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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7788R-2 |
| Symbol |
Ice |
Full Name |
Ice |
| CG No |
CG7788 |
Old CG No |
CG7788 |
| Synonyms |
drICE, Drice, drice, DrICE, DRICE, ICE, CG7788, DrIce, ice, Ice, caspase 3 |
| Accession No (Link to NCBI) |
NM_079827.2 |
| Inserted Chr. |
X |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Li M, Sun S, Priest J, Bi X, Fan Y.
Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
Cell Death Dis (2019) 10(8) 613
[
PubMed ID = 31409797
]
[
RRC reference
]
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.
Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.
Dev Biol (2017) 427(1) 61-71
[
PubMed ID = 28514643
]
[
RRC reference
]
Zhang S, Chen C, Wu C, Yang Y, Li W, Xue L.
The canonical Wg signaling modulates Bsk-mediated cell death in Drosophila.
Cell Death Dis (2015) 6(4) e1713
[
PubMed ID = 25855961
]
[
RRC reference
]
Khammari A, Agnès F, Gandille P, Pret AM.
Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1.
Cell Death Differ (2011) 18(5) 793-805
[
PubMed ID = 21113144
]
[
RRC reference
]
Leulier F, Ribeiro PS, Palmer E, Tenev T, Takahashi K, Robertson D, Zachariou A, Pichaud F, Ueda R, Meier P.
Systematic in vivo RNAi analysis of putative components of the Drosophila cell death machinery.
Cell Death Differ (2006) 13(10) 1663-74
[
PubMed ID = 16485033
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATCCTCGCATCCTTACGGCA |
| Primer Seq. 3' |
aatctagaggtaccCTGGCAGGCCTGTATGAAGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NATCCTCGCA TCCTTACGGC AGCGGAGccA TTGGGCAGCT GGcCAACGGG TACAGCTCAC
0061 CCTCGTCCAG CTACCGNAAG AATGTAGCCA AAATGGTCAC CGACCGCCAT GCAGCCGAGT
0121 ACAACATGCG CCACAAGAAC CGCGGAATGG CACTGATCTT CAACCATGAG CACTTCGAGG
0181 TGCCCACCTT GAAGTCTCGC GCGGNAACCA ATGTGGACTG CGAGAATCTG ACTCGGGTGC
0241 TCAAGCAGCT GGACTTCGAA GTGACCGTGT ACAAGGACTG CCGCTACAAG GACATTTTGA
0301 GGACAATCGA GTATGCAGCG TCGCAGAATC ACAGCGATAG TGATTGTATC CTGGTCGCCA
0361 TCCTGTCGCA CGGCGAGATG GGCTACATCT ACGCCAAGGA CACACAGTAC AAGCTGGATA
0421 ACATCTGGAG CTTCTTCACG GCCAATCACT GCCCCTCGCT AGCCGGCAAA CCCAAGTTGT
0481 TCTTCATACA GGCCTGCCAG |
| in silico PCR Fragment |
0001 GATCCTCGCA TCCTTACGGC AGCGGAGCCA TTGGGCAGCT GGCCAACGGG TACAGCTCAC
0061 CCTCGTCCAG CTACCGTAAG AATGTAGCCA AAATGGTCAC CGACCGCCAT GCAGCCGAGT
0121 ACAACATGCG CCACAAGAAC CGCGGAATGG CACTGATCTT CAACCATGAG CACTTCGAGG
0181 TGCCCACCTT GAAGTCTCGC GCGGGAACCA ATGTGGACTG CGAGAATCTG ACTCGGGTGC
0241 TCAAGCAGCT GGACTTCGAA GTGACCGTGT ACAAGGACTG CCGCTACAAG GACATTTTGA
0301 GGACAATCGA GTATGCAGCG TCGCAGAATC ACAGCGATAG TGATTGCATC CTGGTCGCCA
0361 TCCTGTCGCA CGGCGAGATG GGCTACATCT ACGCCAAGGA CACACAGTAC AAGCTGGATA
0421 ACATCTGGAG CTTCTTCACG GCCAATCACT GCCCCTCGCT AGCCGGCAAA CCCAAGTTGT
0481 TCTTCATACA GGCCTGCCAG |
| Assemble Data |
7788R-2.IR_full 1 ATCCTCGCATCCTTACGGCAGCGGAGCCATTGGGCAGCTGGCCAACGGGTACAGCTCAC 59
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7788R-2.in silico 1 ATCCTCGCATCCTTACGGCAGCGGAGCCATTGGGCAGCTGGCCAACGGGTACAGCTCAC 59
7788R-2.IR_full 61 CCTCGTCCAGCTACCGNAAGAATGTAGCCAAAATGGTCACCGACCGCCATGCAGCCGAGT 119
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7788R-2.in silico 61 CCTCGTCCAGCTACCGTAAGAATGTAGCCAAAATGGTCACCGACCGCCATGCAGCCGAGT 119
7788R-2.IR_full 121 ACAACATGCGCCACAAGAACCGCGGAATGGCACTGATCTTCAACCATGAGCACTTCGAGG 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7788R-2.in silico 121 ACAACATGCGCCACAAGAACCGCGGAATGGCACTGATCTTCAACCATGAGCACTTCGAGG 179
7788R-2.IR_full 181 TGCCCACCTTGAAGTCTCGCGCGGNAACCAATGTGGACTGCGAGAATCTGACTCGGGTGC 239
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7788R-2.in silico 181 TGCCCACCTTGAAGTCTCGCGCGGGAACCAATGTGGACTGCGAGAATCTGACTCGGGTGC 239
7788R-2.IR_full 241 TCAAGCAGCTGGACTTCGAAGTGACCGTGTACAAGGACTGCCGCTACAAGGACATTTTGA 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7788R-2.in silico 241 TCAAGCAGCTGGACTTCGAAGTGACCGTGTACAAGGACTGCCGCTACAAGGACATTTTGA 299
7788R-2.IR_full 301 GGACAATCGAGTATGCAGCGTCGCAGAATCACAGCGATAGTGATTGTATCCTGGTCGCCA 359
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
7788R-2.in silico 301 GGACAATCGAGTATGCAGCGTCGCAGAATCACAGCGATAGTGATTGCATCCTGGTCGCCA 359
7788R-2.IR_full 361 TCCTGTCGCACGGCGAGATGGGCTACATCTACGCCAAGGACACACAGTACAAGCTGGATA 419
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7788R-2.in silico 361 TCCTGTCGCACGGCGAGATGGGCTACATCTACGCCAAGGACACACAGTACAAGCTGGATA 419
7788R-2.IR_full 421 ACATCTGGAGCTTCTTCACGGCCAATCACTGCCCCTCGCTAGCCGGCAAACCCAAGTTGT 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7788R-2.in silico 421 ACATCTGGAGCTTCTTCACGGCCAATCACTGCCCCTCGCTAGCCGGCAAACCCAAGTTGT 479
7788R-2.IR_full 481 TCTTCATACAGGCCTGCCAG 499
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7788R-2.in silico 481 TCTTCATACAGGCCTGCCAG 499
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_079827.2 |
CG7788-RA (Ice), mRNA |
| 0.2 |
1 |
3 |
3 |
7 |
NM_001043217.1 |
CG34113-RP, transcript variant P (CG34113), mRNA |
| 0.2 |
1 |
3 |
3 |
6 |
NM_001043216.1 |
CG34113-RO, transcript variant O (CG34113), mRNA |
| 0 |
0 |
6 |
31 |
66 |
NM_057626.3 |
CG5370-RA (Dcp-1), mRNA |
| 0 |
0 |
6 |
4 |
4 |
NM_142487.1 |
CG7709-RA (CG7709), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_142112.2 |
CG8279-RA (Pde6), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_165461.1 |
CG1765-RA, transcript variant A (EcR), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134880.1 |
CG3104-RA, transcript variant A (CG3104), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164505.1 |
CG3104-RB, transcript variant B (CG3104), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164749.1 |
CG5261-RA, transcript variant A (CG5261), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_135274.2 |
CG5261-RB, transcript variant B (CG5261), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134864.3 |
CG2848-RA (Trn-SR), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135922.2 |
CG3793-RA (CG3793), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_136647.2 |
CG8809-RA (Camta), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079458.2 |
CG5517-RA (Ide), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001043240.1 |
CG34114-RB (CG34114), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_139808.1 |
CG10064-RA (CG10064), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_165325.1 |
CG10076-RC, transcript variant C (spir), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080115.2 |
CG10076-RB, transcript variant B (spir), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_165323.1 |
CG10076-RA, transcript variant A (spir), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135892.2 |
CG4185-RA (NC2beta), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_139791.1 |
CG10144-RA (CG10144), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132871.2 |
CG8939-RA (CG8939), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_143627.1 |
CG11563-RA (CG11563), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_169698.1 |
CG5000-RA (msps), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_057243.3 |
CG3228-RA (kz), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_057598.3 |
CG10739-RA (pigeon), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_001038906.1 |
CG17352-RD, transcript variant D (CG17352), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_139957.2 |
CG17352-RC, transcript variant C (CG17352), mRNA |
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