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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

7033R-1

Symbol

CG7033

Full Name

CG7033

CG No

CG7033

Old CG No

CG7033

Synonyms

SD02216p, CG7033

Accession No (Link to NCBI)

NM_132296.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Kim AR, Choi KW.<br> TRiC/CCT chaperonins are essential for organ growth by interacting with insulin/TOR signaling in Drosophila.<br> Oncogene (2019) 38(24) 4739-4754 [ PubMed ID = 30792539 ] [ RRC reference ]

Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121 [ PubMed ID = 31034473 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCCGTCTGTCCTCGTTCATTG

Primer Seq. 3'

aatctagaggtaccACGCATCACAGCATCCACCGC

Predicted Fragment Size

IR fragment full Seq

0001 CCCGTCTGTC CTCGTTCATT GGTGCCATCG CCATTGGTGA TCTGGTAAAG AGCACGCTGG 
0061 GTCCGAAGGG TATGGACAAG ATCCTGGTGG CCACCGGACG CAACGCCGGT CATGTTGAGG 
0121 TGACCAACGA TGGTGCCACC ATTCTGCGTG CCGTCGGTGT AGACAATCCG GCCGCCAAGA 
0181 TTCTGGTCGA TATGTCGCGT GTGCAGGACG AGGAAGTGGG CGATGGCACC ACCTCGGTCA 
0241 CCGTTCTGGC ATCGGAACTG CTGCGCGAGG CGGAGNAAAC TAGTCGAACA GAAGCTGCAC 
0301 CCACAGATCA TCGTCNTCTG GCTGGCGTCA AGCCACCCAG GNTGGCACTG GAGGCGCTCA 
0361 CCACCGCCGC TCAGGACAAC TCGTCCAGTG ACGAGAAGTT CCGCAACGAC CTGCTGAACA 
0421 TTGCCCGCAC CACGCTCTCC TCGAAGATCC TGCACCAGCA CAAAGACTTC TTCGCCAATC 
0481 TGGCGGTGGA TGCTGTGATG CGT

in silico PCR Fragment

0001 CCCGTCTGTC CTCGTTCATT GGTGCCATCG CCATTGGTGA TCTGGTAAAG AGCACGCTGG 
0061 GTCCGAAGGG TATGGACAAG ATCCTGGTGG CCACCGGACG CAACGCCGGT CATGTTGAGG 
0121 TGACCAACGA TGGTGCCACC ATTCTGCGTG CCGTCGGTGT AGACAATCCG GCCGCCAAGA 
0181 TTCTGGTCGA TATGTCGCGT GTGCAGGACG AGGAAGTGGG CGATGGCACC ACCTCGGTCA 
0241 CCGTTCTGGC ATCGGAACTG CTGCGCGAGG CGGAG-AAAC TAGTCGAACA GAAGCTGCAC 
0301 CCACAGATCA TCGTC-TCTG GCTGGCGTCA GGCCACCCAG G-TGGCACTG GAGGCGCTCA 
0361 CCGCCGCCGC TCAGGACAAC TCGTCCAGTG ACGAGAAGTT CCGCAACGAC CTGCTGAACA 
0421 TTGCCCGCAC CACGCTCTCC TCGAAGATCC TGCACCAGCA CAAAGACTTC TTCGCCAATC 
0481 TGGCGGTGGA TGCTGTGATG CGT

Assemble Data

<PRE>

7033R-1.IR_full       1   CCCGTCTGTCCTCGTTCATTGGTGCCATCGCCATTGGTGATCTGGTAAAGAGCACGCTGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     1   CCCGTCTGTCCTCGTTCATTGGTGCCATCGCCATTGGTGATCTGGTAAAGAGCACGCTGG 60


7033R-1.IR_full       61  GTCCGAAGGGTATGGACAAGATCCTGGTGGCCACCGGACGCAACGCCGGTCATGTTGAGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     61  GTCCGAAGGGTATGGACAAGATCCTGGTGGCCACCGGACGCAACGCCGGTCATGTTGAGG 120


7033R-1.IR_full       121 TGACCAACGATGGTGCCACCATTCTGCGTGCCGTCGGTGTAGACAATCCGGCCGCCAAGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     121 TGACCAACGATGGTGCCACCATTCTGCGTGCCGTCGGTGTAGACAATCCGGCCGCCAAGA 180


7033R-1.IR_full       181 TTCTGGTCGATATGTCGCGTGTGCAGGACGAGGAAGTGGGCGATGGCACCACCTCGGTCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     181 TTCTGGTCGATATGTCGCGTGTGCAGGACGAGGAAGTGGGCGATGGCACCACCTCGGTCA 240


7033R-1.IR_full       241 CCGTTCTGGCATCGGAACTGCTGCGCGAGGCGGAG<span class="snp"><tt>N</tt></span>AAACTAGTCGAACAGAAGCTGCAC 300
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
7033R-1.in silico     241 CCGTTCTGGCATCGGAACTGCTGCGCGAGGCGGAG<span class="snp"><tt>-</tt></span>AAACTAGTCGAACAGAAGCTGCAC 300


7033R-1.IR_full       301 CCACAGATCATCGTC<span class="snp"><tt>N</tt></span>TCTGGCTGGCGTCA<span class="snp"><tt>A</tt></span>GCCACCCAGG<span class="snp"><tt>N</tt></span>TGGCACTGGAGGCGCTCA 360
                          |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
7033R-1.in silico     301 CCACAGATCATCGTC<span class="snp"><tt>-</tt></span>TCTGGCTGGCGTCA<span class="snp"><tt>G</tt></span>GCCACCCAGG<span class="snp"><tt>-</tt></span>TGGCACTGGAGGCGCTCA 360


7033R-1.IR_full       361 CC<span class="snp"><tt>A</tt></span>CCGCCGCTCAGGACAACTCGTCCAGTGACGAGAAGTTCCGCAACGACCTGCTGAACA 420
                          ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     361 CC<span class="snp"><tt>G</tt></span>CCGCCGCTCAGGACAACTCGTCCAGTGACGAGAAGTTCCGCAACGACCTGCTGAACA 420


7033R-1.IR_full       421 TTGCCCGCACCACGCTCTCCTCGAAGATCCTGCACCAGCACAAAGACTTCTTCGCCAATC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7033R-1.in silico     421 TTGCCCGCACCACGCTCTCCTCGAAGATCCTGCACCAGCACAAAGACTTCTTCGCCAATC 480


7033R-1.IR_full       481 TGGCGGTGGATGCTGTGATGCGT 503
                          |||||||||||||||||||||||
7033R-1.in silico     481 TGGCGGTGGATGCTGTGATGCGT 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_176715.1	CG7033-RC, transcript variant C (CG7033), mRNA
100	482	0	0	3	NM_132296.2	CG7033-RA, transcript variant A (CG7033), mRNA
100	482	0	0	3	NM_167176.1	CG7033-RB, transcript variant B (CG7033), mRNA
0.2	1	3	3	4	NM_078544.2	CG1689-RA (lz), mRNA
0.2	1	2	2	3	NM_139861.2	CG8562-RA (CG8562), mRNA
0	0	13	23	12	NM_170016.1	CG5374-RB, transcript variant B (T-cp1), mRNA
0	0	13	23	12	NM_079726.3	CG5374-RA, transcript variant A (T-cp1), mRNA
0	0	5	18	12	NM_165867.1	CG8439-RB, transcript variant B (Cct5), mRNA
0	0	5	18	12	NM_078983.2	CG8439-RA, transcript variant A (Cct5), mRNA
0	0	5	3	6	NM_132235.2	CG32717-RB, transcript variant B (sdt), mRNA
0	0	5	3	6	NM_206653.1	CG32717-RE, transcript variant E (sdt), mRNA
0	0	5	3	6	NM_132236.3	CG32717-RA, transcript variant A (sdt), mRNA
0	0	3	3	2	NM_142147.2	CG7262-RA (CG7262), mRNA
0	0	2	8	11	NM_135735.1	CG5525-RA (CG5525), mRNA
0	0	2	4	18	NM_135247.2	CG9138-RA (SP1070), mRNA
0	0	2	3	14	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	2	3	14	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	2	3	14	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	2	3	14	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	2	3	4	NM_078862.2	CG17914-RA (yellow-b), mRNA
0	0	2	3	2	NM_135948.2	CG5809-RA (CaBP1), mRNA
0	0	2	2	3	NM_140342.1	CG17666-RA, transcript variant A (CG17666), mRNA
0	0	2	2	3	NM_168515.1	CG17666-RB, transcript variant B (CG17666), mRNA
0	0	1	9	7	NM_135812.2	CG16954-RA, transcript variant A (Hsp60D), mRNA
0	0	1	9	7	NM_165043.1	CG16954-RB, transcript variant B (Hsp60D), mRNA
0	0	1	5	23	NM_141578.1	CG8351-RA (CG8351), mRNA
0	0	1	5	2	NM_141983.2	CG7340-RB, transcript variant B (granny-smith), mRNA
0	0	1	5	2	NM_169483.1	CG7340-RC, transcript variant C (granny-smith), mRNA
0	0	1	5	2	NM_169482.1	CG7340-RA, transcript variant A (granny-smith), mRNA
0	0	1	4	9	NM_130545.2	CG11411-RA (fs(1)N), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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