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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7100R-3 
 Symbol CadN  Full Name Cadherin-N 
 CG No CG7100  Old CG No CG7100 
 Synonyms Ncad, N-cad, N-Cad, S(DmcycE[JP])2.12, ncad, CT21941, DN-Cad, DN-cad, DN, l(2)36Da, B, D-cad, l(2)Bld, NCad, anon-EST:CL32, CG7100, CadN, cad1, cadN 
 Accession No (Link to NCBI) NM_165229.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem. Biophys. Res. Commun. (2014) 451(4) 548-55 [ PubMed ID = 25117438 ] [ RRC reference ]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, D├╝rrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev. Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGAATCAGCTCAGGCAACGTTA 
 Primer Seq. 3'
aatctagaggtaccGGGATCCAGAAAATCGCAGCA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 GAATCAGCTC AGGCAACGTT ATATAACCAA CCGATTTAAT ATATGCACCT GCGCGATATT 
0061 CTTGATATCC CTTCCATTTA TTTTGGCCAT AGAGGAGACC ACATTTGCTG GTCTTTCGGC 
0121 GGAAAATGCG GCTCGCATGC TGGCCGGCAG TCCCGGGGAT GTGGAAAAGT CCTCGTTGTC 
0181 GCATCACAGT GAAATGTCCT TGGTCCTGCC ACACGATACC TATCCTGGAT TCAGTATCAA 
0241 AAAGTTCAAA ACCCATCCTG TAAAGATAAA CGGTAGCTCC CACTCTGGTG CAGCTGCCTA 
0301 TCACATGTTG GACACGGACT ACTCCAAGTA CTTCACCGTA CTGGAAGATG GTGTGGTGAT 
0361 GACCACGGCG GATATTTCCC CGCTTGTCAA TCGTCCGGTT CAGTTGGTGG TTGTTGAACA 
0421 AACGCCCAAT GCCACCAATA CCCACAATCT GCAGCTGTTT GTGATGCACC GCAATGATAT 
0481 GCTGCGATTT TCTGGATCCC  
 in silico PCR Fragment
0001 GAATCAGCTC AGGCAACGTT ATATAACCAA CCGATTTAAT ATATGCACCT GCGCGATATT 
0061 CTTGATATCC CTTCCATTTA TTTTGGCCAT AGAGGAGACC ACATTTGCTG GTCTTTCGGC 
0121 GGAAAATGCG GCTCGCATGC TGGCCGGCAG TCCCGGGGAT GTGGAAAAGT CCTCGTTGTC 
0181 GCATCACAGT GAAATGTCCT TGGTCCTGCC ACACGATACC TATCCTGGAT TCAGTATCAA 
0241 AAAGTTCAAA ACCCATCCTG TAAAGATAAA CGGTAGCTCC CACTCTGGTG CAGCTGCCTA 
0301 TCACATGTTG GACACGGACT ACTCCAAGTA CTTCACCGTA CTGGAAGATG GTGTGGTGAT 
0361 GACCACGGCG GATATTTCCC CGCTTGTCAA TCGTCCGGTT CAGTTGGTGG TTGTTGAACA 
0421 AACGCCCAAT GCCACCAATA CCCACAATCT GCAGCTGTTT GTGATGCACC GCAATGATAT 
0481 GCTGCGATTT TCTGGATCCC  
 Assemble Data

7100R-3.IR_full       1   GAATCAGCTCAGGCAACGTTATATAACCAACCGATTTAATATATGCACCTGCGCGATATT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     1   GAATCAGCTCAGGCAACGTTATATAACCAACCGATTTAATATATGCACCTGCGCGATATT 60


7100R-3.IR_full       61  CTTGATATCCCTTCCATTTATTTTGGCCATAGAGGAGACCACATTTGCTGGTCTTTCGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     61  CTTGATATCCCTTCCATTTATTTTGGCCATAGAGGAGACCACATTTGCTGGTCTTTCGGC 120


7100R-3.IR_full       121 GGAAAATGCGGCTCGCATGCTGGCCGGCAGTCCCGGGGATGTGGAAAAGTCCTCGTTGTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     121 GGAAAATGCGGCTCGCATGCTGGCCGGCAGTCCCGGGGATGTGGAAAAGTCCTCGTTGTC 180


7100R-3.IR_full       181 GCATCACAGTGAAATGTCCTTGGTCCTGCCACACGATACCTATCCTGGATTCAGTATCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     181 GCATCACAGTGAAATGTCCTTGGTCCTGCCACACGATACCTATCCTGGATTCAGTATCAA 240


7100R-3.IR_full       241 AAAGTTCAAAACCCATCCTGTAAAGATAAACGGTAGCTCCCACTCTGGTGCAGCTGCCTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     241 AAAGTTCAAAACCCATCCTGTAAAGATAAACGGTAGCTCCCACTCTGGTGCAGCTGCCTA 300


7100R-3.IR_full       301 TCACATGTTGGACACGGACTACTCCAAGTACTTCACCGTACTGGAAGATGGTGTGGTGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     301 TCACATGTTGGACACGGACTACTCCAAGTACTTCACCGTACTGGAAGATGGTGTGGTGAT 360


7100R-3.IR_full       361 GACCACGGCGGATATTTCCCCGCTTGTCAATCGTCCGGTTCAGTTGGTGGTTGTTGAACA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     361 GACCACGGCGGATATTTCCCCGCTTGTCAATCGTCCGGTTCAGTTGGTGGTTGTTGAACA 420


7100R-3.IR_full       421 AACGCCCAATGCCACCAATACCCACAATCTGCAGCTGTTTGTGATGCACCGCAATGATAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7100R-3.in silico     421 AACGCCCAATGCCACCAATACCCACAATCTGCAGCTGTTTGTGATGCACCGCAATGATAT 480


7100R-3.IR_full       481 GCTGCGATTTTCTGGATCCC 500
                          ||||||||||||||||||||
7100R-3.in silico     481 GCTGCGATTTTCTGGATCCC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_001032106.1  CG7100-RL, transcript variant L (CadN), mRNA 
100   482  NM_001032107.1  CG7100-RK, transcript variant K (CadN), mRNA 
100   482  NM_001032109.1  CG7100-RI, transcript variant I (CadN), mRNA 
100   482  NM_001032108.1  CG7100-RJ, transcript variant J (CadN), mRNA 
100   482  NM_165231.1  CG7100-RE, transcript variant E (CadN), mRNA 
100   482  NM_165230.1  CG7100-RG, transcript variant G (CadN), mRNA 
100   482  NM_165229.1  CG7100-RA, transcript variant A (CadN), mRNA 
100   482  NM_165228.1  CG7100-RC, transcript variant C (CadN), mRNA 
100   482  NM_165227.1  CG7100-RD, transcript variant D (CadN), mRNA 
100   482  NM_165226.1  CG7100-RF, transcript variant F (CadN), mRNA 
100   482  NM_165225.1  CG7100-RB, transcript variant B (CadN), mRNA 
100   482  NM_165224.1  CG7100-RH, transcript variant H (CadN), mRNA 
0   NM_001043212.1  CG12591-RB, transcript variant B (dpr16), mRNA 
0   NM_141257.1  CG12591-RA, transcript variant A (dpr16), mRNA 
0   NM_136801.1  CG13228-RA (CG13228), mRNA 
0   NM_139371.1  CG13917-RA (CG13917), mRNA 
0   NM_131938.1  CG3568-RA (CG3568), mRNA 
0   NM_058145.3  CG2707-RA (fs(1)Ya), mRNA 
0   NM_134711.1  CG15824-RA (CG15824), mRNA 
0   NM_079609.2  CG6019-RA (mus308), mRNA 
0   NM_142031.2  CG17045-RA (yellow-e3), mRNA 
0   NM_132050.1  CG3011-RA (CG3011), mRNA 
0   NM_140298.2  CG6906-RA (CAH2), mRNA 
0   NM_141495.2  CG2791-RA (CG2791), mRNA 
0   NM_001042855.1  CG40305-RA (FucTC), mRNA 
0   NM_134494.2  CG12235-RA (Arp11), mRNA 
0   NM_079818.2  CG10002-RA (fkh), mRNA 
0   NM_079766.2  CG7005-RA (Esp), mRNA 
0   NM_168361.1  CG16711-RB, transcript variant B (CG16711), mRNA 
0   NM_140090.1  CG16711-RA, transcript variant A (CG16711), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
DC
++ 
DM
VM
VB
 
Dcompartment   Penetrance
     

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