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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6794R-1

Symbol Dif Full Name Dorsal-related immunity factor

CG No CG6794 Old CG No CG6794

Synonyms dif, DIF, CG6794, Dif

Accession No (Link to NCBI) NM_078865.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Nagata R, Nakamura M, Sanaki Y, Igaki T.
Cell Competition Is Driven by Autophagy.
Dev Cell (2019) 51(1) 99-112.e4 [ PubMed ID = 31543447 ] [ RRC reference ]

Yu S, Zhang G, Jin LH.
A high-sugar diet affects cellular and humoral immune responses in Drosophila.
Exp Cell Res (2018) 368(2) 215-224 [ PubMed ID = 29727694 ] [ RRC reference ]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCAGTGTGGCAGGAGCTGTT

Primer Seq. 3'

aatctagaggtaccGAGTCGCGCATCTCCAGCTTC

Predicted Fragment Size

IR fragment full Seq

0001 gGCAGNTGTG GCAGGAGCTG TTGGCGGANG cGGTGCTGCA CATCACATAT TATCGCAGTC 
0061 GACTTCCCTG CCGGTAATGC CGTCGCACAT TCCGCTCCAC CTGCAGAATC AGAATATGAA 
0121 TCAGAATCTG CCCGAGCCAA GTGCAAGAAG TGGTCCCCAC CTGCGTATCG TGGAGGAGCC 
0181 GACAAGCAAT ATAATCCGCT TTCGCTACAA ATGCGAGGGT CGCACCGCCG GTTCGATTCC 
0241 GGGCATGAAC TCCAGCTCGG AAACGGGCAA GACCTTTCCC ACCATCGAAG TGTGCAACTA 
0301 CGATGGACCC GTCATCATCG TGGTCTCCTG TGTGACGAGC GACGAGCCCT TNCCGCCAGC 
0361 ATCCCCACTG GCTGGTCAGC AAGGAGGAGG CGGATGCCTG CAAGTCGGGC ATCTACCAAA 
0421 AGAAATTGCC GCCAGAGGAG CGGCGGCTGG TGCTTCAAAA AGTGGGCATA CAGTGCGCCA 
0481 AGAAGCTGGA GATGCGCGAC TC

in silico PCR Fragment

0001 GGCAG-TGTG GCAGGAGCTG TTGGCGGAGG CGGTGCTGCA CATCACATAT TATCGCAGTC 
0061 GACTTCCCTG CCGGTAATGC CGTCGCACAT TCCGCTCCAC CTGCAGAATC AGAATATGAA 
0121 TCAGAATCTG CCCGAGCCAA GTGCAAGAAG TGGTCCCCAC CTGCGTATCG TGGAGGAGCC 
0181 GACAAGCAAT ATAATCCGCT TTCGCTACAA ATGCGAGGGT CGCACCGCCG GTTCGATTCC 
0241 GGGCATGAAC TCCAGCTCGG AAACGGGCAA GACCTTTCCC ACCATCGAAG TGTGCAACTA 
0301 CGATGGACCC GTCATCATCG TGGTCTCCTG TGTGACGAGC GACGAGCCCT T-CCGCCAGC 
0361 ATCCCCACTG GCTGGTCAGC AAGGAGGAGG CGGATGCCTG CAAGTCGGGC ATCTACCAAA 
0421 AGAAATTGCC GCCAGAGGAG CGGCGGCTGG TGCTCCAAAA AGTGGGCATA CAGTGCGCCA 
0481 AGAAGCTGGA GATGCGCGAC TC

Assemble Data


6794R-1.IR_full       1   GGCAGNTGTGGCAGGAGCTGTTGGCGGANGCGGTGCTGCACATCACATATTATCGCAGTC 60
                          ||||| |||||||||||||||||||||| |||||||||||||||||||||||||||||||
6794R-1.in silico     1   GGCAG-TGTGGCAGGAGCTGTTGGCGGAGGCGGTGCTGCACATCACATATTATCGCAGTC 60


6794R-1.IR_full       61  GACTTCCCTGCCGGTAATGCCGTCGCACATTCCGCTCCACCTGCAGAATCAGAATATGAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6794R-1.in silico     61  GACTTCCCTGCCGGTAATGCCGTCGCACATTCCGCTCCACCTGCAGAATCAGAATATGAA 120


6794R-1.IR_full       121 TCAGAATCTGCCCGAGCCAAGTGCAAGAAGTGGTCCCCACCTGCGTATCGTGGAGGAGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6794R-1.in silico     121 TCAGAATCTGCCCGAGCCAAGTGCAAGAAGTGGTCCCCACCTGCGTATCGTGGAGGAGCC 180


6794R-1.IR_full       181 GACAAGCAATATAATCCGCTTTCGCTACAAATGCGAGGGTCGCACCGCCGGTTCGATTCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6794R-1.in silico     181 GACAAGCAATATAATCCGCTTTCGCTACAAATGCGAGGGTCGCACCGCCGGTTCGATTCC 240


6794R-1.IR_full       241 GGGCATGAACTCCAGCTCGGAAACGGGCAAGACCTTTCCCACCATCGAAGTGTGCAACTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6794R-1.in silico     241 GGGCATGAACTCCAGCTCGGAAACGGGCAAGACCTTTCCCACCATCGAAGTGTGCAACTA 300


6794R-1.IR_full       301 CGATGGACCCGTCATCATCGTGGTCTCCTGTGTGACGAGCGACGAGCCCTTNCCGCCAGC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
6794R-1.in silico     301 CGATGGACCCGTCATCATCGTGGTCTCCTGTGTGACGAGCGACGAGCCCTT-CCGCCAGC 360


6794R-1.IR_full       361 ATCCCCACTGGCTGGTCAGCAAGGAGGAGGCGGATGCCTGCAAGTCGGGCATCTACCAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6794R-1.in silico     361 ATCCCCACTGGCTGGTCAGCAAGGAGGAGGCGGATGCCTGCAAGTCGGGCATCTACCAAA 420


6794R-1.IR_full       421 AGAAATTGCCGCCAGAGGAGCGGCGGCTGGTGCTTCAAAAAGTGGGCATACAGTGCGCCA 480
                          |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
6794R-1.in silico     421 AGAAATTGCCGCCAGAGGAGCGGCGGCTGGTGCTCCAAAAAGTGGGCATACAGTGCGCCA 480


6794R-1.IR_full       481 AGAAGCTGGAGATGCGCGACTC 502
                          ||||||||||||||||||||||
6794R-1.in silico     481 AGAAGCTGGAGATGCGCGACTC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_078865.2	CG6794-RA, transcript variant A (Dif), mRNA
100	482	0	0	2	NM_165216.1	CG6794-RB, transcript variant B (Dif), mRNA
0.62	3	4	7	8	NM_057746.3	CG11992-RA, transcript variant A (Rel), mRNA
0.62	3	4	7	8	NM_206467.1	CG11992-RB, transcript variant B (Rel), mRNA
0.62	3	4	7	8	NM_206466.1	CG11992-RC, transcript variant C (Rel), mRNA
0.62	3	4	7	8	NM_206465.1	CG11992-RD, transcript variant D (Rel), mRNA
0	0	4	2	4	NM_168156.1	CG7018-RA, transcript variant A (Ets65A), mRNA
0	0	4	2	4	NM_079221.2	CG7018-RB, transcript variant B (Ets65A), mRNA
0	0	3	4	2	NM_130478.2	CG2995-RA (CG2995), mRNA
0	0	2	4	6	NM_170304.1	CG31075-RA (CG31075), mRNA
0	0	2	4	6	NM_167538.1	CG32572-RA (CG32572), mRNA
0	0	2	3	9	NM_141009.2	CG32432-RA (CG32432), mRNA
0	0	2	2	3	NM_165698.1	CG1623-RA, transcript variant A (CG1623), mRNA
0	0	2	2	3	NM_136676.2	CG1623-RC, transcript variant C (CG1623), mRNA
0	0	2	2	3	NM_057325.2	CG3440-RA (Pcp), mRNA
0	0	2	2	3	NM_165700.1	CG1623-RD, transcript variant D (CG1623), mRNA
0	0	2	2	3	NM_165699.1	CG1623-RB, transcript variant B (CG1623), mRNA
0	0	2	2	3	NM_165701.1	CG1623-RE, transcript variant E (CG1623), mRNA
0	0	1	7	3	NM_136527.1	CG14755-RA (CG14755), mRNA
0	0	1	6	3	NM_143210.2	CG31323-RA (CG31323), mRNA
0	0	1	4	2	NM_170355.2	CG12877-RB, transcript variant B (CG12877), mRNA
0	0	1	4	2	NM_143327.2	CG12877-RA, transcript variant A (CG12877), mRNA
0	0	1	4	2	NM_001038984.1	CG12877-RC, transcript variant C (CG12877), mRNA
0	0	1	3	7	NM_141219.1	CG1078-RA (CG1078), mRNA
0	0	1	3	3	NM_078997.1	CG17584-RA (Or49b), mRNA
0	0	1	3	2	NM_135082.2	CG6604-RA (H15), mRNA
0	0	1	2	5	NM_133124.1	CG7502-RA (CG7502), mRNA
0	0	1	2	4	NM_136191.2	CG31688-RA (CG31688), mRNA
0	0	1	2	3	NM_140557.3	CG6114-RA (CG6114), mRNA
0	0	1	2	2	NM_132034.1	CG3108-RA (CG3108), mRNA

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