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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7464R-2 
 Symbol CS-2  Full Name Chitin synthase 2 
 CG No CG7464  Old CG No CG7464 
 Synonyms CG7464, ChSA, DmeChSA, DmCS-2, CS-2 
 Accession No (Link to NCBI)  
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
[Please submit your publication]
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42 [ PubMed ID = 25940448 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGACCACACAGACATGAGCGGA 
 Primer Seq. 3'
aatctagaggtaccACGTGCAGGAGTTCAAAGAGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
 
 in silico PCR Fragment
 
 Assemble Data

7464R-2.IR full       1   -ACCACACAGACATGAGCGGAGGAGACTACGATAGCGGTGACTACTTCATGCGCAGTCGG 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     1   GACCACACAGACATGAGCGGAGGAGACTACGATAGCGGTGACTACTTCATGCGCAGTCGG 60


7464R-2.IR full       61  AAGCAGCGGGACAAGCCGTCATTATGGGACTCCTTCCAGGACCCGCCGTCTAAGAAGACG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     61  AAGCAGCGGGACAAGCCGTCATTATGGGACTCCTTCCAGGACCCGCCGTCTAAGAAGACG 120


7464R-2.IR full       121 AGCGGATCCGATGCCGACTGGAAGAAGCTCCAGTTCTGCCTTAAGCTCTTCAAGATCTGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     121 AGCGGATCCGATGCCGACTGGAAGAAGCTCCAGTTCTGCCTTAAGCTCTTCAAGATCTGC 180


7464R-2.IR full       181 ACCTACCTCCTTGTCTTCGCGGTCGTTCTTGCGACGAGCGTGTTCGCCAACTTGATTTAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     181 ACCTACCTCCTTGTCTTCGCGGTCGTTCTTGCGACGAGCGTGTTCGCCAACTTGATTTAT 240


7464R-2.IR full       241 CTCTACATGGCCGGAAACACAGGTGGCACCGGGCCACGCGTGCAAGTGTGCAGCAAGTAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     241 CTCTACATGGCCGGAAACACAGGTGGCACCGGGCCACGCGTGCAAGTGTGCAGCAAGTAT 300


7464R-2.IR full       301 C---TACTNCNGCACGGCCAAGTGGAGGCGGTGGC-AGCGAGCTGGACCAAATCGCCTGG 360
                          |   |||| | |||||||||||||||||||||||| ||||||||||||||||||||||||
7464R-2.in silico     301 CCAGTACTCCGGCACGGCCAAGTGGAGGCGGTGGCCAGCGAGCTGGACCAAATCGCCTGG 360


7464R-2.IR full       361 GTCTGGGCCCTGATCTTCGCCTTTGCCGCCCCCGAGCTGCTCACCTTNCC-TGC-CNCTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||| |   || | |||
7464R-2.in silico     361 GTCTGGGCCCTGATCTTCGCCTTTGCCGCCCCCGAGCTGCTCACCTTCCTGCGCGCCCTG 420


7464R-2.IR full       421 CGCATCTGTATGTTCAAGCGCGAGCAGCGACCCAGCGGACTAGAAGTGGGACTGGTCACG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7464R-2.in silico     421 CGCATCTGTATGTTCAAGCGCGAGCAGCGACCCAGCGGACTAGAAGTGGGACTGGTCACG 480


7464R-2.IR full       481 CTCTTTGAACTCCTGCACGT 500
                          ||||||||||||||||||||
7464R-2.in silico     481 CTCTTTGAACTCCTGCACGT 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
 

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