NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8070R-1

Symbol Mys45A Full Name Mystery 45A

CG No CG8070 Old CG No CG8070

Synonyms CG8070, anon-WO0118547.170, Mys45A

Accession No (Link to NCBI) NM_136611.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Park SH, Lee CW, Lee JH, Park JY, Roshandell M, Brennan CA, Choe KM.
Requirement for and polarized localization of integrin proteins during Drosophila wound closure.
Mol Biol Cell (2018) 29(18) 2137-2147 [ PubMed ID = 29995573 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCGCAGTTGCAGAACCTCATC

Primer Seq. 3'

aatctagaggtaccGACATCTTGGCTGCCTTCGGA

Predicted Fragment Size

IR fragment full Seq

0001 CCGCAGTTGC AGAACCTCAT CAAGCGGGAT CCGGAGTCGT ATAGCGATGA GTTCCACATC 
0061 CAGTACCAAC ACTTTCTTAG CTTGCTGGAA GTTTTTGCGC TGAATCCCAG CGAAGAAAAC 
0121 AAATCCCTGG ATGACATCGT CATGTTTNGT CGCCCAGGTG GCTCAGTGCT ATCCGGCCGT 
0181 CTGCGAGGAG TTCCCAAAGC GTCTTTCCGA TTTGCTGAAG AACTATGCCA CCGTTCTGGA 
0241 CCCGGCTATG CGTAACTGCT TTGTGAAAGC TCTAATCCTG CTGAGAAACA AGAACCTAGT 
0301 GCCCGCGCTG GACATACTGG AACTGTTCTT TCAGTTGCTG CGGTGTCCGG ACAAGAATCT 
0361 GCGCACCTTT CTGCAAACGC ACATTGTGAC GGACATCAAG AATATGAATG CCAAGCACAA 
0421 GGACATGAAG CTCAATTCGA GCCTGCAGGC ATTCATGTAC TCCATGCTGA AGGATGCCAA 
0481 TCCGAAGGCA GCCAAGATGT C

in silico PCR Fragment

0001 CCGCAGTTGC AGAACCTCAT CAAGCGGGAT CCGGAGTCGT ATAGCGATGA GTTCCACATC 
0061 CAGTACCAAC ACTTTCTTAG CTTGCTGGAA GTTTTTGCGC TGAATCCCAG CGAAGAAAAC 
0121 AAATCCCTGG ATGACATCGT CATGTTT-GT CGCCCAGGTG GCTCAGTGCT ATCCGGCCGT 
0181 CTGCGAGGAG TTCCCAAAGC GTCTTTCCGA TTTGCTGAAG AACTATGCCA CCGTTCTGGA 
0241 CCCGGCTATG CGTAACTGCT TTGTGAAAGC TCTAATCCTG CTGAGAAACA AGAACCTAGT 
0301 GCCCGCGCTG GACATACTGG AACTGTTCTT TCAGTTGCTG CGGTGTCCGG ACAAGAATCT 
0361 GCGCACCTTT CTGCAAACGC ACATTGTGAC GGACATCAAG AATATGAATG CCAAGCACAA 
0421 GGACATGAAG CTCAATTCGA GCCTGCAGGC ATTCATGTAC TCCATGCTGA AGGATGCCAA 
0481 TCCGAAGGCA GCCAAGATGT C

Assemble Data


8070R-1.IR_full       1   CCGCAGTTGCAGAACCTCATCAAGCGGGATCCGGAGTCGTATAGCGATGAGTTCCACATC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     1   CCGCAGTTGCAGAACCTCATCAAGCGGGATCCGGAGTCGTATAGCGATGAGTTCCACATC 60


8070R-1.IR_full       61  CAGTACCAACACTTTCTTAGCTTGCTGGAAGTTTTTGCGCTGAATCCCAGCGAAGAAAAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     61  CAGTACCAACACTTTCTTAGCTTGCTGGAAGTTTTTGCGCTGAATCCCAGCGAAGAAAAC 120


8070R-1.IR_full       121 AAATCCCTGGATGACATCGTCATGTTTNGTCGCCCAGGTGGCTCAGTGCTATCCGGCCGT 180
                          ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
8070R-1.in silico     121 AAATCCCTGGATGACATCGTCATGTTT-GTCGCCCAGGTGGCTCAGTGCTATCCGGCCGT 180


8070R-1.IR_full       181 CTGCGAGGAGTTCCCAAAGCGTCTTTCCGATTTGCTGAAGAACTATGCCACCGTTCTGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     181 CTGCGAGGAGTTCCCAAAGCGTCTTTCCGATTTGCTGAAGAACTATGCCACCGTTCTGGA 240


8070R-1.IR_full       241 CCCGGCTATGCGTAACTGCTTTGTGAAAGCTCTAATCCTGCTGAGAAACAAGAACCTAGT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     241 CCCGGCTATGCGTAACTGCTTTGTGAAAGCTCTAATCCTGCTGAGAAACAAGAACCTAGT 300


8070R-1.IR_full       301 GCCCGCGCTGGACATACTGGAACTGTTCTTTCAGTTGCTGCGGTGTCCGGACAAGAATCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     301 GCCCGCGCTGGACATACTGGAACTGTTCTTTCAGTTGCTGCGGTGTCCGGACAAGAATCT 360


8070R-1.IR_full       361 GCGCACCTTTCTGCAAACGCACATTGTGACGGACATCAAGAATATGAATGCCAAGCACAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     361 GCGCACCTTTCTGCAAACGCACATTGTGACGGACATCAAGAATATGAATGCCAAGCACAA 420


8070R-1.IR_full       421 GGACATGAAGCTCAATTCGAGCCTGCAGGCATTCATGTACTCCATGCTGAAGGATGCCAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8070R-1.in silico     421 GGACATGAAGCTCAATTCGAGCCTGCAGGCATTCATGTACTCCATGCTGAAGGATGCCAA 480


8070R-1.IR_full       481 TCCGAAGGCAGCCAAGATGTC 501
                          |||||||||||||||||||||
8070R-1.in silico     481 TCCGAAGGCAGCCAAGATGTC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136611.2	CG8070-RA (Mys45A), mRNA
0	0	2	4	5	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	2	4	4	NM_057796.2	CG10379-RA (mbc), mRNA
0	0	2	2	2	NM_142571.1	CG6300-RA (CG6300), mRNA
0	0	2	2	2	NM_142572.1	CG11659-RA (CG11659), mRNA
0	0	1	4	6	NM_140170.1	CG14145-RA (CG14145), mRNA
0	0	1	4	5	NM_079924.2	CG18780-RA (MED20), mRNA
0	0	1	4	2	NM_134682.2	CG4133-RA (CG4133), mRNA
0	0	1	3	8	NM_176723.2	CG33175-RG, transcript variant G (spri), mRNA
0	0	1	3	4	NM_135801.2	CG9302-RA (CG9302), mRNA
0	0	1	3	3	NM_141887.1	CG3532-RA (CG3532), mRNA
0	0	1	3	2	NM_079303.2	CG7303-RA (Gr68a), mRNA
0	0	1	2	6	NM_139810.2	CG9953-RA (CG9953), mRNA
0	0	1	2	2	NM_079056.2	CG5784-RB, transcript variant B (Mapmodulin), mRNA
0	0	1	2	2	NM_166258.1	CG5784-RA, transcript variant A (Mapmodulin), mRNA
0	0	0	9	4	NM_080330.2	CG3620-RA, transcript variant A (norpA), mRNA
0	0	0	9	4	NM_001014721.1	CG3620-RC, transcript variant C (norpA), mRNA
0	0	0	9	4	NM_167008.1	CG3620-RB, transcript variant B (norpA), mRNA
0	0	0	9	4	NM_001014720.1	CG3620-RD, transcript variant D (norpA), mRNA
0	0	0	5	6	NM_169906.1	CG4217-RB, transcript variant B (TFAM), mRNA
0	0	0	5	6	NM_079691.2	CG4217-RA, transcript variant A (TFAM), mRNA
0	0	0	5	5	NM_136623.1	CG8014-RA (Rme-8), mRNA
0	0	0	5	3	NM_080516.1	CG16757-RA (Spn), mRNA
0	0	0	5	3	NM_057517.2	CG4824-RA, transcript variant A (BicC), mRNA
0	0	0	5	3	NM_165144.1	CG4824-RB, transcript variant B (BicC), mRNA
0	0	0	5	3	NM_165145.1	CG4824-RD, transcript variant D (BicC), mRNA
0	0	0	4	8	NM_079615.2	CG7756-RA (Hsc70-2), mRNA
0	0	0	4	6	NM_057771.2	CG1864-RB, transcript variant B (Hr38), mRNA
0	0	0	4	6	NM_176065.1	CG1864-RC, transcript variant C (Hr38), mRNA
0	0	0	4	5	NM_142083.2	CG14355-RA (CG14355), mRNA

Back