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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7470R-3

Symbol

CG7470

Full Name

CG7470

CG No

CG7470

Old CG No

CG7470

Synonyms

CG7470

Accession No (Link to NCBI)

NM_141118.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311 [ PubMed ID = 34551427 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGGCCCAGAGCCTTCGAAAC

Primer Seq. 3'

aatctagaggtaccGTCACGAGCACCTGGGCTATC

Predicted Fragment Size

IR fragment full Seq

0001 CTGGCCCAGA GCCTTCGAAA CGGCTTCTAC AGAAACGCCT GGCGAGCGTT CAGcTCCCAC 
0061 GGACCCCGGC AGCCACTGGT TTCGCCGGAG CGNCgCNTGG AAAAGGCGCA TCCAACCTTC 
0121 ACGGAAAGGA GTCAGTTGAA GTACGCCCGG AgGNTGGTGG TCAAGTTGGG CAGTGCAGTC 
0181 ATTACTCGAG AGGACAACCA TGGACTAGCC CTCgGNCGTC TgGCCTCNTA TTGTGGAGCA 
0241 GGTTGCCGAG TGCCATTTGG AAGGACGTGA AGTGATGATG GTCACCAGCG GAGCGGTGGC 
0301 CTNTTGGAAA GCAGAAGCTC GCCCAGGAGC TGCTCATGTC GCTGTCCATG CGCGAGACAC 
0361 TAAACCCAAA AGATAGCAAA GAGTTTGACG GCGCTACGCT GGAACCGCGA GCAGCGGCTG 
0421 CGGTGGNGTC AGTCGGGTCT GATGTCCCTC TACGACGCGA TGTTTGCCCA GTACGGCGTC 
0481 AAGATAGCCC AGGTGCTCGT GAC

in silico PCR Fragment

0001 CTGGCCCAGA GCCTTCGAAA CGGCTTCTAC AGAAACGCCT GGCGAGCGTT CAGCTCCCAC 
0061 GGACCCCGGC AGCCACTGGT TTCGCCGGAG CGGCGCTTGG AAAAGGCGCA TCCAACCTTC 
0121 ACGGAAAGGA GTCAGTTGAA GTACGCCCGG AGGCTGGTGG TCAAGTTGGG CAGTGCAGTC 
0181 ATTACTCGAG AGGACAACCA TGGACTAGCC CTCGGCCGTC TGGCCTC-TA TTGTGGAGCA 
0241 GGTTGCCGAG TGCCATTTGG AAGGACGTGA AGTGATGATG GTCACCAGCG GAGCGGTGGC 
0301 CT-TTGGAAA GCAGAAGCTC GCCCAGGAGC TGCTCATGTC GCTGTCCATG CGCGAGACAC 
0361 TAAACCCAAA AGATAGCAAA GAGTTTGACG GCGCTACGCT GGAACCGCGA GCAGCGGCTG 
0421 CGGTGG-GTC AGTCGGGTCT GATGTCCCTC TACGACGCGA TGTTTGCCCA GTACGGCGTC 
0481 AAGATAGCCC AGGTGCTCGT GAC

Assemble Data

<PRE>

7470R-3.IR_full       1   CTGGCCCAGAGCCTTCGAAACGGCTTCTACAGAAACGCCTGGCGAGCGTTCAGCTCCCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7470R-3.in silico     1   CTGGCCCAGAGCCTTCGAAACGGCTTCTACAGAAACGCCTGGCGAGCGTTCAGCTCCCAC 60


7470R-3.IR_full       61  GGACCCCGGCAGCCACTGGTTTCGCCGGAGCG<span class="snp"><tt>N</tt></span>CGC<span class="snp"><tt>N</tt></span>TGGAAAAGGCGCATCCAACCTTC 120
                          ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
7470R-3.in silico     61  GGACCCCGGCAGCCACTGGTTTCGCCGGAGCG<span class="snp"><tt>G</tt></span>CGC<span class="snp"><tt>T</tt></span>TGGAAAAGGCGCATCCAACCTTC 120


7470R-3.IR_full       121 ACGGAAAGGAGTCAGTTGAAGTACGCCCGGAGG<span class="snp"><tt>N</tt></span>TGGTGGTCAAGTTGGGCAGTGCAGTC 180
                          |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
7470R-3.in silico     121 ACGGAAAGGAGTCAGTTGAAGTACGCCCGGAGG<span class="snp"><tt>C</tt></span>TGGTGGTCAAGTTGGGCAGTGCAGTC 180


7470R-3.IR_full       181 ATTACTCGAGAGGACAACCATGGACTAGCCCTCGG<span class="snp"><tt>N</tt></span>CGTCTGGCCTC<span class="snp"><tt>N</tt></span>TATTGTGGAGCA 240
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
7470R-3.in silico     181 ATTACTCGAGAGGACAACCATGGACTAGCCCTCGG<span class="snp"><tt>C</tt></span>CGTCTGGCCTC<span class="snp"><tt>-</tt></span>TATTGTGGAGCA 240


7470R-3.IR_full       241 GGTTGCCGAGTGCCATTTGGAAGGACGTGAAGTGATGATGGTCACCAGCGGAGCGGTGGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7470R-3.in silico     241 GGTTGCCGAGTGCCATTTGGAAGGACGTGAAGTGATGATGGTCACCAGCGGAGCGGTGGC 300


7470R-3.IR_full       301 CT<span class="snp"><tt>N</tt></span>TTGGAAAGCAGAAGCTCGCCCAGGAGCTGCTCATGTCGCTGTCCATGCGCGAGACAC 360
                          ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7470R-3.in silico     301 CT<span class="snp"><tt>-</tt></span>TTGGAAAGCAGAAGCTCGCCCAGGAGCTGCTCATGTCGCTGTCCATGCGCGAGACAC 360


7470R-3.IR_full       361 TAAACCCAAAAGATAGCAAAGAGTTTGACGGCGCTACGCTGGAACCGCGAGCAGCGGCTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7470R-3.in silico     361 TAAACCCAAAAGATAGCAAAGAGTTTGACGGCGCTACGCTGGAACCGCGAGCAGCGGCTG 420


7470R-3.IR_full       421 CGGTGG<span class="snp"><tt>N</tt></span>GTCAGTCGGGTCTGATGTCCCTCTACGACGCGATGTTTGCCCAGTACGGCGTC 480
                          ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
7470R-3.in silico     421 CGGTGG<span class="snp"><tt>-</tt></span>GTCAGTCGGGTCTGATGTCCCTCTACGACGCGATGTTTGCCCAGTACGGCGTC 480


7470R-3.IR_full       481 AAGATAGCCCAGGTGCTCGTGAC 503
                          |||||||||||||||||||||||
7470R-3.in silico     481 AAGATAGCCCAGGTGCTCGTGAC 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141118.3	CG7470-RA (CG7470), mRNA
0	0	5	7	5	NM_165508.1	CG11084-RA, transcript variant A (pk), mRNA
0	0	5	7	5	NM_165512.1	CG11084-RC, transcript variant C (pk), mRNA
0	0	5	7	5	NM_165509.1	CG11084-RB, transcript variant B (pk), mRNA
0	0	3	3	3	NM_134804.3	CG7261-RA (CG7261), mRNA
0	0	3	2	3	NM_140919.1	CG7385-RA (CG7385), mRNA
0	0	1	2	5	NM_132010.1	CG17758-RA (CG17758), mRNA
0	0	1	2	2	NM_137591.1	CG15124-RA (CG15124), mRNA
0	0	0	8	5	NM_206381.1	CG16838-RC, transcript variant C (CG16838), mRNA
0	0	0	7	2	NM_206380.1	CG16838-RD, transcript variant D (CG16838), mRNA
0	0	0	6	5	NM_168881.1	CG32434-RB, transcript variant B (siz), mRNA
0	0	0	6	5	NM_206407.1	CG32434-RA, transcript variant A (siz), mRNA
0	0	0	6	5	NM_001043153.1	CG32434-RC, transcript variant C (siz), mRNA
0	0	0	5	5	NM_132492.1	CG1756-RA (CG1756), mRNA
0	0	0	5	4	NM_130645.2	CG14047-RA, transcript variant A (CG14047), mRNA
0	0	0	5	4	NM_206610.1	CG14047-RB, transcript variant B (CG14047), mRNA
0	0	0	5	2	NM_169051.1	CG31542-RA (CG31542), mRNA
0	0	0	4	11	NM_001014716.1	CG33513-RA, transcript variant A (Nmdar2), mRNA
0	0	0	4	11	NM_001014714.1	CG33513-RC, transcript variant C (Nmdar2), mRNA
0	0	0	4	11	NM_001014715.1	CG33513-RB, transcript variant B (Nmdar2), mRNA
0	0	0	4	10	NM_135344.2	CG8486-RA, transcript variant A (CG8486), mRNA
0	0	0	4	10	NM_164795.2	CG8486-RB, transcript variant B (CG8486), mRNA
0	0	0	4	10	NM_001042881.1	CG8486-RC, transcript variant C (CG8486), mRNA
0	0	0	4	8	NM_057882.3	CG1609-RA (Gcn2), mRNA
0	0	0	4	8	NM_167394.1	CG32603-RA (CG32603), mRNA
0	0	0	4	7	NM_078800.2	CG4422-RA (Gdi), mRNA
0	0	0	4	6	NM_137942.1	CG13550-RA (CG13550), mRNA
0	0	0	4	4	NM_136455.1	CG2093-RA (CG2093), mRNA
0	0	0	4	3	NM_080296.2	CG16785-RA (fz3), mRNA
0	0	0	4	2	NM_057502.2	CG1322-RB, transcript variant B (zfh1), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00330 : Arginine and proline metabolism
dme01100 : Metabolic pathways
dme01230 : Biosynthesis of amino acids

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