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Notice:
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Suspension of shipments during Golden Week Holidays: From 4 May to 11 May, deadline 21 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6716R-1 |
| Symbol |
prd |
Full Name |
paired |
| CG No |
CG6716 |
Old CG No |
CG6716 |
| Synonyms |
Prd, pr, CG6716, prd |
| Accession No (Link to NCBI) |
NM_078832.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAACGGTCGTCCTTTGCCCAAC |
| Primer Seq. 3' |
aatctagaggtaccCCCGGCTCACTTTCACAGTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AACGGTCGTC CTTTGCCCAA CAATATTCGT CTTAAAATCG TCGAGATGGC CGCCGATGGC
0061 ATTCGGCCCT GTGTGATCTC CAGACAGCTA CGTGTGTCCC ATGGCTGCGT ATCGAAGATC
0121 CTGAATCGCT ACCAGGAGAC TGGCTCCATT AGACCAGGCG TAATTGGTGG CTCCAAGCCG
0181 AGGATANGCC ACGCCCGAAA TCGAAAACCG AATTGAGGAG TACAAGCGCA GTAGCCCGGG
0241 CATGTTCTCG TGGGAGATCA GGGAGAAGCT GATCCGCGAG GGAGTCTGCG ACAGGAGCAC
0301 AGCACCATCT GTGTCCGCCA TATCGCGCCT GGTGCGCGGC CGNAGATGCT CCATTGGACA
0361 ATGATATGTC TTCTGCCTCT GGATCTCCGG CGGGTGATGG CACCAAAGCA TCGAGTTCCT
0421 GCGGCTCCGA TGTCTCAGGT GGCCATCACA ACAACGGCAA GCCCTCCGAT GAGGACATCT
0481 CTGACTGTGA AAGTGAGCCG GG |
| in silico PCR Fragment |
0001 AACGGTCGTC CTTTGCCCAA CAATATTCGT CTTAAAATCG TCGAGATGGC CGCCGATGGC
0061 ATTCGGCCCT GTGTGATCTC CAGACAGCTA CGTGTGTCCC ATGGCTGCGT ATCGAAGATC
0121 CTGAATCGCT ACCAGGAGAC TGGCTCCATT AGACCAGGCG TAATTGGTGG CTCCAAGCCG
0181 AGGATA-GCC ACGCCCGAAA TCGAGAACCG AATTGAGGAG TACAAGCGCA GTAGCCCGGG
0241 CATGTTCTCG TGGGAGATCA GGGAGAAGCT GATCCGCGAG GGTGTCTGCG ACAGGAGCAC
0301 AGCACCATCT GTGTCCGCCA TATCGCGCCT GGTGCGCGGC CG-AGATGCT CCATTGGACA
0361 ATGATATGTC TTCTGCCTCT GGATCTCCGG CGGGTGATGG CACCAAAGCA TCGAGTTCCT
0421 GCGGCTCCGA TGTCTCAGGT GGCCATCACA ACAACGGCAA GCCCTCCGAT GAGGACATCT
0481 CTGACTGTGA AAGTGAGCCG GG |
| Assemble Data |
6716R-1.IR_full 1 AACGGTCGTCCTTTGCCCAACAATATTCGTCTTAAAATCGTCGAGATGGCCGCCGATGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-1.in silico 1 AACGGTCGTCCTTTGCCCAACAATATTCGTCTTAAAATCGTCGAGATGGCCGCCGATGGC 60
6716R-1.IR_full 61 ATTCGGCCCTGTGTGATCTCCAGACAGCTACGTGTGTCCCATGGCTGCGTATCGAAGATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-1.in silico 61 ATTCGGCCCTGTGTGATCTCCAGACAGCTACGTGTGTCCCATGGCTGCGTATCGAAGATC 120
6716R-1.IR_full 121 CTGAATCGCTACCAGGAGACTGGCTCCATTAGACCAGGCGTAATTGGTGGCTCCAAGCCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-1.in silico 121 CTGAATCGCTACCAGGAGACTGGCTCCATTAGACCAGGCGTAATTGGTGGCTCCAAGCCG 180
6716R-1.IR_full 181 AGGATANGCCACGCCCGAAATCGAAAACCGAATTGAGGAGTACAAGCGCAGTAGCCCGGG 240
|||||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||
6716R-1.in silico 181 AGGATA-GCCACGCCCGAAATCGAGAACCGAATTGAGGAGTACAAGCGCAGTAGCCCGGG 240
6716R-1.IR_full 241 CATGTTCTCGTGGGAGATCAGGGAGAAGCTGATCCGCGAGGGAGTCTGCGACAGGAGCAC 300
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
6716R-1.in silico 241 CATGTTCTCGTGGGAGATCAGGGAGAAGCTGATCCGCGAGGGTGTCTGCGACAGGAGCAC 300
6716R-1.IR_full 301 AGCACCATCTGTGTCCGCCATATCGCGCCTGGTGCGCGGCCGNAGATGCTCCATTGGACA 360
|||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
6716R-1.in silico 301 AGCACCATCTGTGTCCGCCATATCGCGCCTGGTGCGCGGCCG-AGATGCTCCATTGGACA 360
6716R-1.IR_full 361 ATGATATGTCTTCTGCCTCTGGATCTCCGGCGGGTGATGGCACCAAAGCATCGAGTTCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-1.in silico 361 ATGATATGTCTTCTGCCTCTGGATCTCCGGCGGGTGATGGCACCAAAGCATCGAGTTCCT 420
6716R-1.IR_full 421 GCGGCTCCGATGTCTCAGGTGGCCATCACAACAACGGCAAGCCCTCCGATGAGGACATCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6716R-1.in silico 421 GCGGCTCCGATGTCTCAGGTGGCCATCACAACAACGGCAAGCCCTCCGATGAGGACATCT 480
6716R-1.IR_full 481 CTGACTGTGAAAGTGAGCCGGG 502
||||||||||||||||||||||
6716R-1.in silico 481 CTGACTGTGAAAGTGAGCCGGG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164990.2 |
CG6716-RB, transcript variant B (prd), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_078832.2 |
CG6716-RA, transcript variant A (prd), mRNA |
| 0.2 |
1 |
7 |
11 |
34 |
NM_079139.2 |
CG3388-RA (gsb), mRNA |
| 0 |
0 |
7 |
19 |
42 |
NM_079138.1 |
CG2692-RA (gsb-n), mRNA |
| 0 |
0 |
4 |
2 |
8 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0 |
0 |
3 |
6 |
16 |
NM_079317.2 |
CG10704-RA (toe), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_139759.1 |
CG6592-RA (CG6592), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_167943.1 |
CG32304-RA (CG32304), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_169609.1 |
CG31304-RA (CG31304), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132540.1 |
CG2467-RA, transcript variant A (CG2467), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_167313.1 |
CG2467-RB, transcript variant B (CG2467), mRNA |
| 0 |
0 |
1 |
9 |
8 |
NM_079318.2 |
CG10488-RA, transcript variant A (eyg), mRNA |
| 0 |
0 |
1 |
9 |
8 |
NM_001014582.1 |
CG10488-RB, transcript variant B (eyg), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_144457.2 |
CG18530-RA (CG18530), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_141602.2 |
CG11982-RA (CG11982), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139758.1 |
CG10475-RA (Jon65Ai), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079869.2 |
CG1528-RA, transcript variant A (gammaCop), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_170553.1 |
CG1528-RB, transcript variant B (gammaCop), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140321.1 |
CG10660-RA (CG10660), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001042910.1 |
CG11628-RB, transcript variant B (Grp1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079398.2 |
CG7930-RA, transcript variant A (TpnC73F), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168715.1 |
CG7930-RB, transcript variant B (TpnC73F), mRNA |
| 0 |
0 |
0 |
8 |
16 |
NM_057338.2 |
CG8246-RA (Poxn), mRNA |
| 0 |
0 |
0 |
7 |
6 |
NM_079808.2 |
CG5658-RA (Klp98A), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_167541.1 |
CG32569-RA (CG32569), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_141000.1 |
CG3698-RA (CG3698), mRNA |
| 0 |
0 |
0 |
4 |
20 |
NM_001043222.1 |
CG9610-RC (Poxm), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_143745.2 |
CG18389-RA (Eip93F), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_078869.4 |
CG15154-RB, transcript variant B (Socs36E), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165243.1 |
CG15154-RA, transcript variant A (Socs36E), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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