|
|
| NIG-RNAi Strains Detail |
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5680R-1 |
| Symbol |
bsk |
Full Name |
basket |
| CG No |
CG5680 |
Old CG No |
CG5680 |
| Synonyms |
JNK, DJNK, jnk, dJNK, DBSK/JNK, D-JNK, CG5680, DJNK/bsk, JNK/SAPK, SAPKa, D-junk, Bsk, Junk, bsk |
| Accession No (Link to NCBI) |
NM_164900.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fairchild MJ, Islam F, Tanentzapf G.<br> Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.<br> PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Tsai CR, Anderson AE, Burra S, Jo J, Galko MJ.<br> Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.<br> Dev Biol (2017) 427(1) 61-71
[
PubMed ID = 28514643
]
[
RRC reference
]
Lee JH, Lee CW, Park SH, Choe KM.<br> Spatiotemporal regulation of cell fusion by JNK and JAK/STAT signaling during <i>Drosophila</i> wound healing.<br> J Cell Sci (2017) 130(11) 1917-1928
[
PubMed ID = 28424232
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Wang Y, Antunes M, Anderson AE, Kadrmas JL, Jacinto A, Galko MJ.<br> Integrin Adhesions Suppress Syncytium Formation in the Drosophila Larval Epidermis.<br> Curr Biol (2015) 25(17) 2215-27
[
PubMed ID = 26255846
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Brock AR, Wang Y, Berger S, Renkawitz-Pohl R, Han VC, Wu Y, Galko MJ.<br> Transcriptional regulation of Profilin during wound closure in Drosophila larvae.<br> J Cell Sci (2012) 125(Pt 23) 5667-76
[
PubMed ID = 22976306
]
[
RRC reference
]
Ahn HM, Lee KS, Lee DS, Yu K.<br> JNK/FOXO mediated PeroxiredoxinV expression regulates redox homeostasis during Drosophila melanogaster gut infection.<br> Dev Comp Immunol (2012) 38(3) 466-73
[
PubMed ID = 22858408
]
[
RRC reference
]
Gonda RL, Garlena RA, Stronach B.<br> Drosophila heat shock response requires the JNK pathway and phosphorylation of mixed lineage kinase at a conserved serine-proline motif.<br> PLoS One (2012) 7(7) e42369
[
PubMed ID = 22848763
]
[
RRC reference
]
Bangi E, Pitsouli C, Rahme LG, Cagan R, Apidianakis Y.<br> Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells.<br> EMBO Rep (2012) 13(6) 569-76
[
PubMed ID = 22498775
]
[
RRC reference
]
Tsuzuki S, Ochiai M, Matsumoto H, Kurata S, Ohnishi A, Hayakawa Y.<br> Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress.<br> Sci Rep (2012) 2 210
[
PubMed ID = 22355724
]
[
RRC reference
]
Doggett K, Grusche FA, Richardson HE, Brumby AM.<br> Loss of the Drosophila cell polarity regulator Scribbled promotes epithelial tissue overgrowth and cooperation with oncogenic Ras-Raf through impaired Hippo pathway signaling.<br> BMC Dev Biol (2011) 11 57
[
PubMed ID = 21955824
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.<br> A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.<br> Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Neisch AL, Speck O, Stronach B, Fehon RG.<br> Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.<br> J Cell Biol (2010) 189(2) 311-23
[
PubMed ID = 20404112
]
[
RRC reference
]
Zhu M, Xin T, Weng S, Gao Y, Zhang Y, Li Q, Li M.<br> Activation of JNK signaling links lgl mutations to disruption of the cell polarity and epithelial organization in Drosophila imaginal discs.<br> Cell Res (2010) 20(2) 242-5
[
PubMed ID = 20066009
]
[
RRC reference
]
Wu Y, Brock AR, Wang Y, Fujitani K, Ueda R, Galko MJ.<br> A blood-borne PDGF/VEGF-like ligand initiates wound-induced epidermal cell migration in Drosophila larvae.<br> Curr Biol (2009) 19(17) 1473-7
[
PubMed ID = 19646875
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.<br> Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.<br> Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H.<br> PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis.<br> EMBO J (2004) 23(20) 3984-94
[
PubMed ID = 15457211
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACCAACACTACACCGTCGAG |
| Primer Seq. 3' |
aatctagaggtaccCGTGCCAGACCGAAATCCAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACCAACACT ACACCGTCGA GGTgGGGGAC ACCAACTTCA CCATCCACAG TCGGTACATT
0061 AATCTACGGC CCATAGGATC AGGTGCCCAA GGAATAGTAT GCGCCGCTTA CGATACTATC
0121 ACCCAGCAAA ATGTGGCGAT TAAGAAACTA TCTCGACCAT TCCAAAATGT AACCCATGCC
0181 AAGCGAGCAT ATAGGGAATT CAAGCTAATG AAGCTTGTTA ACCATAAAAA CATAATTGGC
0241 TTACTTAATG CTTTTACGCC GCAAAGGAAC TTGGAAGAGT TTCAGGATGT CTACCTGGTC
0301 ATGGAGCTGA TGGACGCTAA TCTCTGCCAG GTCATCCAAA TGGACTTGGA TCACGACAGA
0361 ATGTCCTATT TGCTTTATCA AATGTTGTGC GGAATAAAAC ATTTACACTC AGCAGGAATT
0421 ATTCACAGAG ACTTAAAGCC ATCCAATATA GTTGTAAAGG CCGACTGCAC TCTAAAAATT
0481 TTGGATTTCG GTCTGGCACG |
| in silico PCR Fragment |
0001 CACCAACACT ACACCGTCGA GGTGGGGGAC ACCAACTTCA CCATCCACAG TCGGTACATT
0061 AATCTACGGC CCATAGGATC AGGTGCCCAA GGAATAGTAT GCGCCGCTTA CGATACTATC
0121 ACCCAGCAAA ATGTGGCGAT TAAGAAACTA TCTCGACCAT TCCAAAATGT AACCCATGCC
0181 AAGCGAGCAT ATAGGGAATT CAAGCTAATG AAGCTTGTTA ACCATAAAAA CATAATTGGC
0241 TTACTTAATG CTTTTACGCC GCAAAGGAAC TTGGAAGAGT TTCAGGATGT CTACCTGGTC
0301 ATGGAGCTGA TGGACGCTAA TCTCTGCCAG GTCATCCAAA TGGACTTGGA TCACGACAGA
0361 ATGTCCTATT TGCTTTATCA AATGTTGTGC GGAATAAAAC ATTTACACTC AGCAGGAATT
0421 ATTCACAGAG ACTTAAAGCC ATCCAATATA GTTGTAAAGG CCGACTGCAC TCTAAAAATT
0481 TTGGATTTCG GTCTGGCACG |
| Assemble Data |
<PRE>
5680R-1.IR_full 1 CACCAACACTACACCGTCGAGGTGGGGGACACCAACTTCACCATCCACAGTCGGTACATT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 1 CACCAACACTACACCGTCGAGGTGGGGGACACCAACTTCACCATCCACAGTCGGTACATT 60
5680R-1.IR_full 61 AATCTACGGCCCATAGGATCAGGTGCCCAAGGAATAGTATGCGCCGCTTACGATACTATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 61 AATCTACGGCCCATAGGATCAGGTGCCCAAGGAATAGTATGCGCCGCTTACGATACTATC 120
5680R-1.IR_full 121 ACCCAGCAAAATGTGGCGATTAAGAAACTATCTCGACCATTCCAAAATGTAACCCATGCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 121 ACCCAGCAAAATGTGGCGATTAAGAAACTATCTCGACCATTCCAAAATGTAACCCATGCC 180
5680R-1.IR_full 181 AAGCGAGCATATAGGGAATTCAAGCTAATGAAGCTTGTTAACCATAAAAACATAATTGGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 181 AAGCGAGCATATAGGGAATTCAAGCTAATGAAGCTTGTTAACCATAAAAACATAATTGGC 240
5680R-1.IR_full 241 TTACTTAATGCTTTTACGCCGCAAAGGAACTTGGAAGAGTTTCAGGATGTCTACCTGGTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 241 TTACTTAATGCTTTTACGCCGCAAAGGAACTTGGAAGAGTTTCAGGATGTCTACCTGGTC 300
5680R-1.IR_full 301 ATGGAGCTGATGGACGCTAATCTCTGCCAGGTCATCCAAATGGACTTGGATCACGACAGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 301 ATGGAGCTGATGGACGCTAATCTCTGCCAGGTCATCCAAATGGACTTGGATCACGACAGA 360
5680R-1.IR_full 361 ATGTCCTATTTGCTTTATCAAATGTTGTGCGGAATAAAACATTTACACTCAGCAGGAATT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 361 ATGTCCTATTTGCTTTATCAAATGTTGTGCGGAATAAAACATTTACACTCAGCAGGAATT 420
5680R-1.IR_full 421 ATTCACAGAGACTTAAAGCCATCCAATATAGTTGTAAAGGCCGACTGCACTCTAAAAATT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5680R-1.in silico 421 ATTCACAGAGACTTAAAGCCATCCAATATAGTTGTAAAGGCCGACTGCACTCTAAAAATT 480
5680R-1.IR_full 481 TTGGATTTCGGTCTGGCACG 500
||||||||||||||||||||
5680R-1.in silico 481 TTGGATTTCGGTCTGGCACG 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164900.1 |
CG5680-RB (bsk), mRNA |
| 0.2 |
1 |
3 |
3 |
2 |
NM_141972.1 |
CG5999-RA (CG5999), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057981.3 |
CG4063-RA (ebi), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_170424.1 |
CG7609-RA, transcript variant A (CG7609), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_143463.3 |
CG7609-RB, transcript variant B (CG7609), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141774.2 |
CG4596-RA (CG4596), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_206554.1 |
CG33338-RA (p38c), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_168126.1 |
CG5406-RB, transcript variant B (sif), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_079572.2 |
CG9484-RA (hyd), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_132805.1 |
CG6324-RA (CG6324), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014541.1 |
CG8201-RM, transcript variant M (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_143038.1 |
CG13636-RA, transcript variant A (CG13636), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206178.1 |
CG8201-RA, transcript variant A (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206173.2 |
CG8201-RC, transcript variant C (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206177.1 |
CG8201-RB, transcript variant B (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014542.1 |
CG8201-RL, transcript variant L (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014540.1 |
CG8201-RN, transcript variant N (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206171.2 |
CG8201-RH, transcript variant H (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206170.2 |
CG8201-RI, transcript variant I (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206169.2 |
CG8201-RJ, transcript variant J (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206168.2 |
CG8201-RO, transcript variant O (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206175.2 |
CG8201-RF, transcript variant F (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206176.2 |
CG8201-RD, transcript variant D (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206174.2 |
CG8201-RE, transcript variant E (par-1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206172.1 |
CG8201-RG, transcript variant G (par-1), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141199.2 |
CG1103-RA, transcript variant A (CG1103), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001043195.1 |
CG1103-RB, transcript variant B (CG1103), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_206278.1 |
CG5406-RC, transcript variant C (sif), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079908.2 |
CG5406-RA, transcript variant A (sif), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_138258.2 |
CG2211-RA (CG2211), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04013
:
MAPK signaling pathway - fly
dme04068
:
FoxO signaling pathway
dme04137
:
Mitophagy - animal
dme04140
:
Autophagy - animal
dme04141
:
Protein processing in endoplasmic reticulum
dme04214
:
Apoptosis - fly
dme04215
:
Apoptosis - multiple species
dme04310
:
Wnt signaling pathway
dme04391
:
Hippo signaling pathway - fly
dme04624
:
Toll and Imd signaling pathway
|
|
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