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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7978R-3

Symbol Ac76E Full Name Adenylyl cyclase 76E

CG No CG7978 Old CG No CG7978

Synonyms CG7978, AC 76E, DAC76E, DAC2, Ac76E

Accession No (Link to NCBI) NM_079449.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGGCAATGGCACAAATGCAAC

Primer Seq. 3'

aatctagaggtaccAGTGGATGCACAGGAAGAGGG

Predicted Fragment Size

IR fragment full Seq

0001 GGGCAATGGC ACAAATGCAA CAGCGAATCT GATTGTTAAG GCCGACGGAA ATGCAACgNA 
0061 GCCGAAGGNG atNACGTCAT CGGCGGCCAG GATGAATGAC GCCCTTTCGG CATCTTTGGC 
0121 CGATTNTGAG CGAGCAGGAA AATGGAACCA CGGCTGAGGA CATCCACCTA NACGATTTGT 
0181 ATACCCGCTA CCGCCAGAGG CTCCGCAAGT CGCTTTTCNA GATCGGGATT GTTAACTTCG 
0241 CTGCTGGCAT GTGTTGTGTC CATAATAATT GGCATCGTAT TACGGTCAGN ACCTGGTGCA 
0301 GACCATGCTC TTAGTCCTGG CTGCCCTAAT CTCGGGATCC ATTCTCACGG CCCTGCAGTT 
0361 cCCGGCGGTG CTGAGCTNCC NGGCAGCCGC CNNGGcNTTC GCCATCGTCA CCACCTTCTC 
0421 ACTGGGCACC ATTGCGGCCA TCACCGGGGA CGAACTGGCT CCCCTGCCCA TGTACGCCCT 
0481 CTTcCTGTGC ATCCACT

in silico PCR Fragment

0001 GGGCAATGGC ACAAATGCAA CAGCGAATCT GATTGTTAAG GCCGACGGAA ATGCAACGCA 
0061 GCCGAAGGCG ATGACGTCAT CGGCGGCCAG GATGAATGAC GCCCTTTCGG CATCTTTGGC 
0121 CGATT-TGAG CGAGCAGGAA AATGGAACCA CGGCTGAGGA CATCCACCTA AACGATTTGT 
0181 ATACCCGCTA CCGCCAGAGG CTCCGCAAGT CGCTTTTC-A GATCGGGATT GTTAACTTCG 
0241 CTGCTGGCAT GTGTTGTGTC CATAATAATT GGCATCGT-T TACGGCCAGC ACCTGGTGCA 
0301 GACCATGCTC TTAGTCCTGG CTGCCCTAAT CTCGGGATCC ATTCTCACGG CCCTGCAGTT 
0361 CCCGGCGGTG CTGAGCTCCC CGGCAGCCGC CTTGGCCTTC GCCATCGTCA CCACCTTCTC 
0421 ACTGGGCACC ATTGCGGCCA TCACCGGGGA CGAACTGGCT CCCCTGCCCA TGTACGCCCT 
0481 CTTCCTGTGC ATCCACT

Assemble Data


7978R-3.IR_full       1   GGGCAATGGCACAAATGCAACAGCGAATCTGATTGTTAAGGCCGACGGAAATGCAACGNA 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
7978R-3.in silico     1   GGGCAATGGCACAAATGCAACAGCGAATCTGATTGTTAAGGCCGACGGAAATGCAACGCA 60


7978R-3.IR_full       61  GCCGAAGGNGATNACGTCATCGGCGGCCAGGATGAATGACGCCCTTTCGGCATCTTTGGC 120
                          |||||||| ||| |||||||||||||||||||||||||||||||||||||||||||||||
7978R-3.in silico     61  GCCGAAGGCGATGACGTCATCGGCGGCCAGGATGAATGACGCCCTTTCGGCATCTTTGGC 120


7978R-3.IR_full       121 CGATTNTGAGCGAGCAGGAAAATGGAACCACGGCTGAGGACATCCACCTANACGATTTGT 180
                          ||||| |||||||||||||||||||||||||||||||||||||||||||| |||||||||
7978R-3.in silico     121 CGATT-TGAGCGAGCAGGAAAATGGAACCACGGCTGAGGACATCCACCTAAACGATTTGT 180


7978R-3.IR_full       181 ATACCCGCTACCGCCAGAGGCTCCGCAAGTCGCTTTTCNAGATCGGGATTGTTAACTTCG 240
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
7978R-3.in silico     181 ATACCCGCTACCGCCAGAGGCTCCGCAAGTCGCTTTTC-AGATCGGGATTGTTAACTTCG 240


7978R-3.IR_full       241 CTGCTGGCATGTGTTGTGTCCATAATAATTGGCATCGTATTACGGTCAGNACCTGGTGCA 300
                          |||||||||||||||||||||||||||||||||||||| |||||| ||| ||||||||||
7978R-3.in silico     241 CTGCTGGCATGTGTTGTGTCCATAATAATTGGCATCGT-TTACGGCCAGCACCTGGTGCA 300


7978R-3.IR_full       301 GACCATGCTCTTAGTCCTGGCTGCCCTAATCTCGGGATCCATTCTCACGGCCCTGCAGTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7978R-3.in silico     301 GACCATGCTCTTAGTCCTGGCTGCCCTAATCTCGGGATCCATTCTCACGGCCCTGCAGTT 360


7978R-3.IR_full       361 CCCGGCGGTGCTGAGCTNCCNGGCAGCCGCCNNGGCNTTCGCCATCGTCACCACCTTCTC 420
                          ||||||||||||||||| || ||||||||||  ||| |||||||||||||||||||||||
7978R-3.in silico     361 CCCGGCGGTGCTGAGCTCCCCGGCAGCCGCCTTGGCCTTCGCCATCGTCACCACCTTCTC 420


7978R-3.IR_full       421 ACTGGGCACCATTGCGGCCATCACCGGGGACGAACTGGCTCCCCTGCCCATGTACGCCCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7978R-3.in silico     421 ACTGGGCACCATTGCGGCCATCACCGGGGACGAACTGGCTCCCCTGCCCATGTACGCCCT 480


7978R-3.IR_full       481 CTTCCTGTGCATCCACT 497
                          |||||||||||||||||
7978R-3.in silico     481 CTTCCTGTGCATCCACT 497

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	476	0	0	4	NM_079449.2	CG7978-RA (Ac76E), mRNA
28.15	134	5	1	5	NM_168827.1	CG32219-RA (CG32219), mRNA
0	0	5	4	2	NM_137628.1	CG13868-RA (CG13868), mRNA
0	0	2	9	10	NM_135440.1	CG4450-RA (CG4450), mRNA
0	0	2	4	7	NM_139680.1	CG17150-RA, transcript variant A (CG17150), mRNA
0	0	2	4	3	NM_141337.1	CG2031-RA (Hpr1), mRNA
0	0	2	3	2	NM_136233.2	CG9273-RA (CG9273), mRNA
0	0	2	2	6	NM_169386.1	CG17228-RA, transcript variant A (pros), mRNA
0	0	2	2	6	NM_176459.1	CG17228-RD, transcript variant D (pros), mRNA
0	0	2	2	6	NM_079593.3	CG17228-RC, transcript variant C (pros), mRNA
0	0	1	5	4	NM_135508.2	CG5739-RA (CG5739), mRNA
0	0	1	4	5	NM_130478.2	CG2995-RA (CG2995), mRNA
0	0	1	3	7	NM_135248.1	CG13776-RA (CG13776), mRNA
0	0	1	3	3	NM_140438.2	CG32139-RA (Sox21b), mRNA
0	0	1	3	2	NM_168107.1	CG17150-RC, transcript variant C (CG17150), mRNA
0	0	1	2	4	NM_176339.1	CG9674-RD, transcript variant D (CG9674), mRNA
0	0	1	2	4	NM_140665.1	CG9674-RA, transcript variant A (CG9674), mRNA
0	0	1	2	4	NM_168156.1	CG7018-RA, transcript variant A (Ets65A), mRNA
0	0	1	2	4	NM_168686.1	CG9674-RB, transcript variant B (CG9674), mRNA
0	0	1	2	4	NM_168687.1	CG9674-RC, transcript variant C (CG9674), mRNA
0	0	1	2	3	NM_142158.1	CG6966-RA (CG6966), mRNA
0	0	1	2	2	NM_132993.2	CG8465-RA, transcript variant A (l(1)G0222), mRNA
0	0	1	2	2	NM_167569.1	CG8465-RB, transcript variant B (l(1)G0222), mRNA
0	0	1	2	2	NM_167570.1	CG8465-RC, transcript variant C (l(1)G0222), mRNA
0	0	1	2	2	NM_135171.2	CG9498-RA (CG9498), mRNA
0	0	0	8	6	NM_167399.4	CG12047-RB, transcript variant B (mud), mRNA
0	0	0	7	17	NM_153771.2	CG8920-RC, transcript variant C (CG8920), mRNA
0	0	0	7	14	NM_137630.2	CG8920-RA, transcript variant A (CG8920), mRNA
0	0	0	7	14	NM_137631.5	CG8920-RB, transcript variant B (CG8920), mRNA
0	0	0	7	2	NM_166067.1	CG10151-RA, transcript variant A (CG10151), mRNA

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