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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7525R-2

Symbol

Tie

Full Name

Tie-like receptor tyrosine kinase

CG No

CG7525

Old CG No

CG7525

Synonyms

tie, RTK, CG7525, DPR1, Tie

Accession No (Link to NCBI)

NM_079204.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Ostalé CM, Esteban N, López-Varea A, de Celis JF.<br> Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing.<br> G3 (Bethesda) (2021) 11(12) [ PubMed ID = 34599799 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTGTGTGTCGGTCTGTGCGAA

Primer Seq. 3'

aatctagaggtaccGTGCAATCGAGGGTCGATTGG

Predicted Fragment Size

IR fragment full Seq

0001 GTGTGTGTCG GTCTGTGCGA AAACGAACAA TAGAAAACCA GATAGAAAAT CAGCAGAGAA 
0061 AACAATCAGC CAAAGCAGCA CAGCCAGAGC AGCAACTATA ATTTTAGTAC TTTTGTTTAT 
0121 ATATTTTTTT CTCGCTATTT GGTGTATGCT TCGGTTACGA TGTCGTTGAT GAAAATCCAT 
0181 CGAGGCAATG AATCATTTTG CAGTTGCTGG TTACTGTTGC TGTTGCTGGT GGCATTGTTG 
0241 TTGGCTGAGG CAGATAGTGG CAGCGGCAGA CGGCAACATT GAGCGAAACG CAGACGAATG 
0301 CAACTAATGG TGCAATTAGA GAGCCGCTCT GTGCAACTGC AAACTGCAAC AGCAGCAACT 
0361 TCAACTTCAA TTGGCAGTTG AATGCGACCG CCGGCGACGG CGAAAGCCGA AATTCAGCGG 
0421 AAATTTTCAG cCCCGGCGAT GTGGATGTTG TTGGTGATAA ACCCACTACT TATAGGCCCT 
0481 CGGGTGCCAA TCGACCCTCG ATTGCAC

in silico PCR Fragment

0001 GTGTGTGTCG GTCTGTGCGA AAACGAACAA TAGAAAACCA GATAGAAAAT CAGCAGAGAA 
0061 AACAATCAGC CAAAGCAGCA CAGCCAGAGC AGCAACTATA ATTTTAGTAC TTTTGTT--T 
0121 ATATTTTTTT CTCGCTATTT GGTGTATGCT TCGGTTACGA TGTCGTTGAT GAAAATCCAT 
0181 CGAGGCAATG AATCATTTTG CAGTTGCTGG TTACTGTTGC TGTTGCTGGT GGCATTGTTG 
0241 TTGGCTGAGG C-GATAGTGG CAGCGGCAG- CGGCAACATT GAGCGAAACG CAGACGAATG 
0301 CAACTAATGG TGCAATTAGA GAGCCGC-CT GTGCAACTGC -AACTGCAAC AGCAGCAACT 
0361 TCAACTTCAA TT-GCAGTTG AATGCGACCG CCGGCGACGG CGAAAGCCGA AATTCAGCGG 
0421 AAATTTTCAG CCCCGGCGAT GTGGATGTTG TTGGTGATAA ACCCACTACT TATAGGCCCT 
0481 CGGGTGCCAA TCGACCCTCG ATTGCAC

Assemble Data

<PRE>

7525R-2.IR_full       1   GTGTGTGTCGGTCTGTGCGAAAACGAACAATAGAAAACCAGATAGAAAATCAGCAGAGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7525R-2.in silico     1   GTGTGTGTCGGTCTGTGCGAAAACGAACAATAGAAAACCAGATAGAAAATCAGCAGAGAA 60


7525R-2.IR_full       61  AACAATCAGCCAAAGCAGCACAGCCAGAGCAGCAACTATAATTTTAGTACTTTTGTT<span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span>T 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|
7525R-2.in silico     61  AACAATCAGCCAAAGCAGCACAGCCAGAGCAGCAACTATAATTTTAGTACTTTTGTT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>T 120


7525R-2.IR_full       121 ATATTTTTTTCTCGCTATTTGGTGTATGCTTCGGTTACGATGTCGTTGATGAAAATCCAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7525R-2.in silico     121 ATATTTTTTTCTCGCTATTTGGTGTATGCTTCGGTTACGATGTCGTTGATGAAAATCCAT 180


7525R-2.IR_full       181 CGAGGCAATGAATCATTTTGCAGTTGCTGGTTACTGTTGCTGTTGCTGGTGGCATTGTTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7525R-2.in silico     181 CGAGGCAATGAATCATTTTGCAGTTGCTGGTTACTGTTGCTGTTGCTGGTGGCATTGTTG 240


7525R-2.IR_full       241 TTGGCTGAGGC<span class="snp"><tt>A</tt></span>GATAGTGGCAGCGGCAG<span class="snp"><tt>A</tt></span>CGGCAACATTGAGCGAAACGCAGACGAATG 300
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
7525R-2.in silico     241 TTGGCTGAGGC<span class="snp"><tt>-</tt></span>GATAGTGGCAGCGGCAG<span class="snp"><tt>-</tt></span>CGGCAACATTGAGCGAAACGCAGACGAATG 300


7525R-2.IR_full       301 CAACTAATGGTGCAATTAGAGAGCCGC<span class="snp"><tt>T</tt></span>CTGTGCAACTGC<span class="snp"><tt>A</tt></span>AACTGCAACAGCAGCAACT 360
                          |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
7525R-2.in silico     301 CAACTAATGGTGCAATTAGAGAGCCGC<span class="snp"><tt>-</tt></span>CTGTGCAACTGC<span class="snp"><tt>-</tt></span>AACTGCAACAGCAGCAACT 360


7525R-2.IR_full       361 TCAACTTCAATT<span class="snp"><tt>G</tt></span>GCAGTTGAATGCGACCGCCGGCGACGGCGAAAGCCGAAATTCAGCGG 420
                          ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
7525R-2.in silico     361 TCAACTTCAATT<span class="snp"><tt>-</tt></span>GCAGTTGAATGCGACCGCCGGCGACGGCGAAAGCCGAAATTCAGCGG 420


7525R-2.IR_full       421 AAATTTTCAGCCCCGGCGATGTGGATGTTGTTGGTGATAAACCCACTACTTATAGGCCCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7525R-2.in silico     421 AAATTTTCAGCCCCGGCGATGTGGATGTTGTTGGTGATAAACCCACTACTTATAGGCCCT 480


7525R-2.IR_full       481 CGGGTGCCAATCGACCCTCGATTGCAC 507
                          |||||||||||||||||||||||||||
7525R-2.in silico     481 CGGGTGCCAATCGACCCTCGATTGCAC 507

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	6	9	NM_079204.1	CG7525-RA (Tie), mRNA
1.24	6	7	46	173	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
1.24	6	7	46	173	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
1.24	6	7	46	173	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
1.24	6	7	46	173	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
1.03	5	3	31	95	NM_137081.2	CG30483-RA (Prosap), mRNA
0.82	4	9	15	32	NM_138213.2	CG13897-RA (CG13897), mRNA
0.62	3	6	10	43	NM_166453.1	CG30282-RA (CG30282), mRNA
0.62	3	5	5	54	NM_132122.2	CG3126-RA, transcript variant A (C3G), mRNA
0.62	3	5	5	51	NM_176694.1	CG3126-RB, transcript variant B (C3G), mRNA
0.41	2	7	3	14	NM_143392.2	CG14521-RA (CG14521), mRNA
0.41	2	4	2	22	NM_141518.1	CG7549-RA, transcript variant A (CG7549), mRNA
0.41	2	4	2	22	NM_001043221.1	CG7549-RC, transcript variant C (CG7549), mRNA
0.2	1	12	98	214	NM_132288.1	CG15365-RA (CG15365), mRNA
0.2	1	9	8	31	NM_001043271.1	CG17299-RL, transcript variant L (SNF4Agamma), mRNA
0.2	1	9	8	31	NM_169950.1	CG17299-RG, transcript variant G (SNF4Agamma), mRNA
0.2	1	8	18	47	NM_164869.1	CG18660-RA, transcript variant A (Nckx30C), mRNA
0.2	1	8	18	47	NM_164870.1	CG18660-RB, transcript variant B (Nckx30C), mRNA
0.2	1	8	18	47	NM_143754.2	CG18660-RC, transcript variant C (Nckx30C), mRNA
0.2	1	8	15	24	NM_057714.3	CG12296-RA (klu), mRNA
0.2	1	5	10	89	NM_079409.2	CG8127-RA, transcript variant A (Eip75B), mRNA
0.2	1	5	9	59	NM_132328.1	CG17446-RA (CG17446), mRNA
0.2	1	4	11	18	NM_137150.2	CG10209-RA (CG10209), mRNA
0.2	1	4	8	56	NM_001014630.1	CG33547-RA (Rim), mRNA
0.2	1	3	9	27	NM_137093.4	CG30069-RA (CG30069), mRNA
0.2	1	3	7	23	NM_169548.1	CG9351-RB, transcript variant B (flfl), mRNA
0.2	1	3	7	23	NM_142047.1	CG9351-RA, transcript variant A (flfl), mRNA
0.2	1	3	7	23	NM_169549.1	CG9351-RC, transcript variant C (flfl), mRNA
0.2	1	3	5	7	NM_170383.1	CG31047-RA (CG31047), mRNA
0.2	1	3	3	10	NM_168108.1	CG32233-RA (CG32233), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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