRC reference
]
Suissa Y, Ziv O, Dinur T, Arama E, Gerlitz O.<br> The NAB-Brk signal bifurcates at JNK to independently induce apoptosis and compensatory proliferation.<br> J Biol Chem (2011) 286(17) 15556-64
[
PubMed ID = 21385866
]
[
RRC reference
]
Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.<br> IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.<br> Curr Biol (2006) 16(15) 1531-7
[
PubMed ID = 16887350
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.<br> Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.<br> Cell Death Differ (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAAGCCATTCAACATGGACG |
| Primer Seq. 3' |
aatctagaggtaccCAACGACTTGCTGTCCTTTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAAGCCATT CAACATGGAC GAGAAGGATG TGCGTGTGGA GCAGCATCGT AGGCTCCTAT
0061 TGAAGATCAC CCAGCGTGGT CCCACCGCCT ATAACCTGCT GATCAATGCA CTGCGCAATA
0121 TCAATTGTCT GGATGCGGCC GTTCTATTGG AATCCGTCGA TGAGTCCGAT TCAAGGCCAC
0181 CCTTTATCTC GCTAAACGAA CGGAGAACCA GCCGGAAGTC GGCCGATATT GTGGACACAC
0241 CCTCACCCGA AGCCTCCGAA GGACCCTGCG TTAGCAANCT CCGGAATGAG CCGCTGGGAG
0301 CACTCACCCC TTATGTGGGT GTCGTTGACG GTCCCGAGGT AAAAAAATCG AAAAAGATAC
0361 ATgGTGGGGA TAGTGCCATA TTGGGCACAT ATAAGATGCA ATCACGTTTC AACCGAGGCG
0421 TTTTGCTAAT GGTTAACATA ATGGACTATC CGGATCAAAA CCGTCGACGG ATCGGAGCCG
0481 AAAAGGACAG CAAGTCGTTG |
| in silico PCR Fragment |
0001 GCAAGCCATT CAACATGGAC GAGAAGGATG TGCGTGTGGA GCAGCATCGT AGGCTCCTAT
0061 TGAAGATCAC CCAGCGTGGT CCCACCGCCT ATAACCTGCT GATCAATGCA CTGCGCAATA
0121 TCAATTGTCT GGATGCGGCC GTTCTATTGG AATCCGTCGA TGAGTCCGAT TCAAGGCCAC
0181 CCTTTATCTC GCTAAACGAA CGGAGAACCA GCCGGAAGTC GGCCGATATT GTGGACACAC
0241 CCTCACCCGA AGCCTCCGAA GGACCCTGCG TTAGCAAGCT CCGGAATGAG CCGCTGGGAG
0301 CACTCACCCC TTATGTGGGT GTCGTTGACG GTCCCGAGGT AAAAAAATCG AAAAAGATAC
0361 ATGGTGGGGA TAGTGCCATA TTGGGCACAT ATAAGATGCA ATCACGTTTC AACCGAGGCG
0421 TTTTGCTAAT GGTTAACATA ATGGACTATC CGGATCAAAA CCGTCGACGG ATCGGAGCCG
0481 AAAAGGACAG CAAGTCGTTG |
| Assemble Data |
<PRE>
8091R-1.IR_full 1 GCAAGCCATTCAACATGGACGAGAAGGATGTGCGTGTGGAGCAGCATCGTAGGCTCCTAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 1 GCAAGCCATTCAACATGGACGAGAAGGATGTGCGTGTGGAGCAGCATCGTAGGCTCCTAT 60
8091R-1.IR_full 61 TGAAGATCACCCAGCGTGGTCCCACCGCCTATAACCTGCTGATCAATGCACTGCGCAATA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 61 TGAAGATCACCCAGCGTGGTCCCACCGCCTATAACCTGCTGATCAATGCACTGCGCAATA 120
8091R-1.IR_full 121 TCAATTGTCTGGATGCGGCCGTTCTATTGGAATCCGTCGATGAGTCCGATTCAAGGCCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 121 TCAATTGTCTGGATGCGGCCGTTCTATTGGAATCCGTCGATGAGTCCGATTCAAGGCCAC 180
8091R-1.IR_full 181 CCTTTATCTCGCTAAACGAACGGAGAACCAGCCGGAAGTCGGCCGATATTGTGGACACAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 181 CCTTTATCTCGCTAAACGAACGGAGAACCAGCCGGAAGTCGGCCGATATTGTGGACACAC 240
8091R-1.IR_full 241 CCTCACCCGAAGCCTCCGAAGGACCCTGCGTTAGCAA<span class="snp"><tt>N</tt></span>CTCCGGAATGAGCCGCTGGGAG 300
|||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||
8091R-1.in silico 241 CCTCACCCGAAGCCTCCGAAGGACCCTGCGTTAGCAA<span class="snp"><tt>G</tt></span>CTCCGGAATGAGCCGCTGGGAG 300
8091R-1.IR_full 301 CACTCACCCCTTATGTGGGTGTCGTTGACGGTCCCGAGGTAAAAAAATCGAAAAAGATAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 301 CACTCACCCCTTATGTGGGTGTCGTTGACGGTCCCGAGGTAAAAAAATCGAAAAAGATAC 360
8091R-1.IR_full 361 ATGGTGGGGATAGTGCCATATTGGGCACATATAAGATGCAATCACGTTTCAACCGAGGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 361 ATGGTGGGGATAGTGCCATATTGGGCACATATAAGATGCAATCACGTTTCAACCGAGGCG 420
8091R-1.IR_full 421 TTTTGCTAATGGTTAACATAATGGACTATCCGGATCAAAACCGTCGACGGATCGGAGCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8091R-1.in silico 421 TTTTGCTAATGGTTAACATAATGGACTATCCGGATCAAAACCGTCGACGGATCGGAGCCG 480
8091R-1.IR_full 481 AAAAGGACAGCAAGTCGTTG 500
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8091R-1.in silico 481 AAAAGGACAGCAAGTCGTTG 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079293.2 |
CG8091-RA (Nc), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_057512.3 |
CG3724-RA (Pgd), mRNA |
| 0 |
0 |
2 |
3 |
12 |
NM_167080.1 |
CG32742-RA (l(1)G0148), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_079319.2 |
CG4153-RA (eIF-2beta), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_143432.2 |
CG31445-RA (CG31445), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165802.1 |
CG30015-RA, transcript variant A (CG30015), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136777.2 |
CG30015-RB, transcript variant B (CG30015), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167233.1 |
CG32687-RA (CG32687), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140070.2 |
CG3424-RA, transcript variant A (path), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_168722.1 |
CG6512-RB, transcript variant B (CG6512), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_168720.1 |
CG6512-RA, transcript variant A (CG6512), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_057605.3 |
CG17907-RA (Ace), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_078875.2 |
CG10446-RA (Side), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078604.3 |
CG32592-RA (hiw), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132185.1 |
CG15325-RA (CG15325), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_165170.2 |
CG31807-RA (CG31807), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_136497.3 |
CG8726-RB, transcript variant B (CG8726), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_165580.2 |
CG8726-RA, transcript variant A (CG8726), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_144312.1 |
CG16978-RA (CG16978), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167386.1 |
CG32626-RD, transcript variant D (CG32626), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132703.2 |
CG32626-RC, transcript variant C (CG32626), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167384.1 |
CG32626-RA, transcript variant A (CG32626), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167385.1 |
CG32626-RB, transcript variant B (CG32626), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_142828.1 |
CG13842-RA (CG13842), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_205875.1 |
CG32019-RE, transcript variant E (bt), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_166790.2 |
CG32019-RA, transcript variant A (bt), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_205877.1 |
CG32019-RD, transcript variant D (bt), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_205876.1 |
CG32019-RC, transcript variant C (bt), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_166096.1 |
CG30470-RA (CG30470), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04214
:
Apoptosis - fly
dme04215
:
Apoptosis - multiple species
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