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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
6343R-2 |
| Symbol |
ND42 |
Full Name |
NADH:ubiquinone reductase 42kD subunit precursor |
| CG No |
CG6343 |
Old CG No |
CG6343 |
| Synonyms |
CG6343, ND42 |
| Accession No (Link to NCBI) |
NM_079721.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Chew LY, Zhang H, He J, Yu F.<br> The Nrf2-Keap1 pathway is activated by steroid hormone signaling to govern neuronal remodeling.<br> Cell Rep (2021) 36(5) 109466
[
PubMed ID = 34348164
]
[
RRC reference
]
Tan SWS, Yip GW, Suda T, Baeg GH.<br> Small Maf functions in the maintenance of germline stem cells in the Drosophila testis.<br> Redox Biol (2018) 15 125-134
[
PubMed ID = 29245136
]
[
RRC reference
]
Tan SWS, Lee QY, Wong BSE, Cai Y, Baeg GH.<br> Redox Homeostasis Plays Important Roles in the Maintenance of the Drosophila Testis Germline Stem Cells.<br> Stem Cell Reports (2017) 9(1) 342-354
[
PubMed ID = 28669604
]
[
RRC reference
]
Hochmuth CE, Biteau B, Bohmann D, Jasper H.<br> Redox regulation by Keap1 and Nrf2 controls intestinal stem cell proliferation in Drosophila.<br> Cell Stem Cell (2011) 8(2) 188-99
[
PubMed ID = 21295275
]
[
RRC reference
]
Owusu-Ansah E, Banerjee U.<br> Reactive oxygen species prime Drosophila haematopoietic progenitors for differentiation.<br> Nature (2009) 461(7263) 537-41
[
PubMed ID = 19727075
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCAGCATCTCCGGCAAGAC |
| Primer Seq. 3' |
aatctagaggtaccGCCGAAACATGGCCTCCATGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGCAGCATC TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG cCGCCCCCTA
0061 TCCGTACAAG ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG
0121 TTTTGACGAG AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA
0181 GTNTCGCCAA GGAACTGGCT GAGGAGCTGG ACATGGAGTA CTATCCGGCA GTTGATCTGG
0241 ACCTAATCTA CATCAATTCG TACGGCTATG ACATGAGGAA GTTGGATCCT CAGTTGCCGC
0301 CCAGCTGCCG AAGCTACGAT GTGCGCAACT TCTGCCTGGA TCCCAGCCAC GATCTGGCTG
0361 CCCAGTTCCA GATTCGCATG TACATgctgC GCNTATTCGC AGTACATTGA TgNCCTGCAG
0421 CACGTCCTTA GCACCGGCCA GGGTGTTGTC CTCGAgNGCA GCCCCTACTC GGACTTTGTC
0481 TTCATGGAGG CCATGTTTCG GC |
| in silico PCR Fragment |
0001 ATGCAGCATC TCCGGCAAGA CGATGCGAGG TGGACCACGG GTGCCCAAGG CCGCCCCCTA
0061 TCCGTACAAG ACGAAGAAGT ACAGCGTGTT CAATGCTATT TTCGACAAGA CCTCCAAGCG
0121 TTTTGACGAG AACTCCAAGG TGATCTGTGT GGAGGGACCC ATCGCGGCCG GCAAGTCAAA
0181 GT-TCGCCAA GGAACTGGCT GAGGAGCTGG ACATGGAGTA CTATCCGGCA GTTGATCTGG
0241 ACCTAATCTA CATCAATTCG TACGGCTATG ACATGAGGAA GTTGGATCCT CAGTTGCCGC
0301 CCAGCTGCCG AAGCTACGAT GTGCGCAACT TCTGCCTGGA TCCCAGCCAC GATCTGGCTG
0361 CCCAGTTCCA GATTCGCATG TACATGCTGC GC-TATTCGC AGTACATTGA TGCCCTGCAG
0421 CACGTCCTTA GCACCGGCCA GGGTGTTGTC CTCGAGCGCA GCCCCTACTC GGACTTTGTC
0481 TTCATGGAGG CCATGTTTCG GC |
| Assemble Data |
<PRE>
6343R-2.IR_full 1 ATGCAGCATCTCCGGCAAGACGATGCGAGGTGGACCACGGGTGCCCAAGGCCGCCCCCTA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 1 ATGCAGCATCTCCGGCAAGACGATGCGAGGTGGACCACGGGTGCCCAAGGCCGCCCCCTA 60
6343R-2.IR_full 61 TCCGTACAAGACGAAGAAGTACAGCGTGTTCAATGCTATTTTCGACAAGACCTCCAAGCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 61 TCCGTACAAGACGAAGAAGTACAGCGTGTTCAATGCTATTTTCGACAAGACCTCCAAGCG 120
6343R-2.IR_full 121 TTTTGACGAGAACTCCAAGGTGATCTGTGTGGAGGGACCCATCGCGGCCGGCAAGTCAAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 121 TTTTGACGAGAACTCCAAGGTGATCTGTGTGGAGGGACCCATCGCGGCCGGCAAGTCAAA 180
6343R-2.IR_full 181 GT<span class="snp"><tt>N</tt></span>TCGCCAAGGAACTGGCTGAGGAGCTGGACATGGAGTACTATCCGGCAGTTGATCTGG 240
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 181 GT<span class="snp"><tt>-</tt></span>TCGCCAAGGAACTGGCTGAGGAGCTGGACATGGAGTACTATCCGGCAGTTGATCTGG 240
6343R-2.IR_full 241 ACCTAATCTACATCAATTCGTACGGCTATGACATGAGGAAGTTGGATCCTCAGTTGCCGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 241 ACCTAATCTACATCAATTCGTACGGCTATGACATGAGGAAGTTGGATCCTCAGTTGCCGC 300
6343R-2.IR_full 301 CCAGCTGCCGAAGCTACGATGTGCGCAACTTCTGCCTGGATCCCAGCCACGATCTGGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6343R-2.in silico 301 CCAGCTGCCGAAGCTACGATGTGCGCAACTTCTGCCTGGATCCCAGCCACGATCTGGCTG 360
6343R-2.IR_full 361 CCCAGTTCCAGATTCGCATGTACATGCTGCGC<span class="snp"><tt>N</tt></span>TATTCGCAGTACATTGATG<span class="snp"><tt>N</tt></span>CCTGCAG 420
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||
6343R-2.in silico 361 CCCAGTTCCAGATTCGCATGTACATGCTGCGC<span class="snp"><tt>-</tt></span>TATTCGCAGTACATTGATG<span class="snp"><tt>C</tt></span>CCTGCAG 420
6343R-2.IR_full 421 CACGTCCTTAGCACCGGCCAGGGTGTTGTCCTCGAG<span class="snp"><tt>N</tt></span>GCAGCCCCTACTCGGACTTTGTC 480
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
6343R-2.in silico 421 CACGTCCTTAGCACCGGCCAGGGTGTTGTCCTCGAG<span class="snp"><tt>C</tt></span>GCAGCCCCTACTCGGACTTTGTC 480
6343R-2.IR_full 481 TTCATGGAGGCCATGTTTCGGC 502
||||||||||||||||||||||
6343R-2.in silico 481 TTCATGGAGGCCATGTTTCGGC 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_079721.2 |
CG6343-RA, transcript variant A (ND42), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_169990.1 |
CG6343-RB, transcript variant B (ND42), mRNA |
| 0.41 |
2 |
2 |
2 |
2 |
NM_136855.1 |
CG13193-RA (CG13193), mRNA |
| 0 |
0 |
7 |
2 |
5 |
NM_142054.1 |
CG14365-RA (CG14365), mRNA |
| 0 |
0 |
2 |
7 |
4 |
NM_142642.1 |
CG4159-RA (CG4159), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057771.2 |
CG1864-RB, transcript variant B (Hr38), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_176065.1 |
CG1864-RC, transcript variant C (Hr38), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164725.1 |
CG9188-RE, transcript variant E (sip2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164724.1 |
CG9188-RD, transcript variant D (sip2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135246.2 |
CG9188-RA, transcript variant A (sip2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164722.1 |
CG9188-RB, transcript variant B (sip2), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_164723.1 |
CG9188-RC, transcript variant C (sip2), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_142562.1 |
CG6231-RA, transcript variant A (CG6231), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_169864.1 |
CG6231-RB, transcript variant B (CG6231), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_169866.1 |
CG6231-RD, transcript variant D (CG6231), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_169865.1 |
CG6231-RC, transcript variant C (CG6231), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_001042796.1 |
CG6775-RC, transcript variant C (rg), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_080023.1 |
CG6775-RA, transcript variant A (rg), mRNA |
| 0 |
0 |
1 |
4 |
11 |
NM_167028.1 |
CG6775-RB, transcript variant B (rg), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_176746.1 |
CG5424-RC, transcript variant C (f), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_176745.1 |
CG5424-RD, transcript variant D (f), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_001042819.1 |
CG5424-RF, transcript variant F (f), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_078660.2 |
CG5424-RB, transcript variant B (f), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057604.3 |
CG1825-RA (Map60), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142893.1 |
CG10177-RA (CG10177), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_133073.2 |
CG6394-RA, transcript variant A (GalNAc-T2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167623.1 |
CG6394-RB, transcript variant B (GalNAc-T2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130672.2 |
CG2712-RA (CG2712), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140817.1 |
CG14076-RA (CG14076), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_135101.1 |
CG18266-RA (CG18266), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| +- |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00190
:
Oxidative phosphorylation
dme01100
:
Metabolic pathways
|
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|
