|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5988R-3 |
| Symbol |
upd2 |
Full Name |
unpaired 2 |
| CG No |
CG5988 |
Old CG No |
CG5988 |
| Synonyms |
Upd2, CG5988, cg5988, upd2, UPD2 |
| Accession No (Link to NCBI) |
NM_133049.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Liu Z, Zhang H, Lemaitre B, Li X.<br> Duox activation in Drosophila Malpighian tubules stimulates intestinal epithelial renewal through a countercurrent flow.<br> Cell Rep (2024) 43(4) 114109
[
PubMed ID = 38613782
]
[
RRC reference
]
Juarez-Carreño S, Vallejo DM, Carranza-Valencia J, Palomino-Schätzlein M, Ramon-Cañellas P, Santoro R, de Hartog E, Ferres-Marco D, Romero A, Peterson HP, Ballesta-Illan E, Pineda-Lucena A, Dominguez M, Morante J.<br> Body-fat sensor triggers ribosome maturation in the steroidogenic gland to initiate sexual maturation in Drosophila.<br> Cell Rep (2021) 37(2) 109830
[
PubMed ID = 34644570
]
[
RRC reference
]
Chakrabarti S, Visweswariah SS.<br> Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation.<br> Cell Rep (2020) 33(6) 108368
[
PubMed ID = 33176146
]
[
RRC reference
]
Iatsenko I, Boquete JP, Lemaitre B.<br> Microbiota-Derived Lactate Activates Production of Reactive Oxygen Species by the Intestinal NADPH Oxidase Nox and Shortens Drosophila Lifespan.<br> Immunity (2018) 49(5) 929-942.e5
[
PubMed ID = 30446385
]
[
RRC reference
]
Lee JH, Lee CW, Park SH, Choe KM.<br> Spatiotemporal regulation of cell fusion by JNK and JAK/STAT signaling during <i>Drosophila</i> wound healing.<br> J Cell Sci (2017) 130(11) 1917-1928
[
PubMed ID = 28424232
]
[
RRC reference
]
Zhai Z, Kondo S, Ha N, Boquete JP, Brunner M, Ueda R, Lemaitre B.<br> Accumulation of differentiating intestinal stem cell progenies drives tumorigenesis.<br> Nat Commun (2015) 6 10219
[
PubMed ID = 26690827
]
[
RRC reference
]
Patel PH, Dutta D, Edgar BA.<br> Niche appropriation by Drosophila intestinal stem cell tumours.<br> Nat Cell Biol (2015) 17(9) 1182-92
[
PubMed ID = 26237646
]
[
RRC reference
]
Rajan A, Perrimon N.<br> Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion.<br> Cell (2012) 151(1) 123-37
[
PubMed ID = 23021220
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAGCCAGCCAACAGAGCCAA |
| Primer Seq. 3' |
aatctagaggtaccCTGCTATCGCTGAGGCTCTCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAGCCAGCC AACAGAGCCA AAGCAGCCGC AGCAGCAGGA GTGCACACAG CAGCTGGAGC
0061 TGCCACAGCC GGCAGGTGCT GCTAGTGATC ATGATCCTGA GCGTCGTGAT GCCATTCACC
0121 AAGGCGCGAC ACTTGCGCGA CCAGGTGGCA CTGGACTACG CCTACGACGA GGACAACTCC
0181 GGCCGATCTT CCTCGTCGTC ATCGTCATCC TCATCATCGG GTGGCGACAT CCTGCCCGCC
0241 TTCGATGTCA ATCCGTATCG CGGTCTGGCC GATAGCTTCG GCGAGGGCAG CTACGACAGT
0301 GATATGAGTC TCAGCGAGAG TAGCTACGAG GAGATCGCCC AGGCGGCCAT CCGGGCTGCC
0361 AGGCGGNGAG ATGCGCCGTC AGAGGACGCG ACACNGCGcG CAGCCACAAC CTGCGACTCT
0421 TCTCCGGCAA ATCAGAGATC CCGGAGTGGG AGAACCCGTG CGGAGGCACC TACACGCCCG
0481 ACGAGAGCCT CAGCGATAAG GGGCAG |
| in silico PCR Fragment |
0001 GCAGCCAGCC AACAGAGCCA AAGCAGCCGC AGCAGCAGGA GTGCACACAG CAGCTGGAGC
0061 TGCCACAGCC GGCAGGTGCT GCTAGTGATC ATGATCCTGA GCGTCGTGAT GCCATTCACC
0121 AAGGCGCGAC ACTTGCGCGA CCAGGTGGCA CTGGACTACG CCTACGACGA GGACAACTCC
0181 GGCCGATCTT CCTCGTCGTC ATCGTCATCC TCATCATCGG GTGGCGACAT CCTGCCCGCC
0241 TTCGATGTCA ATCCGTATCG CGGTCTGGCC GATAGCTTCG GCGAGGGCAG CTACGACAGT
0301 GATATGAGTC TCAGCGAGAG TAGCTACGAG GAGATCGCCC AGGCGGCCAT CCGGGCTGCC
0361 AGGCGG-GAG ATGCGCCGTC AGAGGACGCG ACAC-GCGCG CAGCCACAAC CTGCGACTCT
0421 TCTCCGGCAA ATCAGAGATC CCGGAGTGGG AGAACCCGTG CGGAGGCACC TACACGCCCG
0481 ACGAGAGCCT CAGCGATA-- --GCAG |
| Assemble Data |
<PRE>
5988R-3.IR_full 1 GCAGCCAGCCAACAGAGCCAAAGCAGCCGCAGCAGCAGGAGTGCACACAGCAGCTGGAGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 1 GCAGCCAGCCAACAGAGCCAAAGCAGCCGCAGCAGCAGGAGTGCACACAGCAGCTGGAGC 60
5988R-3.IR_full 61 TGCCACAGCCGGCAGGTGCTGCTAGTGATCATGATCCTGAGCGTCGTGATGCCATTCACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 61 TGCCACAGCCGGCAGGTGCTGCTAGTGATCATGATCCTGAGCGTCGTGATGCCATTCACC 120
5988R-3.IR_full 121 AAGGCGCGACACTTGCGCGACCAGGTGGCACTGGACTACGCCTACGACGAGGACAACTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 121 AAGGCGCGACACTTGCGCGACCAGGTGGCACTGGACTACGCCTACGACGAGGACAACTCC 180
5988R-3.IR_full 181 GGCCGATCTTCCTCGTCGTCATCGTCATCCTCATCATCGGGTGGCGACATCCTGCCCGCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 181 GGCCGATCTTCCTCGTCGTCATCGTCATCCTCATCATCGGGTGGCGACATCCTGCCCGCC 240
5988R-3.IR_full 241 TTCGATGTCAATCCGTATCGCGGTCTGGCCGATAGCTTCGGCGAGGGCAGCTACGACAGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 241 TTCGATGTCAATCCGTATCGCGGTCTGGCCGATAGCTTCGGCGAGGGCAGCTACGACAGT 300
5988R-3.IR_full 301 GATATGAGTCTCAGCGAGAGTAGCTACGAGGAGATCGCCCAGGCGGCCATCCGGGCTGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 301 GATATGAGTCTCAGCGAGAGTAGCTACGAGGAGATCGCCCAGGCGGCCATCCGGGCTGCC 360
5988R-3.IR_full 361 AGGCGG<span class="snp"><tt>N</tt></span>GAGATGCGCCGTCAGAGGACGCGACAC<span class="snp"><tt>N</tt></span>GCGCGCAGCCACAACCTGCGACTCT 420
||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||
5988R-3.in silico 361 AGGCGG<span class="snp"><tt>-</tt></span>GAGATGCGCCGTCAGAGGACGCGACAC<span class="snp"><tt>-</tt></span>GCGCGCAGCCACAACCTGCGACTCT 420
5988R-3.IR_full 421 TCTCCGGCAAATCAGAGATCCCGGAGTGGGAGAACCCGTGCGGAGGCACCTACACGCCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5988R-3.in silico 421 TCTCCGGCAAATCAGAGATCCCGGAGTGGGAGAACCCGTGCGGAGGCACCTACACGCCCG 480
5988R-3.IR_full 481 ACGAGAGCCTCAGCGATA<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span>GCAG 506
||||||||||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||
5988R-3.in silico 481 ACGAGAGCCTCAGCGATA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GCAG 506
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
11 |
NM_133049.1 |
CG5988-RA (upd2), mRNA |
| 1.03 |
5 |
4 |
5 |
8 |
NM_136862.1 |
CG8271-RA (CG8271), mRNA |
| 0.82 |
4 |
3 |
2 |
19 |
NM_139371.1 |
CG13917-RA (CG13917), mRNA |
| 0.41 |
2 |
6 |
4 |
20 |
NM_135786.1 |
CG6108-RA (CG6108), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_167634.1 |
CG7088-RB, transcript variant B (bnb), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_078682.2 |
CG7088-RC, transcript variant C (bnb), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_167633.1 |
CG7088-RA, transcript variant A (bnb), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_167635.1 |
CG7088-RD, transcript variant D (bnb), mRNA |
| 0 |
0 |
11 |
12 |
13 |
NM_135892.2 |
CG4185-RA (NC2beta), mRNA |
| 0 |
0 |
9 |
36 |
128 |
NM_168401.1 |
CG6611-RC, transcript variant C (ect), mRNA |
| 0 |
0 |
9 |
36 |
118 |
NM_168400.1 |
CG6611-RB, transcript variant B (ect), mRNA |
| 0 |
0 |
9 |
36 |
118 |
NM_079967.4 |
CG6611-RA, transcript variant A (ect), mRNA |
| 0 |
0 |
9 |
24 |
89 |
NM_135392.3 |
CG13096-RA (CG13096), mRNA |
| 0 |
0 |
9 |
14 |
19 |
NM_132877.1 |
CG8958-RA (CG8958), mRNA |
| 0 |
0 |
7 |
2 |
12 |
NM_165024.1 |
CG31856-RA (CG31856), mRNA |
| 0 |
0 |
6 |
26 |
69 |
NM_167521.2 |
CG32575-RA, transcript variant A (hang), mRNA |
| 0 |
0 |
6 |
24 |
53 |
NM_167387.1 |
CG32611-RB (CG32611), mRNA |
| 0 |
0 |
6 |
22 |
57 |
NM_167520.2 |
CG32575-RB, transcript variant B (hang), mRNA |
| 0 |
0 |
6 |
20 |
96 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 0 |
0 |
6 |
20 |
89 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 0 |
0 |
6 |
8 |
30 |
NM_166314.1 |
CG12758-RA, transcript variant A (sano), mRNA |
| 0 |
0 |
6 |
8 |
30 |
NM_166315.1 |
CG12758-RB, transcript variant B (sano), mRNA |
| 0 |
0 |
6 |
8 |
20 |
NM_079064.2 |
CG12758-RD, transcript variant D (sano), mRNA |
| 0 |
0 |
6 |
8 |
20 |
NM_166316.1 |
CG12758-RC, transcript variant C (sano), mRNA |
| 0 |
0 |
6 |
6 |
10 |
NM_134748.2 |
CG5423-RA (robo3), mRNA |
| 0 |
0 |
5 |
37 |
148 |
NM_131975.3 |
CG32767-RA, transcript variant A (CG32767), mRNA |
| 0 |
0 |
5 |
37 |
148 |
NM_206628.1 |
CG32767-RB, transcript variant B (CG32767), mRNA |
| 0 |
0 |
5 |
7 |
22 |
NM_057617.3 |
CG10497-RA, transcript variant A (Sdc), mRNA |
| 0 |
0 |
5 |
6 |
39 |
NM_136553.2 |
CG8639-RA (Cirl), mRNA |
| 0 |
0 |
5 |
5 |
8 |
NM_142698.1 |
CG3353-RA (CG3353), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04391
:
Hippo signaling pathway - fly
|
|
|
