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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7590R-1

Symbol

scyl

Full Name

scylla

CG No

CG7590

Old CG No

CG7590

Synonyms

scy, CG7590, anon-WO0118547.215, scyl

Accession No (Link to NCBI)

NM_140199.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Hwang SH, Bang S, Kim W, Chung J.<br> Von Hippel-Lindau tumor suppressor (VHL) stimulates TOR signaling by interacting with phosphoinositide 3-kinase (PI3K).<br> J Biol Chem (2020) 295(8) 2336-2347 [ PubMed ID = 31959630 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGGCATACACTCTGGACCTG

Primer Seq. 3'

aatctagaggtaccATCGAGGCAATGCGACGCGAA

Predicted Fragment Size

IR fragment full Seq

0001 TCGGCATACA CTCTGGACCT GATGTCCAAG AAGGCCAAGA CGACAACCGG GGGCAGCAGC 
0061 AATGGCAGCa ATNCTACCGC CACATCCACA ACCACATCCA CATCCAGCAG TATTAAGCAC 
0121 AAGCAGCCGG CGGGCAGCAG CNAACAACAA TGTTGGCCAA AGTCAGAGCA AGAAGACAAA 
0181 GCCCAGCGGC AGCTATAATA GCAATAGCAA CTACTACTAC TACGCTGCTG ACGAGGAGGA 
0241 GGGCGGTAGC GCTGACTATG CACTGAGCAA CTACGATAAG AAGGCTGTCG AGGAGCTGTC 
0301 CCTGCGATTG CTCGACGAAC TGAGGGCGGC CAAGTCGCGT CATTTGACCT GTACGGAAGT 
0361 GTCGCTACCC TGTGATCTcN CGcCGAGCGT GGCCCGGGAA ATAATCCGCG TGTCGGAGAA 
0421 GGAGcCCCGC gGCATACGCG GCTGCACCAT CTACATTGAG TTCGAGGATG AGCCGAAGAA 
0481 TTCgNGTCGC ATTGCCTCGA T

in silico PCR Fragment

0001 TCGGCATACA CTCTGGACCT GATGTCCAAG AAGGCCAAGA CGACAACCGG GGGCAGCAGC 
0061 AATGGCAGCA ATGCTACCGC CACATCCACA ACCACATCCA CATCCAGCAG TATTAAGCAC 
0121 AAGCAGCCGG CGGGCAGCAG C-AACAACAA TGTTGGCCAA AGTCAGAGCA AGAAGACAAA 
0181 GCCCAGCGGC AGCTATAATA GCAATAGCAA CTACTACTAC TACGCTGCTG ACGAGGAGGA 
0241 GGGCGGTAGC GCTGACTATG CACTGAGCAA CTACGATAAG AAGGCTGTCG AGGAGCTGTC 
0301 CCTGCGATTG CTCGACGAAC TGAGGGCGGC CAAGTCGCGT CATTTGACCT GTACGGAAGT 
0361 GTCGCTACCC TGTGATCTCA CGCCGAGCGT GGCCCGGGAA ATAATCCGCG TGTCGGAGAA 
0421 GGAGCCCCGC GGCATACGCG GCTGCACCAT CTACATTGAG TTCGAGGATG AGCCGAAGAA 
0481 TTCGCGTCGC ATTGCCTCGA T

Assemble Data

<PRE>

7590R-1.IR_full       1   TCGGCATACACTCTGGACCTGATGTCCAAGAAGGCCAAGACGACAACCGGGGGCAGCAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     1   TCGGCATACACTCTGGACCTGATGTCCAAGAAGGCCAAGACGACAACCGGGGGCAGCAGC 60


7590R-1.IR_full       61  AATGGCAGCAAT<span class="snp"><tt>N</tt></span>CTACCGCCACATCCACAACCACATCCACATCCAGCAGTATTAAGCAC 120
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     61  AATGGCAGCAAT<span class="snp"><tt>G</tt></span>CTACCGCCACATCCACAACCACATCCACATCCAGCAGTATTAAGCAC 120


7590R-1.IR_full       121 AAGCAGCCGGCGGGCAGCAGC<span class="snp"><tt>N</tt></span>AACAACAATGTTGGCCAAAGTCAGAGCAAGAAGACAAA 180
                          |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     121 AAGCAGCCGGCGGGCAGCAGC<span class="snp"><tt>-</tt></span>AACAACAATGTTGGCCAAAGTCAGAGCAAGAAGACAAA 180


7590R-1.IR_full       181 GCCCAGCGGCAGCTATAATAGCAATAGCAACTACTACTACTACGCTGCTGACGAGGAGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     181 GCCCAGCGGCAGCTATAATAGCAATAGCAACTACTACTACTACGCTGCTGACGAGGAGGA 240


7590R-1.IR_full       241 GGGCGGTAGCGCTGACTATGCACTGAGCAACTACGATAAGAAGGCTGTCGAGGAGCTGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     241 GGGCGGTAGCGCTGACTATGCACTGAGCAACTACGATAAGAAGGCTGTCGAGGAGCTGTC 300


7590R-1.IR_full       301 CCTGCGATTGCTCGACGAACTGAGGGCGGCCAAGTCGCGTCATTTGACCTGTACGGAAGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     301 CCTGCGATTGCTCGACGAACTGAGGGCGGCCAAGTCGCGTCATTTGACCTGTACGGAAGT 360


7590R-1.IR_full       361 GTCGCTACCCTGTGATCTC<span class="snp"><tt>N</tt></span>CGCCGAGCGTGGCCCGGGAAATAATCCGCGTGTCGGAGAA 420
                          |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     361 GTCGCTACCCTGTGATCTC<span class="snp"><tt>A</tt></span>CGCCGAGCGTGGCCCGGGAAATAATCCGCGTGTCGGAGAA 420


7590R-1.IR_full       421 GGAGCCCCGCGGCATACGCGGCTGCACCATCTACATTGAGTTCGAGGATGAGCCGAAGAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7590R-1.in silico     421 GGAGCCCCGCGGCATACGCGGCTGCACCATCTACATTGAGTTCGAGGATGAGCCGAAGAA 480


7590R-1.IR_full       481 TTCG<span class="snp"><tt>N</tt></span>GTCGCATTGCCTCGAT 501
                          ||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
7590R-1.in silico     481 TTCG<span class="snp"><tt>C</tt></span>GTCGCATTGCCTCGAT 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	6	7	NM_206321.1	CG7590-RB, transcript variant B (scyl), mRNA
100	482	0	6	7	NM_140199.2	CG7590-RA, transcript variant A (scyl), mRNA
0.41	2	3	5	4	NM_137895.1	CG3800-RA (CG3800), mRNA
0.41	2	2	2	7	NM_057506.2	CG7672-RA, transcript variant A (gl), mRNA
0.41	2	2	2	7	NM_169807.2	CG7672-RB, transcript variant B (gl), mRNA
0.41	2	2	2	3	NM_137120.1	CG17386-RA (CG17386), mRNA
0.2	1	2	5	10	NM_132858.2	CG9056-RA (CG9056), mRNA
0	0	16	13	19	NM_134530.2	CG9576-RA (CG9576), mRNA
0	0	14	14	12	NM_167663.1	CG3917-RB, transcript variant B (Grip84), mRNA
0	0	14	14	12	NM_078685.2	CG3917-RC, transcript variant C (Grip84), mRNA
0	0	14	14	12	NM_167662.1	CG3917-RA, transcript variant A (Grip84), mRNA
0	0	9	19	33	NM_135079.4	CG14021-RB, transcript variant B (CG14021), mRNA
0	0	9	19	33	NM_164640.1	CG14021-RA, transcript variant A (CG14021), mRNA
0	0	9	6	9	NM_134807.1	CG15383-RA (CG15383), mRNA
0	0	7	15	11	NM_078581.2	CG1500-RA (fw), mRNA
0	0	7	9	15	NM_137707.2	CG30388-RA (Magi), mRNA
0	0	7	6	14	NM_139418.1	CG1887-RA (CG1887), mRNA
0	0	5	10	18	NM_132667.1	CG15756-RA (CG15756), mRNA
0	0	5	6	15	NM_142041.1	CG14368-RA (CG14368), mRNA
0	0	4	35	35	NM_132282.1	CG3898-RA (CG3898), mRNA
0	0	4	9	36	NM_078797.2	CG13109-RA (tai), mRNA
0	0	4	4	11	NM_001014557.1	CG33545-RA (nab), mRNA
0	0	4	2	13	NM_169874.1	CG4608-RA (bnl), mRNA
0	0	3	6	17	NM_001042891.1	CG31761-RE, transcript variant E (bru-2), mRNA
0	0	3	6	10	NM_165007.1	CG31761-RC, transcript variant C (bru-2), mRNA
0	0	3	6	10	NM_176025.1	CG31761-RD, transcript variant D (bru-2), mRNA
0	0	3	6	10	NM_135715.2	CG31761-RA, transcript variant A (bru-2), mRNA
0	0	3	6	9	NM_057361.3	CG4316-RA (Sb), mRNA
0	0	3	4	25	NM_169110.1	CG31554-RA (CG31554), mRNA
0	0	3	4	13	NM_079001.2	CG3886-RA (Psc), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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