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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5576R-1 |
| Symbol |
imd |
Full Name |
immune deficiency |
| CG No |
CG5576 |
Old CG No |
CG5576 |
| Synonyms |
CG5576, IMD, Imd, BG5, shadok, unnamed, anon-WO0172774.166, imd |
| Accession No (Link to NCBI) |
NM_133166.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
male puapl lethal, female pupal semi-lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Wu C, Chen C, Dai J, Zhang F, Chen Y, Li W, Pastor-Pareja JC, Xue L.
Toll pathway modulates TNF-induced JNK-dependent cell death in Drosophila.
Open Biol (2015) 5(7) 140171
[
PubMed ID = 26202785
]
[
RRC reference
]
Bangi E, Pitsouli C, Rahme LG, Cagan R, Apidianakis Y.
Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells.
EMBO Rep (2012) 13(6) 569-76
[
PubMed ID = 22498775
]
[
RRC reference
]
Nelson B, Freisinger T, Ishii K, Okado K, Shinzawa N, Fukumoto S, Kanuka H.
Activation of Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila.
Biochem Biophys Res Commun (2013) 430(3) 1120-5
[
PubMed ID = 23261474
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCTTTGGCGGGAAGGAGGCA |
| Primer Seq. 3' |
aatctagaggtaccACATCCGGTTCCTCTTGTGAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATCTTTGGCG GGAAGGAGGC ACAGAATCCG ACACCCGTCG AGGGACGCCT GGAAAAGGAC
0061 GCAGCTCCCG TGGACGACAA CGAACCAGAT AACAACAACA GCGGAGCCAT GGCGCTGCCC
0121 TCCACCGCTG GCACGCCCAC AGCCTCCTCG GATCTGACCG AATCCGTGCT GCGCGAGCTC
0181 AGCGACCCAA ACTACAATTC AATGGCATGT GGTGCATTCC GCCAATATTC CGGGCACTCT
0241 CAGTAACGTC CAGACAAACA ACACCATGAA CGTACACAGC GCCCAGCAAC AGGTGGTCAT
0301 GAACTTCTCG AATGCCAATA ATCTGCACTT CGGCTCCGTC TACAACTTCA ACCAAAACTT
0361 GAGCGCCTGC AGCTCGCGAA AGGGTAGCAC CAGTACCGCA GAGGAGTCGG TCGCCTCTCC
0421 AGACGGTAAG CCGCGGGCAA GTgCGACGCG CAAAACGGTC AGCATTGTGG CCATGATGCA
0481 GTCACAAGAG GAACCGGATG T |
| in silico PCR Fragment |
0001 ATCTTTGGCG GGAAGGAGGC ACAGAATCCG ACACCCGTCG AGGGACGCCT GGAAAAGGAC
0061 GCAGCTCCCG TGGACGACAA CGAACCAGAT AACAACAACA GCGGAGCCCT GGCGCTGCCC
0121 TCCACCGCTG GCACGCCCAC AGCCTCCTCG GATCTGACCG AATCCGTGCT GCGCGAGCTC
0181 AGCGACCCAA ACTACAATTC AATGG-ATGT GGTGCATTCC GCCAATATTC CGGGCACTCT
0241 CAGTAACGTC CAGACAAACA ACACCATGAA CGTACACAGC GCCCAGCAAC AGGTGGTCAT
0301 GAACTTCTCG AATGCCAATA ATCTGCACTT CGGCTCCGTC TACAACTTCA ACCAAAACTT
0361 GAGCGCCTGC AGCTCGCGAA AGGGTAGCAC CAGTACCGCA GAGGAGTCGG TCGCCTCTCC
0421 AGACGGTAAG CCGCGGGCAA GTGCGACGCG CAAAACGGTC AGCATTGTGG CCATGATGCA
0481 GTCACAAGAG GAACCGGATG T |
| Assemble Data |
5576R-1.IR_full 1 ATCTTTGGCGGGAAGGAGGCACAGAATCCGACACCCGTCGAGGGACGCCTGGAAAAGGAC 60
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5576R-1.in silico 1 ATCTTTGGCGGGAAGGAGGCACAGAATCCGACACCCGTCGAGGGACGCCTGGAAAAGGAC 60
5576R-1.IR_full 61 GCAGCTCCCGTGGACGACAACGAACCAGATAACAACAACAGCGGAGCCATGGCGCTGCCC 120
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5576R-1.in silico 61 GCAGCTCCCGTGGACGACAACGAACCAGATAACAACAACAGCGGAGCCCTGGCGCTGCCC 120
5576R-1.IR_full 121 TCCACCGCTGGCACGCCCACAGCCTCCTCGGATCTGACCGAATCCGTGCTGCGCGAGCTC 180
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5576R-1.in silico 121 TCCACCGCTGGCACGCCCACAGCCTCCTCGGATCTGACCGAATCCGTGCTGCGCGAGCTC 180
5576R-1.IR_full 181 AGCGACCCAAACTACAATTCAATGGCATGTGGTGCATTCCGCCAATATTCCGGGCACTCT 240
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5576R-1.in silico 181 AGCGACCCAAACTACAATTCAATGG-ATGTGGTGCATTCCGCCAATATTCCGGGCACTCT 240
5576R-1.IR_full 241 CAGTAACGTCCAGACAAACAACACCATGAACGTACACAGCGCCCAGCAACAGGTGGTCAT 300
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5576R-1.in silico 241 CAGTAACGTCCAGACAAACAACACCATGAACGTACACAGCGCCCAGCAACAGGTGGTCAT 300
5576R-1.IR_full 301 GAACTTCTCGAATGCCAATAATCTGCACTTCGGCTCCGTCTACAACTTCAACCAAAACTT 360
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5576R-1.in silico 301 GAACTTCTCGAATGCCAATAATCTGCACTTCGGCTCCGTCTACAACTTCAACCAAAACTT 360
5576R-1.IR_full 361 GAGCGCCTGCAGCTCGCGAAAGGGTAGCACCAGTACCGCAGAGGAGTCGGTCGCCTCTCC 420
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5576R-1.in silico 361 GAGCGCCTGCAGCTCGCGAAAGGGTAGCACCAGTACCGCAGAGGAGTCGGTCGCCTCTCC 420
5576R-1.IR_full 421 AGACGGTAAGCCGCGGGCAAGTGCGACGCGCAAAACGGTCAGCATTGTGGCCATGATGCA 480
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5576R-1.in silico 421 AGACGGTAAGCCGCGGGCAAGTGCGACGCGCAAAACGGTCAGCATTGTGGCCATGATGCA 480
5576R-1.IR_full 481 GTCACAAGAGGAACCGGATGT 501
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5576R-1.in silico 481 GTCACAAGAGGAACCGGATGT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
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| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_133166.2 |
CG5576-RA (imd), mRNA |
| 0.41 |
2 |
2 |
2 |
2 |
NM_079387.5 |
CG4118-RA, transcript variant A (nxf2), mRNA |
| 0.41 |
2 |
2 |
2 |
2 |
NM_168674.2 |
CG4118-RB, transcript variant B (nxf2), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_133124.1 |
CG7502-RA (CG7502), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_137977.2 |
CG11299-RA (CG11299), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079183.2 |
CG1072-RA, transcript variant A (Awh), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_168042.1 |
CG1072-RB, transcript variant B (Awh), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079449.2 |
CG7978-RA (Ac76E), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_142656.2 |
CG17838-RB, transcript variant B (CG17838), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_169928.1 |
CG17838-RC, transcript variant C (CG17838), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_135633.2 |
CG4636-RA (SCAR), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143035.3 |
CG6995-RA, transcript variant A (CG6995), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_170169.2 |
CG6995-RB, transcript variant B (CG6995), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001038977.1 |
CG6995-RC, transcript variant C (CG6995), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139554.3 |
CG12076-RA, transcript variant A (YT521-B), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168029.1 |
CG12076-RB, transcript variant B (YT521-B), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143668.2 |
CG1981-RA (Thd1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166269.1 |
CG5119-RG, transcript variant G (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166266.1 |
CG5119-RD, transcript variant D (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166264.1 |
CG5119-RB, transcript variant B (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166268.2 |
CG5119-RF, transcript variant F (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057319.3 |
CG5119-RA, transcript variant A (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206160.1 |
CG5119-RH, transcript variant H (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166267.1 |
CG5119-RE, transcript variant E (pAbp), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166265.1 |
CG5119-RC, transcript variant C (pAbp), mRNA |
| 0 |
0 |
0 |
5 |
15 |
NM_142162.2 |
CG6912-RA (CG6912), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_057267.3 |
CG9579-RA (AnnX), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_134871.1 |
CG3131-RA (Duox), mRNA |
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