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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7103R-1 |
| Symbol |
Pvf1 |
Full Name |
PDGF- and VEGF-related factor 1 |
| CG No |
CG7103 |
Old CG No |
CG7103 |
| Synonyms |
vegf1, PVF, PVF1, CG7103, PVFs, pvf1, Vegf17e-b, VEGF2-B, Vegf17e-a, Vegf17E, VEGF, DmVEGF-1, unnamed, l(1)G0146, EP1624, Pvf1 |
| Accession No (Link to NCBI) |
NM_078683.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Chen Y, Xu W, Chen Y, Han A, Song J, Zhou X, Song W.
Renal NF-κB activation impairs uric acid homeostasis to promote tumor-associated mortality independent of wasting.
Immunity (2022) 55(9) 1594-1608.e6
[
PubMed ID = 36029766
]
[
RRC reference
]
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Song W, Kir S, Hong S, Hu Y, Wang X, Binari R, Tang HW, Chung V, Banks AS, Spiegelman B, Perrimon N.
Tumor-Derived Ligands Trigger Tumor Growth and Host Wasting via Differential MEK Activation.
Dev Cell (2019) 48(2) 277-286.e6
[
PubMed ID = 30639055
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Garlena RA, Lennox AL, Baker LR, Parsons TE, Weinberg SM, Stronach BE.
The receptor tyrosine kinase Pvr promotes tissue closure by coordinating corpse removal and epidermal zippering.
Development (2015) 142(19) 3403-15
[
PubMed ID = 26293306
]
[
RRC reference
]
|
| Comment |
Lost by accident. Kindly donated by Dr. Stronach.
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCATTGCTCATCCTGCCAGTG |
| Primer Seq. 3' |
aatctagaggtaccGTGGATCCGCAGCATCCATTG |
| Predicted Fragment Size |
497 |
| IR fragment full Seq |
0001 CCATTGCTCA TCCTGCCAGT GCTCCTTCTC GTCCTGATCG TCAGCGAGGC AGCGGCGGGC
0061 TCCTTGGTGT CCCCGAACAA CAGGCAACCA TCGCAGAGGT TCTTCTACGC CGCCGCCACG
0121 TCCTCCTCCT CCACGCAGAC CAAGGTGCGC CTGCTCCGCC AGgcGGACGA TCCCAGTGCG
0181 GCGCCGGGTG CACTGGAGGG CAGCTGCTGC CAAGGAGCAG CTGCGGAAGC CGGAACACCA
0241 TTGACGCTCC CACTGTCCGT GTCCGCTGAG CTGACCAACG TGACCGCCGA CTACGATGTG
0301 TCCGGCGAGA TGCCCTCCTC CAAGGAAAAC TTTAAGCGCA GCATCATGAA ATCAACGGTG
0361 AGGAATGCAA CGCCGGCGAG CTGCTCCCCG CAACCGACGA TCGTGGAGCT GAAGCCACCG
0421 GCGGAGGACG AGGCGAACTA CTACTATATG CCCGCGTGCA CGCGGATCAG CCGATGCAAT
0481 GGATGCTGCG GATCCAC |
| in silico PCR Fragment |
0001 CCATTGCTCA TCCTGCCAGT GCTCCTTCTC GTCCTGATCG TCAGCGAGGC AGCGGCGGGC
0061 TCCTTGGTGT CCCCGAACAA CAGGCAACCA TCGCAGAGGT TCTTCTACGC CGCCGCCACG
0121 TCCTCCTCCT CCACGCAGAC CAAGGTGCGC CTGCTCCGCC AGGCGGACGA TCCCAGTGCG
0181 GCGCCGGGTG CACTGGAGGG CAGCTGCTGC CAAGGAGCAG CTGCGGAAGC CGGAACACCA
0241 TTGACGCTCC CACTGTCCGT GTCCGCTGAG CTGACCAACG TGACCGCCGA CTACGATGTG
0301 TCCGGCGAGA TGCCCTCCTC CAAGGAAAAC TTTAAGCGCA GCATCATGAA ATCAACCGTG
0361 AGGAATGCAA CGCCGGCGAG CTGCTCCCCG CAACCGACGA TCGTGGAGCT GAAGCCACCG
0421 GCGGAGGACG AGGCGAACTA CTACTATATG CCCGCGTGCA CGCGGATCAG CCGATGCAAT
0481 GGATGCTGCG GATCCAC |
| Assemble Data |
7103R-1.IR_full 1 CCATTGCTCATCCTGCCAGTGCTCCTTCTCGTCCTGATCGTCAGCGAGGCAGCGGCGGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 1 CCATTGCTCATCCTGCCAGTGCTCCTTCTCGTCCTGATCGTCAGCGAGGCAGCGGCGGGC 60
7103R-1.IR_full 61 TCCTTGGTGTCCCCGAACAACAGGCAACCATCGCAGAGGTTCTTCTACGCCGCCGCCACG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 61 TCCTTGGTGTCCCCGAACAACAGGCAACCATCGCAGAGGTTCTTCTACGCCGCCGCCACG 120
7103R-1.IR_full 121 TCCTCCTCCTCCACGCAGACCAAGGTGCGCCTGCTCCGCCAGGCGGACGATCCCAGTGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 121 TCCTCCTCCTCCACGCAGACCAAGGTGCGCCTGCTCCGCCAGGCGGACGATCCCAGTGCG 180
7103R-1.IR_full 181 GCGCCGGGTGCACTGGAGGGCAGCTGCTGCCAAGGAGCAGCTGCGGAAGCCGGAACACCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 181 GCGCCGGGTGCACTGGAGGGCAGCTGCTGCCAAGGAGCAGCTGCGGAAGCCGGAACACCA 240
7103R-1.IR_full 241 TTGACGCTCCCACTGTCCGTGTCCGCTGAGCTGACCAACGTGACCGCCGACTACGATGTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 241 TTGACGCTCCCACTGTCCGTGTCCGCTGAGCTGACCAACGTGACCGCCGACTACGATGTG 300
7103R-1.IR_full 301 TCCGGCGAGATGCCCTCCTCCAAGGAAAACTTTAAGCGCAGCATCATGAAATCAACGGTG 360
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7103R-1.in silico 301 TCCGGCGAGATGCCCTCCTCCAAGGAAAACTTTAAGCGCAGCATCATGAAATCAACCGTG 360
7103R-1.IR_full 361 AGGAATGCAACGCCGGCGAGCTGCTCCCCGCAACCGACGATCGTGGAGCTGAAGCCACCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 361 AGGAATGCAACGCCGGCGAGCTGCTCCCCGCAACCGACGATCGTGGAGCTGAAGCCACCG 420
7103R-1.IR_full 421 GCGGAGGACGAGGCGAACTACTACTATATGCCCGCGTGCACGCGGATCAGCCGATGCAAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7103R-1.in silico 421 GCGGAGGACGAGGCGAACTACTACTATATGCCCGCGTGCACGCGGATCAGCCGATGCAAT 480
7103R-1.IR_full 481 GGATGCTGCGGATCCAC 497
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7103R-1.in silico 481 GGATGCTGCGGATCCAC 497
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
479 |
0 |
0 |
2 |
NM_078683.2 |
CG7103-RA (Pvf1), mRNA |
| 0.2 |
1 |
4 |
2 |
4 |
NM_137980.2 |
CG12491-RA (CG12491), mRNA |
| 0.2 |
1 |
4 |
2 |
4 |
NM_001043102.1 |
CG34105-RA (CG34105), mRNA |
| 0.2 |
1 |
2 |
10 |
14 |
NM_079093.2 |
CG9888-RA (Fib), mRNA |
| 0 |
0 |
7 |
2 |
4 |
NM_135302.3 |
CG7191-RA (CG7191), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_133125.2 |
CG7537-RB (inx5), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_141038.2 |
CG9389-RA (CG9389), mRNA |
| 0 |
0 |
2 |
8 |
10 |
NM_141334.1 |
CG10979-RA (CG10979), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_143044.2 |
CG7016-RA (CG7016), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_169121.1 |
CG10279-RF, transcript variant F (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_169122.1 |
CG10279-RB, transcript variant B (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_079519.2 |
CG10279-RA, transcript variant A (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_169120.1 |
CG10279-RC, transcript variant C (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_169118.1 |
CG10279-RD, transcript variant D (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_169119.1 |
CG10279-RE, transcript variant E (Rm62), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_079163.2 |
CG1960-RA, transcript variant A (mu2), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_167927.1 |
CG1960-RB, transcript variant B (mu2), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132404.1 |
CG2889-RA (CG2889), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_206789.1 |
CG33253-RA (CG33253), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_131982.1 |
CG3323-RA (CG3323), mRNA |
| 0 |
0 |
2 |
2 |
16 |
NM_169489.1 |
CG7518-RA, transcript variant A (CG7518), mRNA |
| 0 |
0 |
2 |
2 |
16 |
NM_141993.2 |
CG7518-RB, transcript variant B (CG7518), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_137909.2 |
CG9882-RA (Art7), mRNA |
| 0 |
0 |
1 |
10 |
13 |
NM_141666.2 |
CG9492-RA (CG9492), mRNA |
| 0 |
0 |
1 |
8 |
30 |
NM_057695.3 |
CG4038-RA (CG4038), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_169654.2 |
CG31302-RC, transcript variant C (CG31302), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_169652.2 |
CG31302-RA, transcript variant A (CG31302), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_169653.2 |
CG31302-RB, transcript variant B (CG31302), mRNA |
|
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