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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5933R-4 |
| Symbol |
CG5933 |
Full Name |
CG5933 |
| CG No |
CG5933 |
Old CG No |
CG5933 |
| Synonyms |
CG5933 |
| Accession No (Link to NCBI) |
NM_142947.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal, female semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kao SY, Nikonova E, Chaabane S, Sabani A, Martitz A, Wittner A, Heemken J, Straub T, Spletter ML.<br> A Candidate RNAi Screen Reveals Diverse RNA-Binding Protein Phenotypes in <i>Drosophila</i> Flight Muscle.<br> Cells (2021) 10(10)
[
PubMed ID = 34685485
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGAAGCTGGAAAAGCGCAAG |
| Primer Seq. 3' |
aatctagaggtaccGGCATGGATAGCAGCATCATA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAGAAGCTGG AAAAGCGCAA GAAGGAGCGC ATTGAGATTC TCTCGGACAT TCAGGAGGAT
0061 CTGACGAATC CCAAAAAGGA ACTCGTTGAG GCTGACTTGG AAGTACAGAA GGAGGTGCTG
0121 CAGGCCCTCA GTTCCTGCTC CCTGGCCCTG NCCATTGTCT NCCACACAGG TGGTGGAAAA
0181 GATTGCCGGC AGCAGCCTGG AGATGGTGAA CTTTATTCTA GGCAAACTGG CCAACCAGGG
0241 TGCAATTGTG ATCCGGAACG TGACCATCGG CACCGAGGCC GGCTGCGAAA TCATCTCTGT
0301 GCAGCCCAAG GAGCTGAAGG AGATCCTGGA AGACACCAAC GATACGTGTC AGCAAAAGGA
0361 GGAGGAGGCC AAGAGAAAGT TGGAAGTCGA CGATGTTGAT CAGCCACAGG AGAAGACAAT
0421 AAAACTGGAG TCCACTGTAG CGCGGAAAGA GTCCACAAGC CTGGATGCTC CGGACGATAT
0481 TATGATGCTG CTATCCATGC C |
| in silico PCR Fragment |
0001 GAGAAGCTGG AAAAGCGCAA GAAGGAGCGC ATTGAGATTC TCTCGGACAT TCAGGAGGAT
0061 CTGACGAATC CCAAAAAGGA ACTCGTTGAG GCTGACTTGG AAGTACAGAA GGAGGTGCTG
0121 CAGGCCCTCA GTTCCTGCTC CCTGGCCCTG CCCATTGTCT -CCACACAGG TGGTGGAAAA
0181 GATTGCCGGC AGCAGCCTGG AGATGGTGAA CTTTATTCTA GGCAAACTGG CCAACCAGGG
0241 TGCAATTGTG ATCCGGAACG TGACCATCGG CACCGAGGCC GGCTGCGAAA TCATCTCTGT
0301 GCAGCCCAAG GAGCTGAAGG AGATCCTGGA AGACACCAAC GATACGTGTC AGCAAAAGGA
0361 GGAGGAGGCC AAGAGAAAGT TGGAAGTCGA CGATGTTGAT CAGCCACAGG AGAAGACAAT
0421 AAAACTGGAG TCCACTGTAG CGCGGAAAGA GTCCACAAGC CTGGATGCTC CGGACGATAT
0481 TATGATGCTG CTATCCATGC C |
| Assemble Data |
<PRE>
5933R-4.IR_full 1 AGAAGCTGGAAAAGCGCAAGAAGGAGCGCATTGAGATTCTCTCGGACATTCAGGAGGAT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 1 AGAAGCTGGAAAAGCGCAAGAAGGAGCGCATTGAGATTCTCTCGGACATTCAGGAGGAT 59
5933R-4.IR_full 61 CTGACGAATCCCAAAAAGGAACTCGTTGAGGCTGACTTGGAAGTACAGAAGGAGGTGCTG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 61 CTGACGAATCCCAAAAAGGAACTCGTTGAGGCTGACTTGGAAGTACAGAAGGAGGTGCTG 119
5933R-4.IR_full 121 CAGGCCCTCAGTTCCTGCTCCCTGGCCCTG<span class="snp"><tt>N</tt></span>CCATTGTCT<span class="snp"><tt>N</tt></span>CCACACAGGTGGTGGAAAA 179
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
5933R-4.in silico 121 CAGGCCCTCAGTTCCTGCTCCCTGGCCCTG<span class="snp"><tt>C</tt></span>CCATTGTCT<span class="snp"><tt>-</tt></span>CCACACAGGTGGTGGAAAA 179
5933R-4.IR_full 181 GATTGCCGGCAGCAGCCTGGAGATGGTGAACTTTATTCTAGGCAAACTGGCCAACCAGGG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 181 GATTGCCGGCAGCAGCCTGGAGATGGTGAACTTTATTCTAGGCAAACTGGCCAACCAGGG 239
5933R-4.IR_full 241 TGCAATTGTGATCCGGAACGTGACCATCGGCACCGAGGCCGGCTGCGAAATCATCTCTGT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 241 TGCAATTGTGATCCGGAACGTGACCATCGGCACCGAGGCCGGCTGCGAAATCATCTCTGT 299
5933R-4.IR_full 301 GCAGCCCAAGGAGCTGAAGGAGATCCTGGAAGACACCAACGATACGTGTCAGCAAAAGGA 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 301 GCAGCCCAAGGAGCTGAAGGAGATCCTGGAAGACACCAACGATACGTGTCAGCAAAAGGA 359
5933R-4.IR_full 361 GGAGGAGGCCAAGAGAAAGTTGGAAGTCGACGATGTTGATCAGCCACAGGAGAAGACAAT 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 361 GGAGGAGGCCAAGAGAAAGTTGGAAGTCGACGATGTTGATCAGCCACAGGAGAAGACAAT 419
5933R-4.IR_full 421 AAAACTGGAGTCCACTGTAGCGCGGAAAGAGTCCACAAGCCTGGATGCTCCGGACGATAT 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5933R-4.in silico 421 AAAACTGGAGTCCACTGTAGCGCGGAAAGAGTCCACAAGCCTGGATGCTCCGGACGATAT 479
5933R-4.IR_full 481 TATGATGCTGCTATCCATGCC 500
|||||||||||||||||||||
5933R-4.in silico 481 TATGATGCTGCTATCCATGCC 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_142947.2 |
CG5933-RA (CG5933), mRNA |
| 0.2 |
1 |
5 |
2 |
4 |
NM_164411.2 |
CG4896-RB, transcript variant B (CG4896), mRNA |
| 0.2 |
1 |
5 |
2 |
4 |
NM_134739.5 |
CG4896-RD, transcript variant D (CG4896), mRNA |
| 0.2 |
1 |
5 |
2 |
4 |
NM_164410.2 |
CG4896-RA, transcript variant A (CG4896), mRNA |
| 0.2 |
1 |
5 |
2 |
4 |
NM_164409.2 |
CG4896-RC, transcript variant C (CG4896), mRNA |
| 0 |
0 |
6 |
5 |
4 |
NM_132275.1 |
CG1994-RA (l(1)G0020), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_166566.1 |
CG3504-RA (inaD), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_131924.2 |
CG4857-RB (CG4857), mRNA |
| 0 |
0 |
3 |
3 |
7 |
NM_135976.2 |
CG31743-RA (CG31743), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001042853.1 |
CG12473-RA, transcript variant A (stnB), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001042852.1 |
CG12500-RB, transcript variant B (stnA), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001042851.1 |
CG12500-RA, transcript variant A (stnA), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001042854.1 |
CG12473-RB, transcript variant B (stnB), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_175944.1 |
CG4280-RB, transcript variant B (crq), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_175943.1 |
CG4280-RA, transcript variant A (crq), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_169061.1 |
CG4280-RA, transcript variant A (crq), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_169060.1 |
CG4280-RA, transcript variant A (crq), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_080498.2 |
CG5942-RA, transcript variant A (brm), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_168640.1 |
CG5942-RD, transcript variant D (brm), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_080497.4 |
CG5942-RC, transcript variant C (brm), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_168641.1 |
CG5942-RB, transcript variant B (brm), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_137390.2 |
CG6484-RA (CG6484), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_133002.1 |
CG5800-RA (CG5800), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_164565.1 |
CG3399-RC, transcript variant C (capu), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_057618.2 |
CG3399-RA, transcript variant A (capu), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_164567.1 |
CG3399-RB, transcript variant B (capu), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_164566.1 |
CG3399-RD, transcript variant D (capu), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057751.2 |
CG8161-RA (Rlb1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167309.1 |
CG32663-RA (CG32663), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_136690.2 |
CG1513-RA (CG1513), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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