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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7626R-3 |
| Symbol |
Spt5 |
Full Name |
Spt5 |
| CG No |
CG7626 |
Old CG No |
CG7626 |
| Synonyms |
DSIF, spt5, dDSIF, dSpt5, Dspt5, CG7626, Spt5 |
| Accession No (Link to NCBI) |
NM_144353.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Szlanka T, Lukacsovich T, Bálint É, Virágh E, Szabó K, Hajdu I, Molnár E, Lin YH, Zvara Á, Kelemen-Valkony I, Méhi O, Török I, Hegedűs Z, Kiss B, Ramasz B, Magdalena LM, Puskás L, Mechler BM, Fónagy A, Asztalos Z, Steinbach G, Žurovec M, Boros I, Kiss I.<br> Dominant suppressor genes of p53-induced apoptosis in Drosophila melanogaster.<br> G3 (Bethesda) (2024) 14(9)
[
PubMed ID = 38985658
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTAATCGCGGCAAGAGCGATGA |
| Primer Seq. 3' |
aatctagaggtaccGCATTAGAAGAAGCGCTGTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TAATCGCGGC AAGAGCGATG AATCCGGCGA AGAGGAGGAG GAGCCACCaG GTNAGGACAT
0061 CGATTCCGAG GAGTACGATG AGGAGGAAAA CGACGATCAC CCAAGGAAGA AGAAAAAGAA
0121 GGAGCGTTTT GGGGGCTTCA TCATTGACGA GGCCGAAGTG GACGATGAAG TCGATGAAGA
0181 TGACGAGTGG GAAGAGGGTG CCAATGAAAT TGGCATTGTT GGCAATGAGA TCGATGAGCT
0241 GGGCCCCACA GCCAGGGATA TTGAAATTCG TCGACGAGGC ACAAACCTTT GGGACACTCA
0301 AAAGGAGGAC GAGATAGAGG AGTACCTCCG TAAGAAGTAC GCCGATGAGT CAATTGCAAA
0361 ACGCCACTTT GGAGATGGCG GTGAAGAGAT GTCAGACGAA ATTACCCAAC AAACTCTATT
0421 GCCCGGCATC AAGGATCCCN AATTTGTGGG ATGGTNGAAA TGTCGTATAG GCGAGGAGAA
0481 GGCCACAGCG CTTCTTCTaA TGC |
| in silico PCR Fragment |
0001 TAATCGCGGC AAGAGCGATG AATCCGGCGA AGAGGAGGAG GAGCCACCAG GTGAGGACAT
0061 CGATTCCGAG GAGTACGATG AGGAGGAGAA CGACGATCAC CCAAGGAAGA AGAAAAAGAA
0121 GGAGCGTTTT GGGGGCTTCA TCATTGACGA GGCCGAAGTG GACGATGAAG TCGATGAAGA
0181 TGACGAGTGG GAAGAGGGTG CCAATGAAAT TGGCATTGTT GGCAATGAGA TCGATGAGCT
0241 GGGCCCCACA GCCAGGGATA TTGAAATTCG ACGACGAGGC ACAAACCTTT GGGACACTCA
0301 AAAGGAGGAC GAGATAGAGG AGTATCTCCG TAAGAAGTAC GCCGATGAGT CAATTGCAAA
0361 ACGCCACTTT GGAGATGGCG GTGAAGAGAT GTCAGACGAA ATTACCCAAC AAACTCTATT
0421 GCCCGGCATC AAGGATCCC- AATTTGT-GG ATGGT-GAAA TGTCGTATAG GCGAGGAGAA
0481 GGCCACAGCG CTTCTTCTAA TGC |
| Assemble Data |
<PRE>
7626R-3.IR_full 1 TAATCGCGGCAAGAGCGATGAATCCGGCGAAGAGGAGGAGGAGCCACCAGGT<span class="snp"><tt>N</tt></span>AGGACAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||
7626R-3.in silico 1 TAATCGCGGCAAGAGCGATGAATCCGGCGAAGAGGAGGAGGAGCCACCAGGT<span class="snp"><tt>G</tt></span>AGGACAT 60
7626R-3.IR_full 61 CGATTCCGAGGAGTACGATGAGGAGGA<span class="snp"><tt>A</tt></span>AACGACGATCACCCAAGGAAGAAGAAAAAGAA 120
|||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||
7626R-3.in silico 61 CGATTCCGAGGAGTACGATGAGGAGGA<span class="snp"><tt>G</tt></span>AACGACGATCACCCAAGGAAGAAGAAAAAGAA 120
7626R-3.IR_full 121 GGAGCGTTTTGGGGGCTTCATCATTGACGAGGCCGAAGTGGACGATGAAGTCGATGAAGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7626R-3.in silico 121 GGAGCGTTTTGGGGGCTTCATCATTGACGAGGCCGAAGTGGACGATGAAGTCGATGAAGA 180
7626R-3.IR_full 181 TGACGAGTGGGAAGAGGGTGCCAATGAAATTGGCATTGTTGGCAATGAGATCGATGAGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7626R-3.in silico 181 TGACGAGTGGGAAGAGGGTGCCAATGAAATTGGCATTGTTGGCAATGAGATCGATGAGCT 240
7626R-3.IR_full 241 GGGCCCCACAGCCAGGGATATTGAAATTCG<span class="snp"><tt>T</tt></span>CGACGAGGCACAAACCTTTGGGACACTCA 300
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||
7626R-3.in silico 241 GGGCCCCACAGCCAGGGATATTGAAATTCG<span class="snp"><tt>A</tt></span>CGACGAGGCACAAACCTTTGGGACACTCA 300
7626R-3.IR_full 301 AAAGGAGGACGAGATAGAGGAGTA<span class="snp"><tt>C</tt></span>CTCCGTAAGAAGTACGCCGATGAGTCAATTGCAAA 360
||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||
7626R-3.in silico 301 AAAGGAGGACGAGATAGAGGAGTA<span class="snp"><tt>T</tt></span>CTCCGTAAGAAGTACGCCGATGAGTCAATTGCAAA 360
7626R-3.IR_full 361 ACGCCACTTTGGAGATGGCGGTGAAGAGATGTCAGACGAAATTACCCAACAAACTCTATT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7626R-3.in silico 361 ACGCCACTTTGGAGATGGCGGTGAAGAGATGTCAGACGAAATTACCCAACAAACTCTATT 420
7626R-3.IR_full 421 GCCCGGCATCAAGGATCCC<span class="snp"><tt>N</tt></span>AATTTGT<span class="snp"><tt>G</tt></span>GGATGGT<span class="snp"><tt>N</tt></span>GAAATGTCGTATAGGCGAGGAGAA 480
|||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
7626R-3.in silico 421 GCCCGGCATCAAGGATCCC<span class="snp"><tt>-</tt></span>AATTTGT<span class="snp"><tt>-</tt></span>GGATGGT<span class="snp"><tt>-</tt></span>GAAATGTCGTATAGGCGAGGAGAA 480
7626R-3.IR_full 481 GGCCACAGCGCTTCTTCTAATGC 503
|||||||||||||||||||||||
7626R-3.in silico 481 GGCCACAGCGCTTCTTCTAATGC 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_144353.2 |
CG7626-RA, transcript variant A (Spt5), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_206167.1 |
CG7626-RB, transcript variant B (Spt5), mRNA |
| 0.41 |
2 |
4 |
2 |
2 |
NM_135827.2 |
CG16890-RA, transcript variant A (CG16890), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_143015.2 |
CG13625-RA (CG13625), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_001032107.1 |
CG7100-RK, transcript variant K (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_001032109.1 |
CG7100-RI, transcript variant I (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_001032108.1 |
CG7100-RJ, transcript variant J (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165231.1 |
CG7100-RE, transcript variant E (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165230.1 |
CG7100-RG, transcript variant G (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165229.1 |
CG7100-RA, transcript variant A (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165228.1 |
CG7100-RC, transcript variant C (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165227.1 |
CG7100-RD, transcript variant D (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165226.1 |
CG7100-RF, transcript variant F (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165225.1 |
CG7100-RB, transcript variant B (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_165224.1 |
CG7100-RH, transcript variant H (CadN), mRNA |
| 0.2 |
1 |
2 |
4 |
8 |
NM_001032106.1 |
CG7100-RL, transcript variant L (CadN), mRNA |
| 0.2 |
1 |
2 |
2 |
4 |
NM_134888.2 |
CG3165-RA (CG3165), mRNA |
| 0 |
0 |
5 |
11 |
13 |
NM_137047.2 |
CG6197-RA (CG6197), mRNA |
| 0 |
0 |
5 |
9 |
22 |
NM_079979.2 |
CG18497-RB, transcript variant B (spen), mRNA |
| 0 |
0 |
5 |
9 |
22 |
NM_164374.1 |
CG18497-RA, transcript variant A (spen), mRNA |
| 0 |
0 |
5 |
9 |
22 |
NM_164375.1 |
CG18497-RC, transcript variant C (spen), mRNA |
| 0 |
0 |
5 |
2 |
8 |
NM_140060.1 |
CG3445-RA (phol), mRNA |
| 0 |
0 |
4 |
4 |
10 |
NM_058131.3 |
CG6712-RA (CG6712), mRNA |
| 0 |
0 |
4 |
4 |
5 |
NM_136480.2 |
CG1553-RA, transcript variant A (CG1553), mRNA |
| 0 |
0 |
4 |
4 |
5 |
NM_165558.1 |
CG1553-RC, transcript variant C (CG1553), mRNA |
| 0 |
0 |
4 |
4 |
5 |
NM_165557.1 |
CG1553-RB, transcript variant B (CG1553), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_166968.1 |
CG32498-RN, transcript variant N (dnc), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_166970.1 |
CG32498-RG, transcript variant G (dnc), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_166967.1 |
CG32498-RA, transcript variant A (dnc), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_166966.1 |
CG32498-RM, transcript variant M (dnc), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
dme03250
:
Viral life cycle - HIV-1
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