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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7069R-1

Symbol

CG7069

Full Name

CG7069

CG No

CG7069

Old CG No

CG7069

Synonyms

CG7069

Accession No (Link to NCBI)

NM_142773.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

abnormal wing/mesonotum

Map Viewer

Reference
[Please submit your publication]

Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311 [ PubMed ID = 34551427 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCTCACGGCTCTCACGAGTAT

Primer Seq. 3'

aatctagaggtaccGCGAAGATCATGTCCACCTTT

Predicted Fragment Size

IR fragment full Seq

0001 TCTCACgGCT CTCACGAGTA TCACTGCCAG ACAATACAGG CGGCTCGTAA GGCCATCGCC 
0061 ATGTACGTGG AGCAAACGGG TCTGCCCAGA ACATTGGCAA TTGCTCTGGA CACCAAGGGT 
0121 CCGGAGATCC GGACGGGCAA ACTGGCCGGT GGCAATGATA GGGCTGAGAT AGAGCTCAAG 
0181 ACCGGAGACA AAGTGACATT GAGCACCAAG AAGGAGATGG CAGACAAGAG CACCAAGGAT 
0241 AATATCTATG TGGATTATCA GAGGCTACCT CAACTGGTCA AACCAGGAAA TCGGGTATTT 
0301 GTAGACGACG GATTGATTGC ATTGATCGTG AAGGAATCAA AGGGAGATGA AGTGATCTGT 
0361 CAGGTGGAGA ACGGTGGCAA GCTGGGCTCC CACAAGGGAA TCAATTTGCC AGGAGTTCCG 
0421 GTAGATCTTC CCAGCGTAAC GGAGAAGGAT AAGCAGGATC TTAAATTTGG GGCCGAGCAA 
0481 AAGGTGGACA TGATCTTCGC

in silico PCR Fragment

0001 TCTCACGGCT CTCACGAGTA TCACTGCCAG ACAATACAGG CGGCTCGTAA GGCCATCGCC 
0061 ATGTACGTGG AGCAAACGGG TCTGCCCAGA ACATTGGCAA TTGCTCTGGA CACCAAGGGT 
0121 CCAGAGATCC GGACGGGCAA ACTGGCCGGT GGCAATGATA GGGCTGAGAT AGAGCTCAAG 
0181 ACCGGAGACA AAGTGACATT GAGCACCAAG AAGGAGATGG CAGACAAGAG TAACAAGGAT 
0241 AATATCTATG TGGATTATCA GAGGCTACCT CAACTGGTCA AACCAGGAAA TCGGGTATTT 
0301 GTAGACGACG GATTGATTGC ATTGATCGTG AAGGAATCAA AGGGAGATGA AGTGATCTGT 
0361 CAGGTGGAGA ACGGTGGCAA GCTGGGCTCC CACAAGGGAA TCAATTTGCC AGGAGTTCCG 
0421 GTAGATCTTC CCAGCGTAAC GGAGAAGGAT AAGCAGGATC TTAAATTTGG GGCCGAGCAA 
0481 AAGGTGGACA TGATCTTCGC

Assemble Data

<PRE>

7069R-1.IR_full       1   TCTCACGGCTCTCACGAGTATCACTGCCAGACAATACAGGCGGCTCGTAAGGCCATCGCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     1   TCTCACGGCTCTCACGAGTATCACTGCCAGACAATACAGGCGGCTCGTAAGGCCATCGCC 60


7069R-1.IR_full       61  ATGTACGTGGAGCAAACGGGTCTGCCCAGAACATTGGCAATTGCTCTGGACACCAAGGGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     61  ATGTACGTGGAGCAAACGGGTCTGCCCAGAACATTGGCAATTGCTCTGGACACCAAGGGT 120


7069R-1.IR_full       121 CC<span class="snp"><tt>G</tt></span>GAGATCCGGACGGGCAAACTGGCCGGTGGCAATGATAGGGCTGAGATAGAGCTCAAG 180
                          ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     121 CC<span class="snp"><tt>A</tt></span>GAGATCCGGACGGGCAAACTGGCCGGTGGCAATGATAGGGCTGAGATAGAGCTCAAG 180


7069R-1.IR_full       181 ACCGGAGACAAAGTGACATTGAGCACCAAGAAGGAGATGGCAGACAAGAG<span class="snp"><tt>C</tt></span>A<span class="snp"><tt>C</tt></span>CAAGGAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||
7069R-1.in silico     181 ACCGGAGACAAAGTGACATTGAGCACCAAGAAGGAGATGGCAGACAAGAG<span class="snp"><tt>T</tt></span>A<span class="snp"><tt>A</tt></span>CAAGGAT 240


7069R-1.IR_full       241 AATATCTATGTGGATTATCAGAGGCTACCTCAACTGGTCAAACCAGGAAATCGGGTATTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     241 AATATCTATGTGGATTATCAGAGGCTACCTCAACTGGTCAAACCAGGAAATCGGGTATTT 300


7069R-1.IR_full       301 GTAGACGACGGATTGATTGCATTGATCGTGAAGGAATCAAAGGGAGATGAAGTGATCTGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     301 GTAGACGACGGATTGATTGCATTGATCGTGAAGGAATCAAAGGGAGATGAAGTGATCTGT 360


7069R-1.IR_full       361 CAGGTGGAGAACGGTGGCAAGCTGGGCTCCCACAAGGGAATCAATTTGCCAGGAGTTCCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     361 CAGGTGGAGAACGGTGGCAAGCTGGGCTCCCACAAGGGAATCAATTTGCCAGGAGTTCCG 420


7069R-1.IR_full       421 GTAGATCTTCCCAGCGTAACGGAGAAGGATAAGCAGGATCTTAAATTTGGGGCCGAGCAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7069R-1.in silico     421 GTAGATCTTCCCAGCGTAACGGAGAAGGATAAGCAGGATCTTAAATTTGGGGCCGAGCAA 480


7069R-1.IR_full       481 AAGGTGGACATGATCTTCGC 500
                          ||||||||||||||||||||
7069R-1.in silico     481 AAGGTGGACATGATCTTCGC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_142773.1	CG7069-RA (CG7069), mRNA
0.2	1	2	2	4	NM_078712.2	CG12794-RA (lcs), mRNA
0	0	22	23	36	NM_142131.1	CG7362-RA (CG7362), mRNA
0	0	3	2	2	NM_143598.1	CG15564-RA (CG15564), mRNA
0	0	2	19	26	NM_079724.2	CG7070-RA, transcript variant A (PyK), mRNA
0	0	2	19	26	NM_170004.1	CG7070-RB, transcript variant B (PyK), mRNA
0	0	2	4	3	NM_166962.1	CG32498-RC, transcript variant C (dnc), mRNA
0	0	2	4	3	NM_166964.1	CG32498-RD, transcript variant D (dnc), mRNA
0	0	2	4	3	NM_166963.1	CG32498-RK, transcript variant K (dnc), mRNA
0	0	2	4	3	NM_166965.1	CG32498-RJ, transcript variant J (dnc), mRNA
0	0	2	4	3	NM_166961.1	CG32498-RI, transcript variant I (dnc), mRNA
0	0	1	5	6	NM_142762.2	CG7080-RA (CG7080), mRNA
0	0	1	4	6	NM_143504.2	CG7912-RA (CG7912), mRNA
0	0	1	4	4	NM_133149.2	CG8051-RA (CG8051), mRNA
0	0	1	3	2	NM_140821.2	CG3819-RA (CG3819), mRNA
0	0	1	3	2	NM_132773.2	CG5627-RA (rab3-GEF), mRNA
0	0	1	2	5	NM_164966.2	CG6464-RA (salm), mRNA
0	0	1	2	4	NM_164354.1	CG31973-RB, transcript variant B (CG31973), mRNA
0	0	1	2	3	NM_057849.3	CG3733-RA (Chd1), mRNA
0	0	1	2	2	NM_164353.1	CG31973-RA, transcript variant A (CG31973), mRNA
0	0	1	2	2	NM_001042861.1	CG31973-RC, transcript variant C (CG31973), mRNA
0	0	0	8	2	NM_131930.1	CG15572-RA (CG15572), mRNA
0	0	0	4	23	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	4	9	NM_140314.2	CG10698-RA (GRHRII), mRNA
0	0	0	4	7	NM_057269.2	CG10236-RA (LanA), mRNA
0	0	0	4	5	NM_144372.1	CG13270-RA (Ugt36Ba), mRNA
0	0	0	4	5	NM_079764.2	CG6875-RA (asp), mRNA
0	0	0	4	4	NM_143195.1	CG14541-RA (CG14541), mRNA
0	0	0	4	4	NM_139475.1	CG16986-RA (CG16986), mRNA
0	0	0	4	4	NM_139532.1	CG14967-RA (CG14967), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00010 : Glycolysis / Gluconeogenesis
dme00620 : Pyruvate metabolism
dme01100 : Metabolic pathways
dme01200 : Carbon metabolism
dme01230 : Biosynthesis of amino acids

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