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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7393R-1

Symbol p38b Full Name p38b

CG No CG7393 Old CG No CG7393

Synonyms p38b, p38beta, p38, Dp38, D-p38, D-p38b, p38 beta, p38B, CG7393, p38 MAPK, BG:DS00797.3, 186F5S, anon-sts23, Mpk34C, ESTS:186F5S, Dm p38b

Accession No (Link to NCBI) NM_058013.3

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal

Map Viewer

Reference
[Please submit your publication] Sun Y, Zhang D, Li C, Huang J, Li W, Qiu Y, Mao A, Zhou M, Xue L.
Lic regulates JNK-mediated cell death in Drosophila.
Cell Prolif (2019) 52(3) e12593 [ PubMed ID = 30847993 ] [ RRC reference ]

Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76 [ PubMed ID = 26551273 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Gonda RL, Garlena RA, Stronach B.
Drosophila heat shock response requires the JNK pathway and phosphorylation of mixed lineage kinase at a conserved serine-proline motif.
PLoS One (2012) 7(7) e42369 [ PubMed ID = 22848763 ] [ RRC reference ]

Sekine Y, Takagahara S, Hatanaka R, Watanabe T, Oguchi H, Noguchi T, Naguro I, Kobayashi K, Tsunoda M, Funatsu T, Nomura H, Toyoda T, Matsuki N, Kuranaga E, Miura M, Takeda K, Ichijo H.
p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors.
J Cell Sci (2011) 124(Pt 17) 3006-16 [ PubMed ID = 21878507 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCAAATTCTACAAGCTGGAC

Primer Seq. 3'

aatctagaggtaccAAATCCAGGATGCGAAGCTCA

Predicted Fragment Size

IR fragment full Seq

0001 GCCAAATTCT ACAAGCTGGA CATCAATCGC ACCGAGTGGG AAATCCCGGA AACATACCAG 
0061 AACCTGCAAC CCGTGGGTCA GGGTGCCTAC GgcCAGGTCT GCAAGGCCGT GGTCCGCGGA 
0121 ACCAGCACGA AGGTGGCCAT CAAGAAGCTT GCCAGGCCCT TCCAGTCGGC GGTCCATGCG 
0181 aAGCgNACCT ATCGGGAACT GCGGCTGNTG AAGCACATGG ATCACGAGAA CGTTATTGGT 
0241 CTGCTGGATG TCTTTCATCC AGGACAGCCC GCCGATTCGC TGGATCAGTT CCAGCAAGTG 
0301 TACATGGTGA CCCACTTGAT GGACGCCGAT CTGAACAACA TAATACGCAC GCAGAAACTG 
0361 TCTGATGATC ATGTCCAGTT TCTGGTCTAC CAAATCCTGC GCGGTCTGNN NTACATCCAC 
0421 AGCGCTGGGG TCATCCATCG TGATCTAAAG CCATCGAACA TTGCGGTAAA CGAGGANCTg 
0481 tgaGCTTCGC aTCCTGGGAT TT

in silico PCR Fragment

0001 GCCAAATTCT ACAAGCTGGA CATCAATCGC ACCGAGTGGG AAATCCCGGA AACATACCAG 
0061 AACCTGCAAC CCGTGGGTCA GGGTGCCTAC GGCCAGGTCT GCAAGGCCGT GGTCCGCGGA 
0121 ACCAGCACGA AGGTGGCCAT CAAGAAGCTT GCCAGGCCCT TCCAGTCGGC GGTCCATGCG 
0181 AAGCGCACCT ATCGGGAACT GCGGCTGCTG AAGCACATGG ATCACGAGAA CGTTATTGGT 
0241 CTGCTGGATG TCTTTCATCC AGGACAGCCC GCCGATTCGC TGGATCAGTT CCAGCAAGTG 
0301 TACATGGTGA CCCACTTGAT GGACGCCGAT CTGAACAACA TAATACGCAC GCAGAAACTG 
0361 TCTGATGATC ATGTCCAGTT TCTGGTCTAC CAAATCCTGC GCGGTCTGAA GTACATCCAC 
0421 AGCGCTGGGG TCATCCATCG TGATCTAAAG CCATCGAACA TTGCGGTAAA CGAGGA-CTG 
0481 TGAGCTTCGC ATCCT-GGAT TT

Assemble Data


7393R-1.IR_full       1   GCCAAATTCTACAAGCTGGACATCAATCGCACCGAGTGGGAAATCCCGGAAACATACCAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7393R-1.in silico     1   GCCAAATTCTACAAGCTGGACATCAATCGCACCGAGTGGGAAATCCCGGAAACATACCAG 60


7393R-1.IR_full       61  AACCTGCAACCCGTGGGTCAGGGTGCCTACGGCCAGGTCTGCAAGGCCGTGGTCCGCGGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7393R-1.in silico     61  AACCTGCAACCCGTGGGTCAGGGTGCCTACGGCCAGGTCTGCAAGGCCGTGGTCCGCGGA 120


7393R-1.IR_full       121 ACCAGCACGAAGGTGGCCATCAAGAAGCTTGCCAGGCCCTTCCAGTCGGCGGTCCATGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7393R-1.in silico     121 ACCAGCACGAAGGTGGCCATCAAGAAGCTTGCCAGGCCCTTCCAGTCGGCGGTCCATGCG 180


7393R-1.IR_full       181 AAGCGNACCTATCGGGAACTGCGGCTGNTGAAGCACATGGATCACGAGAACGTTATTGGT 240
                          ||||| ||||||||||||||||||||| ||||||||||||||||||||||||||||||||
7393R-1.in silico     181 AAGCGCACCTATCGGGAACTGCGGCTGCTGAAGCACATGGATCACGAGAACGTTATTGGT 240


7393R-1.IR_full       241 CTGCTGGATGTCTTTCATCCAGGACAGCCCGCCGATTCGCTGGATCAGTTCCAGCAAGTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7393R-1.in silico     241 CTGCTGGATGTCTTTCATCCAGGACAGCCCGCCGATTCGCTGGATCAGTTCCAGCAAGTG 300


7393R-1.IR_full       301 TACATGGTGACCCACTTGATGGACGCCGATCTGAACAACATAATACGCACGCAGAAACTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7393R-1.in silico     301 TACATGGTGACCCACTTGATGGACGCCGATCTGAACAACATAATACGCACGCAGAAACTG 360


7393R-1.IR_full       361 TCTGATGATCATGTCCAGTTTCTGGTCTACCAAATCCTGCGCGGTCTGNNNTACATCCAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||   |||||||||
7393R-1.in silico     361 TCTGATGATCATGTCCAGTTTCTGGTCTACCAAATCCTGCGCGGTCTGAAGTACATCCAC 420


7393R-1.IR_full       421 AGCGCTGGGGTCATCCATCGTGATCTAAAGCCATCGAACATTGCGGTAAACGAGGANCTG 480
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
7393R-1.in silico     421 AGCGCTGGGGTCATCCATCGTGATCTAAAGCCATCGAACATTGCGGTAAACGAGGA-CTG 480


7393R-1.IR_full       481 TGAGCTTCGCATCCTGGGATTT 502
                          ||||||||||||||| ||||||
7393R-1.in silico     481 TGAGCTTCGCATCCT-GGATTT 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_058013.3	CG7393-RA (p38b), mRNA
0.2	1	2	5	3	NM_137810.1	CG3290-RA (CG3290), mRNA
0	0	21	48	95	NM_170126.2	CG5475-RB, transcript variant B (Mpk2), mRNA
0	0	21	48	95	NM_057815.3	CG5475-RA, transcript variant A (Mpk2), mRNA
0	0	2	12	34	NM_206554.1	CG33338-RA (p38c), mRNA
0	0	2	8	9	NM_142727.1	CG6800-RA (CG6800), mRNA
0	0	2	2	3	NM_176581.2	CG33204-RA (CG33204), mRNA
0	0	1	7	3	NM_166760.1	CG1495-RB, transcript variant B (CaMKI), mRNA
0	0	1	7	3	NM_166761.1	CG1495-RC, transcript variant C (CaMKI), mRNA
0	0	1	7	3	NM_166759.1	CG1495-RA, transcript variant A (CaMKI), mRNA
0	0	1	7	3	NM_166762.1	CG1495-RE, transcript variant E (CaMKI), mRNA
0	0	1	7	3	NM_079883.2	CG1495-RG, transcript variant G (CaMKI), mRNA
0	0	1	7	3	NM_166764.1	CG1495-RH, transcript variant H (CaMKI), mRNA
0	0	1	7	3	NM_166763.1	CG1495-RD, transcript variant D (CaMKI), mRNA
0	0	1	5	11	NM_166415.1	CG3216-RB, transcript variant B (CG3216), mRNA
0	0	1	5	11	NM_137688.1	CG3216-RA, transcript variant A (CG3216), mRNA
0	0	1	5	6	NM_165298.1	CG10237-RC, transcript variant C (CG10237), mRNA
0	0	1	5	6	NM_136123.2	CG10237-RB, transcript variant B (CG10237), mRNA
0	0	1	5	6	NM_165299.1	CG10237-RA, transcript variant A (CG10237), mRNA
0	0	1	5	3	NM_167082.2	CG4095-RA (CG4095), mRNA
0	0	1	4	15	NM_137515.1	CG15072-RA (CG15072), mRNA
0	0	1	3	3	NM_140580.1	CG5414-RA (CG5414), mRNA
0	0	1	2	8	NM_170365.1	CG14066-RC, transcript variant C (larp), mRNA
0	0	1	2	8	NM_170366.1	CG14066-RB, transcript variant B (larp), mRNA
0	0	1	2	8	NM_080259.1	CG14066-RA, transcript variant A (larp), mRNA
0	0	1	2	5	NM_137811.2	CG3264-RA (CG3264), mRNA
0	0	1	2	5	NM_134904.2	CG3523-RA (CG3523), mRNA
0	0	1	2	3	NM_165970.1	CG4062-RB, transcript variant B (Aats-val), mRNA
0	0	1	2	3	NM_080099.2	CG4062-RA, transcript variant A (Aats-val), mRNA
0	0	1	2	3	NR_002550.1	CR33655, miscRNA

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