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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5485R-3 |
| Symbol |
Prestin |
Full Name |
Prestin |
| CG No |
CG5485 |
Old CG No |
CG5485 |
| Synonyms |
dpres, CG5485, Prestin |
| Accession No (Link to NCBI) |
NM_140767.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Hirata T, Cabrero P, Berkholz DS, Bondeson DP, Ritman EL, Thompson JR, Dow JA, Romero MF.
In vivo Drosophilia genetic model for calcium oxalate nephrolithiasis.
Am J Physiol Renal Physiol (2012) 303(11) F1555-62
[
PubMed ID = 22993075
]
[
RRC reference
]
Xu C, Franklin B, Tang HW, Regimbald-Dumas Y, Hu Y, Ramos J, Bosch JA, Villalta C, He X, Perrimon N.
An in vivo RNAi screen uncovers the role of AdoR signaling and adenosine deaminase in controlling intestinal stem cell activity.
Proc Natl Acad Sci U S A (2020) 117(1) 464-471
[
PubMed ID = 31852821
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTACAAATGGAAACGGTGGCAG |
| Primer Seq. 3' |
aatctagaggtaccAACCGAAGGCGTTAATAGGCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TACAAATGGA AACGGTGGCA GTGTACCAAG CAAGCCAAAG ATCCAGCCAA AATATAGCAT
0061 TCACCGAGAT GTGCTGACCC ATGAGGTGGT GATAAAGCAA ACAGGATATG CGGCCAGGGA
0121 CAAGAGCATA CCCTCCTCGC TGCGAAACTG CTGGCGGAGC TGGNAACTTC TTTGCACTCT
0181 TCACCGGCGT GATTCCCATT CTGCAATGGC TACCCCAGTA TTCACCCAGG AGAGATCTTC
0241 CTGGCGATAT ANATCGCCGG CTTCACCGTC GCCATCATGA ACATACCTCA TGGCATGGCN
0301 TTATGGCATT CTAGCAGGAG TTTCGGCTGG AAATGGTCTC TATATGGCCG TGTTTCCGGT
0361 GCTGGCCTAC ATGTTCCTGG GGACCTCCAA GCACATCTCC ATCGGCACCT TTGCGGTGGC
0421 CAGCATGATG ACGGCAAAGG TGGTGGATAC GTATGCCAAC GTAGATGACC ACCATCAGAT
0481 ATTGCCTATT AACGCCTTCG GTT |
| in silico PCR Fragment |
0001 TACAAATGGA AACGGTGGCA GTGTACCAAG CAAGCCAAAG ATCCAGCCAA AATATAGCAT
0061 TCACCGAGAT GTGCTGACCC ATGAGGTGGT GATAAAGCAA ACAGGATATG CGGCCAGGGA
0121 CAAGAGCATA CCCTCCTCGC TGCGAAACTG CTGGCGGAGC TGG-AACTTC TTTGCACTCT
0181 TCACCGGCGT GATTCCCATT CTGCAATGGC TACCCCAGTA TTCACCCAGG AGAGATCTTC
0241 CTGGCGATAT A-ATCGCCGG CTTCACCGTC GCCATCATGA ACATACCTCA TGGCATGGC-
0301 TTATGGCATT CTAGCAGGAG TTTCGGCTGG AAATGGTCTC TATATGGCCG TGTTTCCGGT
0361 GCTGGCCTAC ATGTTCCTGG GGACCTCCAA GCACATCTCC ATCGGCACTT TTGCGGTGGC
0421 CAGCATGATG ACGGCAAAGG TGGTGGATAC GTATGCCAAC GTAGATGACC ACCATCAGAT
0481 ATTGCCTATT AACGCCTTCG GTT |
| Assemble Data |
5485R-3.IR_full 1 TACAAATGGAAACGGTGGCAGTGTACCAAGCAAGCCAAAGATCCAGCCAAAATATAGCAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 1 TACAAATGGAAACGGTGGCAGTGTACCAAGCAAGCCAAAGATCCAGCCAAAATATAGCAT 60
5485R-3.IR_full 61 TCACCGAGATGTGCTGACCCATGAGGTGGTGATAAAGCAAACAGGATATGCGGCCAGGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 61 TCACCGAGATGTGCTGACCCATGAGGTGGTGATAAAGCAAACAGGATATGCGGCCAGGGA 120
5485R-3.IR_full 121 CAAGAGCATACCCTCCTCGCTGCGAAACTGCTGGCGGAGCTGGNAACTTCTTTGCACTCT 180
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
5485R-3.in silico 121 CAAGAGCATACCCTCCTCGCTGCGAAACTGCTGGCGGAGCTGG-AACTTCTTTGCACTCT 180
5485R-3.IR_full 181 TCACCGGCGTGATTCCCATTCTGCAATGGCTACCCCAGTATTCACCCAGGAGAGATCTTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 181 TCACCGGCGTGATTCCCATTCTGCAATGGCTACCCCAGTATTCACCCAGGAGAGATCTTC 240
5485R-3.IR_full 241 CTGGCGATATANATCGCCGGCTTCACCGTCGCCATCATGAACATACCTCATGGCATGGCN 300
||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 241 CTGGCGATATA-ATCGCCGGCTTCACCGTCGCCATCATGAACATACCTCATGGCATGGC- 300
5485R-3.IR_full 301 TTATGGCATTCTAGCAGGAGTTTCGGCTGGAAATGGTCTCTATATGGCCGTGTTTCCGGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 301 TTATGGCATTCTAGCAGGAGTTTCGGCTGGAAATGGTCTCTATATGGCCGTGTTTCCGGT 360
5485R-3.IR_full 361 GCTGGCCTACATGTTCCTGGGGACCTCCAAGCACATCTCCATCGGCACCTTTGCGGTGGC 420
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
5485R-3.in silico 361 GCTGGCCTACATGTTCCTGGGGACCTCCAAGCACATCTCCATCGGCACTTTTGCGGTGGC 420
5485R-3.IR_full 421 CAGCATGATGACGGCAAAGGTGGTGGATACGTATGCCAACGTAGATGACCACCATCAGAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5485R-3.in silico 421 CAGCATGATGACGGCAAAGGTGGTGGATACGTATGCCAACGTAGATGACCACCATCAGAT 480
5485R-3.IR_full 481 ATTGCCTATTAACGCCTTCGGTT 503
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5485R-3.in silico 481 ATTGCCTATTAACGCCTTCGGTT 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140767.1 |
CG5485-RA (Prestin), mRNA |
| 0 |
0 |
3 |
2 |
16 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
3 |
2 |
11 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_134789.1 |
CG15356-RA (CG15356), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_166402.1 |
CG30147-RB, transcript variant B (Hil), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137649.2 |
CG30147-RA, transcript variant A (Hil), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_137226.2 |
CG8314-RA (CG8314), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080152.2 |
CG5092-RA (Tor), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137809.1 |
CG3292-RA (CG3292), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130657.2 |
CG13762-RA (CG13762), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_137251.2 |
CG8443-RA (CG8443), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166742.1 |
CG31998-RA (CG31998), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136448.2 |
CG2144-RA (CG2144), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164987.1 |
CG14945-RB, transcript variant B (CG14945), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135697.1 |
CG14945-RA, transcript variant A (CG14945), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_176479.1 |
CG4381-RA (GstD3), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_166941.1 |
CG32796-RA, transcript variant A (CG32796), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_166940.1 |
CG32796-RB, transcript variant B (CG32796), mRNA |
| 0 |
0 |
0 |
6 |
14 |
NM_165881.1 |
CG8487-RA, transcript variant A (garz), mRNA |
| 0 |
0 |
0 |
6 |
14 |
NM_136917.2 |
CG8487-RB, transcript variant B (garz), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_167535.1 |
CG9676-RA (CG9676), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_175955.1 |
CG33122-RA (cutlet), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_138084.2 |
CG13585-RA, transcript variant A (CG13585), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_166676.1 |
CG13585-RB, transcript variant B (CG13585), mRNA |
| 0 |
0 |
0 |
3 |
18 |
NM_141738.3 |
CG6303-RA (Bruce), mRNA |
| 0 |
0 |
0 |
3 |
15 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
0 |
3 |
12 |
NM_137979.2 |
CG13560-RA (CG13560), mRNA |
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